PMID- 33390420
OWN - NLM
STAT- MEDLINE
DCOM- 20211117
LR  - 20211117
IS  - 1348-4540 (Electronic)
IS  - 0918-8959 (Linking)
VI  - 68
IP  - 4
DP  - 2021 Apr 28
TI  - Comparative proteomic analysis to identify the novel target gene of angiotensin 
      II in adrenocortical H295R cells.
PG  - 441-450
LID - 10.1507/endocrj.EJ20-0144 [doi]
AB  - Angiotensin II (Ang II) is a well-known peptide that maintains the balance of 
      electrolytes in the higher vertebrates. Ang II stimulation in the adrenal gland 
      induces the synthesis of mineralocorticoids, mainly aldosterone, through the 
      up-regulation of aldosterone synthase (CYP11B2) gene expression. Additionally, it 
      has been reported that Ang II activates multiple signaling pathways such as 
      mitogen-activated protein kinase (MAPK) and Ca(2+) signaling. Although Ang II has 
      various effects on the cellular signaling in the adrenal cells, its biological 
      significance, except for the aldosterone synthesis, is still unclear. In this 
      study, we attempted to search the novel target gene(s) of Ang II in the human 
      adrenal H295R cells using a proteomic approach combined with stable isotopic 
      labeling using amino acid in cell culture (SILAC). Interestingly, we found that 
      Ang II stimulation elevated the expression of phosphofructokinase type platelet 
      (PFKP) in both protein and mRNA levels. Moreover, transactivation of PFKP by Ang 
      II was dependent on extracellular-signal-regulated kinase (ERK) 1/2 activation. 
      Finally, we observed that Ang II treatment facilitated glucose uptake in the 
      H295R cells. Taken together, we here identified PFKP as a novel target gene of 
      Ang II, indicating that Ang II not only stimulates steroidogenesis but also 
      affects glucose metabolism.
FAU - Ito, Ryo
AU  - Ito R
AD  - Department of Molecular Endocrinology, Tohoku University Graduate School of 
      Medicine, Sendai 980-8575, Japan.
FAU - Shima, Hiroki
AU  - Shima H
AD  - Department of Biochemistry, Tohoku University Graduate School of Medicine, Sendai 
      980-8575, Japan.
FAU - Masuda, Koji
AU  - Masuda K
AD  - Research Center for Epigenetic Disease, Institute of Molecular and Cellular 
      Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.
FAU - Sato, Ikuko
AU  - Sato I
AD  - Department of Molecular Endocrinology, Tohoku University Graduate School of 
      Medicine, Sendai 980-8575, Japan.
FAU - Shimada, Hiroki
AU  - Shimada H
AD  - Department of Molecular Endocrinology, Tohoku University Graduate School of 
      Medicine, Sendai 980-8575, Japan.
FAU - Yokoyama, Atsushi
AU  - Yokoyama A
AD  - Department of Molecular Endocrinology, Tohoku University Graduate School of 
      Medicine, Sendai 980-8575, Japan.
FAU - Shirahige, Katsuhiko
AU  - Shirahige K
AD  - Research Center for Epigenetic Disease, Institute of Molecular and Cellular 
      Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.
FAU - Igarashi, Kazuhiko
AU  - Igarashi K
AD  - Department of Biochemistry, Tohoku University Graduate School of Medicine, Sendai 
      980-8575, Japan.
FAU - Sugawara, Akira
AU  - Sugawara A
AD  - Department of Molecular Endocrinology, Tohoku University Graduate School of 
      Medicine, Sendai 980-8575, Japan.
LA  - eng
PT  - Journal Article
DEP - 20201226
PL  - Japan
TA  - Endocr J
JT  - Endocrine journal
JID - 9313485
RN  - 11128-99-7 (Angiotensin II)
RN  - EC 1.14.15.4 (Cytochrome P-450 CYP11B2)
SB  - IM
MH  - Adrenal Cortex/*drug effects/metabolism
MH  - Angiotensin II/pharmacology
MH  - Cell Line
MH  - Cytochrome P-450 CYP11B2/*genetics/metabolism
MH  - Gene Expression/*drug effects
MH  - Humans
MH  - Proteomics
MH  - Signal Transduction/drug effects
MH  - Up-Regulation/drug effects
OTO - NOTNLM
OT  - Adrenocortical cell
OT  - Angiotensin II
OT  - Phosphofructokinase type platelet
OT  - Proteome analysis
EDAT- 2021/01/05 06:00
MHDA- 2021/11/18 06:00
CRDT- 2021/01/04 05:24
PHST- 2021/01/05 06:00 [pubmed]
PHST- 2021/11/18 06:00 [medline]
PHST- 2021/01/04 05:24 [entrez]
AID - 10.1507/endocrj.EJ20-0144 [doi]
PST - ppublish
SO  - Endocr J. 2021 Apr 28;68(4):441-450. doi: 10.1507/endocrj.EJ20-0144. Epub 2020 
      Dec 26.