PMID- 33391243
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210106
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 11
DP  - 2020
TI  - Signal Recognition Particle RNA Contributes to Oxidative Stress Response in 
      Deinococcus radiodurans by Modulating Catalase Localization.
PG  - 613571
LID - 10.3389/fmicb.2020.613571 [doi]
LID - 613571
AB  - The proper functioning of many proteins requires their transport to the correct 
      cellular compartment or their secretion. Signal recognition particle (SRP) is a 
      major protein transport pathway responsible for the co-translational movement of 
      integral membrane proteins as well as periplasmic proteins. Deinococcus 
      radiodurans is a ubiquitous bacterium that expresses a complex phenotype of 
      extreme oxidative stress resistance, which depends on proteins involved in DNA 
      repair, metabolism, gene regulation, and antioxidant defense. These proteins are 
      located extracellularly or subcellularly, but the molecular mechanism of protein 
      localization in D. radiodurans to manage oxidative stress response remains 
      unexplored. In this study, we characterized the SRP complex in D. radiodurans R1 
      and showed that the knockdown (KD) of the SRP RNA (Qpr6) reduced bacterial 
      survival under hydrogen peroxide and growth under chronic ionizing radiation. 
      Through LC-mass spectrometry (MS/MS) analysis, we detected 162 proteins in the 
      periplasm of wild-type D. radiodurans, of which the transport of 65 of these 
      proteins to the periplasm was significantly reduced in the Qpr6 KD strain. 
      Through Western blotting, we further demonstrated the localization of the 
      catalases in D. radiodurans, DR_1998 (KatE1) and DR_A0259 (KatE2), in both the 
      cytoplasm and periplasm, respectively, and showed that the accumulation of KatE1 
      and KatE2 in the periplasm was reduced in the SRP-defective strains. 
      Collectively, this study establishes the importance of the SRP pathway in the 
      survival and the transport of antioxidant proteins in D. radiodurans under 
      oxidative stress.
CI  - Copyright (c) 2020 Han, Fang, Jiang, Gaidamakova, Tkavc, Daly and Contreras.
FAU - Han, Runhua
AU  - Han R
AD  - McKetta Department of Chemical Engineering, The University of Texas at Austin, 
      Austin, TX, United States.
FAU - Fang, Jaden
AU  - Fang J
AD  - McKetta Department of Chemical Engineering, The University of Texas at Austin, 
      Austin, TX, United States.
FAU - Jiang, Jessie
AU  - Jiang J
AD  - McKetta Department of Chemical Engineering, The University of Texas at Austin, 
      Austin, TX, United States.
FAU - Gaidamakova, Elena K
AU  - Gaidamakova EK
AD  - Uniformed Services University of the Health Sciences, Department of Pathology, 
      Bethesda, MD, United States.
AD  - The Henry M. Jackson Foundation for the Advancement of Military Medicine, 
      Bethesda, MD, United States.
FAU - Tkavc, Rok
AU  - Tkavc R
AD  - Uniformed Services University of the Health Sciences, Department of Pathology, 
      Bethesda, MD, United States.
AD  - The Henry M. Jackson Foundation for the Advancement of Military Medicine, 
      Bethesda, MD, United States.
AD  - Uniformed Services University of the Health Sciences, Department of Microbiology 
      and Immunology, Bethesda, MD, United States.
FAU - Daly, Michael J
AU  - Daly MJ
AD  - Uniformed Services University of the Health Sciences, Department of Pathology, 
      Bethesda, MD, United States.
FAU - Contreras, Lydia M
AU  - Contreras LM
AD  - McKetta Department of Chemical Engineering, The University of Texas at Austin, 
      Austin, TX, United States.
AD  - Institute for Cellular & Molecular Biology, The University of Texas at Austin, 
      Austin, TX, United States.
LA  - eng
GR  - R21 ES025923/ES/NIEHS NIH HHS/United States
PT  - Journal Article
DEP - 20201218
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC7775534
OTO - NOTNLM
OT  - Deinococcus radiodurans
OT  - catalase
OT  - oxidative stress
OT  - periplasm
OT  - protein transport
OT  - signal recognition particle
OT  - small non-coding RNA
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/01/05 06:00
MHDA- 2021/01/05 06:01
PMCR- 2020/12/18
CRDT- 2021/01/04 05:27
PHST- 2020/10/02 00:00 [received]
PHST- 2020/11/27 00:00 [accepted]
PHST- 2021/01/04 05:27 [entrez]
PHST- 2021/01/05 06:00 [pubmed]
PHST- 2021/01/05 06:01 [medline]
PHST- 2020/12/18 00:00 [pmc-release]
AID - 10.3389/fmicb.2020.613571 [doi]
PST - epublish
SO  - Front Microbiol. 2020 Dec 18;11:613571. doi: 10.3389/fmicb.2020.613571. 
      eCollection 2020.