PMID- 33391885
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220419
IS  - 2167-8359 (Print)
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 8
DP  - 2020
TI  - Sevoflurane preconditioning attenuates hypoxia/reoxygenation injury of H9c2 
      cardiomyocytes by activation of the HIF-1/PDK-1 pathway.
PG  - e10603
LID - 10.7717/peerj.10603 [doi]
LID - e10603
AB  - BACKGROUND: Sevoflurane preconditioning (SPC) can provide myocardial protective 
      effects similar to ischemic preconditioning (IPC). However, the underlying 
      molecular mechanism of SPC remains unclear. Studies confirm that 
      hypoxia-inducible factor-1 (HIF-1) can transform cells from aerobic oxidation to 
      anaerobic glycolysis by activating the switch protein pyruvate dehydrogenase 
      kinase-1 (PDK-1), thus providing energy for the normal life activities of cells 
      under hypoxic conditions. The purpose of this study was to investigate whether 
      the cardioprotective effects of SPC are associated with activation of the 
      HIF-1a/PDK-1 signal pathway. METHODS: The H9c2 cardiomyocytes 
      hypoxia/reoxygenation model was established and treated with 2.4% sevoflurane at 
      the end of equilibration. Lactate dehydrogenase (LDH) level, cell viability, cell 
      apoptosis, mitochondrial membrane potential, key enzymes of glycolysis, ATP 
      concentration of glycolysis were assessed after the intervention. Apoptosis 
      related protein(Bcl-2, Bax), HIF-1a protein, and PDK-1 protein were assessed by 
      western blot. RESULTS: Compared with the H/R group, SPC significantly increased 
      the expression of HIF-1a, PDK-1, and Bcl-2 and reduced the protein expression of 
      Bax, which markedly decreased the apoptosis ratio and Lactate dehydrogenase (LDH) 
      level, increasing the cell viability, content of key enzymes of glycolysis, ATP 
      concentration of glycolysis and stabilizing the mitochondrial membrane potential. 
      However, the cardioprotective effects of SPC were disappeared by treatment with a 
      HIF-1a selective inhibitor. CONCLUSION: This study demonstrates that the 
      cardioprotective effects of SPC are associated with the activation of the 
      HIF-1a/PDK-1 signaling pathway. The mechanism may be related to increasing the 
      content of key enzymes and ATP of glycolysis in the early stage of hypoxia.
CI  - (c)2020 Hou et al.
FAU - Hou, Tianliang
AU  - Hou T
AD  - Department of Anesthesiology, The First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang, China.
FAU - Ma, Haiping
AU  - Ma H
AD  - Department of Anesthesiology, The First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang, China.
FAU - Wang, Haixia
AU  - Wang H
AD  - Department of Mastology, Xinjiang Maternal and Child Health Hospital, Urumqi, 
      Xinjiang, China.
FAU - Chen, Chunling
AU  - Chen C
AD  - Department of Anesthesiology, The First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang, China.
FAU - Ye, Jianrong
AU  - Ye J
AD  - Department of Anesthesiology, The First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang, China.
FAU - Ahmed, Ahmed Mohamed
AU  - Ahmed AM
AD  - Department of Intensive Care Unit (ICU), Yardimeli Hospital, Mogadishu, Somalia.
FAU - Zheng, Hong
AU  - Zheng H
AD  - Department of Anesthesiology, The First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang, China.
LA  - eng
PT  - Journal Article
DEP - 20201221
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
PMC - PMC7759118
OTO - NOTNLM
OT  - Glycolysis
OT  - Hypoxia-inducible factor-1
OT  - Hypoxia/reoxygenation injury
OT  - Myocardial protective effect
OT  - Pyruvate dehydrogenase kinase-1
OT  - Sevoflurane preconditioning
COIS- The authors declare there are no competing interests.
EDAT- 2021/01/05 06:00
MHDA- 2021/01/05 06:01
PMCR- 2020/12/21
CRDT- 2021/01/04 05:29
PHST- 2020/09/03 00:00 [received]
PHST- 2020/11/28 00:00 [accepted]
PHST- 2021/01/04 05:29 [entrez]
PHST- 2021/01/05 06:00 [pubmed]
PHST- 2021/01/05 06:01 [medline]
PHST- 2020/12/21 00:00 [pmc-release]
AID - 10603 [pii]
AID - 10.7717/peerj.10603 [doi]
PST - epublish
SO  - PeerJ. 2020 Dec 21;8:e10603. doi: 10.7717/peerj.10603. eCollection 2020.