PMID- 33392630 OWN - NLM STAT- MEDLINE DCOM- 20211124 LR - 20211124 IS - 1432-184X (Electronic) IS - 0095-3628 (Linking) VI - 81 IP - 4 DP - 2021 May TI - Biofilms of Pseudomonas and Lysinibacillus Marine Strains on High-Density Polyethylene. PG - 833-846 LID - 10.1007/s00248-020-01666-8 [doi] AB - Environmental pollution by plastic debris is estimated on a scale of 100 million metric tons, a portion of which is fragmented into micro- and nanoplastics. These fragments are often colonized by bacterial species in marine environments, possibly contributing to the biodegradation of such materials. However, further investigations are necessary to determine the impact of abiotic polymer weathering on biofilm adhesion, as well as the specific biofilm formation strategies employed by marine isolates. Here, we evaluate deep-sea sediment bacterial isolates for biofilm adhesion, extracellular matrix production, and polymer degradation ability. Our study focuses on high-density polyethylene (HDPE) fragments for their high durability and environmental persistence, subjecting fragments to abiotic weathering prior to bacterial colonization. Marine isolates identified as Pseudomonas sp. and Lysinibacillus sp. exhibited decreasing biofilm formation on weathered HDPE, especially over the first 24 h of incubation. This effect was countered by increased extracellular matrix production, likely improving cell adhesion to surfaces roughened by abiotic degradation. These adhesion strategies were contrasted with a reference Pseudomonas aeruginosa strain, which displayed high levels of biofilm formation on non-weathered HDPE and lower extracellular matrix production over the first 24 h of incubation. Furthermore, our results suggest that an increase in biofilm biomass correlated with changes to HDPE structure, indicating that these strains have a potential for biodegradation of plastic fragments. Therefore, this work provides a detailed account of biofilm formation strategies and bacteria-plastic interactions that represent crucial steps in the biodegradation of plastic fragments in marine environments. FAU - Oliveira, Maiara Monteiro AU - Oliveira MM AD - Geobiology Laboratory, Institute of Petroleum and Natural Resources, Pontifical Catholic University of Rio Grande do Sul, PUCRS, Av. Ipiranga 6681, Building 96J, Porto Alegre, RS, 90619-900, Brazil. AD - Immunology and Microbiology Laboratory, School of Health and Life Sciences, Pontifical Catholic University of Rio Grande do Sul, PUCRS, Av. Ipiranga 6681, Building 11, Porto Alegre, RS, 90619-900, Brazil. FAU - Proenca, Audrey Menegaz AU - Proenca AM AD - Geobiology Laboratory, Institute of Petroleum and Natural Resources, Pontifical Catholic University of Rio Grande do Sul, PUCRS, Av. Ipiranga 6681, Building 96J, Porto Alegre, RS, 90619-900, Brazil. AD - Immunology and Microbiology Laboratory, School of Health and Life Sciences, Pontifical Catholic University of Rio Grande do Sul, PUCRS, Av. Ipiranga 6681, Building 11, Porto Alegre, RS, 90619-900, Brazil. FAU - Moreira-Silva, Eduardo AU - Moreira-Silva E AD - Immunology and Microbiology Laboratory, School of Health and Life Sciences, Pontifical Catholic University of Rio Grande do Sul, PUCRS, Av. Ipiranga 6681, Building 11, Porto Alegre, RS, 90619-900, Brazil. FAU - de Castro, Aline Machado AU - de Castro AM AD - Biotechnology Division, Research and Development Center (CENPES), PETROBRAS, Av. Horacio Macedo 950, Rio de Janeiro, RJ, 21941-915, Brazil. FAU - Dos Santos, Francine Melise AU - Dos Santos FM AD - Geobiology Laboratory, Institute of Petroleum and Natural Resources, Pontifical Catholic University of Rio Grande do Sul, PUCRS, Av. Ipiranga 6681, Building 96J, Porto Alegre, RS, 90619-900, Brazil. FAU - Marconatto, Leticia AU - Marconatto L AD - Geobiology Laboratory, Institute of Petroleum and Natural Resources, Pontifical Catholic University of Rio Grande do Sul, PUCRS, Av. Ipiranga 6681, Building 96J, Porto Alegre, RS, 90619-900, Brazil. FAU - Medina-Silva, Renata AU - Medina-Silva R AUID- ORCID: 0000-0002-5466-4908 AD - Geobiology Laboratory, Institute of Petroleum and Natural Resources, Pontifical Catholic University of Rio Grande do Sul, PUCRS, Av. Ipiranga 6681, Building 96J, Porto Alegre, RS, 90619-900, Brazil. renata.medina@pucrs.br. AD - Immunology and Microbiology Laboratory, School of Health and Life Sciences, Pontifical Catholic University of Rio Grande do Sul, PUCRS, Av. Ipiranga 6681, Building 11, Porto Alegre, RS, 90619-900, Brazil. renata.medina@pucrs.br. LA - eng GR - 001/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/ GR - 0050.0096017.15.9/Petrobras/ PT - Journal Article DEP - 20210103 PL - United States TA - Microb Ecol JT - Microbial ecology JID - 7500663 RN - 9002-88-4 (Polyethylene) SB - IM MH - Bacteria MH - Biodegradation, Environmental MH - Biofilms MH - *Polyethylene MH - *Pseudomonas OTO - NOTNLM OT - Aquatic organisms OT - Bacteria OT - Environmental microbiology OT - Microplastics EDAT- 2021/01/05 06:00 MHDA- 2021/11/25 06:00 CRDT- 2021/01/04 05:32 PHST- 2020/07/29 00:00 [received] PHST- 2020/12/15 00:00 [accepted] PHST- 2021/01/05 06:00 [pubmed] PHST- 2021/11/25 06:00 [medline] PHST- 2021/01/04 05:32 [entrez] AID - 10.1007/s00248-020-01666-8 [pii] AID - 10.1007/s00248-020-01666-8 [doi] PST - ppublish SO - Microb Ecol. 2021 May;81(4):833-846. doi: 10.1007/s00248-020-01666-8. Epub 2021 Jan 3.