PMID- 33400271
OWN - NLM
STAT- MEDLINE
DCOM- 20210518
LR  - 20220531
IS  - 1600-0765 (Electronic)
IS  - 0022-3484 (Linking)
VI  - 56
IP  - 3
DP  - 2021 Jun
TI  - IFN-gamma or IL-4 polarization impacts the response of gingival fibroblasts to oral 
      bacteria.
PG  - 462-470
LID - 10.1111/jre.12837 [doi]
AB  - BACKGROUND AND OBJECTIVE: We previously reported that gingival fibroblasts (GFs) 
      can be polarized into functionally distinct subtypes, immune-activating but 
      tissue-destructive or tissue-reparative, in response to T helper (Th1) and Th2 
      stimuli, respectively. The purpose of this study was to evaluate the effect of 
      polarization on GFs responses to oral bacteria. METHODS: Unprimed (GF(-)) and 
      IFN-gamma (GF(IFN-gamma)) or IL-4 primed (GF(IL-4)) GFs were stimulated with live 
      Fusobacterium nucleatum or Porphyromonas gingivalis. The mRNA expression of 
      IL-1beta, IL-4, LPS-recognizing components (Toll-like receptor (TLR) 4, CD14), 
      molecules involved in antigen presentation (human leukocyte antigen (HLA)-ABC, 
      HLA-DP, CD74, CD40), chemokines (C-X-C motif chemokine (CXCL)10, CXCL11, 
      chemokine (C-C motif) ligand 20 (CCL20)), collagen type 1 alpha 1 (COL1A1), and 
      matrix metalloproteinase (MMP)-1, and the protein levels of IL-1beta, CD14, CXCL11, 
      CCL20, and COL1A1 accumulated in supernatants were analyzed using real-time PCR 
      and ELISA. RESULTS: In response to oral bacteria, the GF(IFN-gamma) significantly 
      upregulated the expression of LPS-recognizing components, molecules involved in 
      antigen presentation, CXCL10, and CXCL11, whereas the levels of IL-4 and COL1A1 
      were downregulated, compared with GF(-). The levels of IL-1beta, CCL20, and MMP-1 
      from GF(IFN-gamma) were differently regulated between both bacteria; F. nucleatum was 
      synergistically upregulated, but P. gingivalis was downregulated. The GF(IL-4) 
      stimulated with both bacteria upregulated the levels of IL-4, whereas the levels 
      of TLR4 and chemokines were downregulated, compared with GF(-). The regulation of 
      IL-1beta, CD14, CXCL11, CCL20, and COL1A1 proteins showed a similar tendency with 
      mRNA regulation. CONCLUSION: Polarization of GFs with IFN-gamma or IL-4 affected the 
      way that GFs responded to oral bacteria through up or downregulation of 
      inflammatory responses and extracellular matrix control.
CI  - (c) 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Jang, Ju Young
AU  - Jang JY
AD  - Department of Periodontology, Institute of Oral Health Science, Ajou University 
      School of Medicine, Suwon, Korea.
FAU - Choi, Geum Hee
AU  - Choi GH
AD  - Department of Periodontology, Institute of Oral Health Science, Ajou University 
      School of Medicine, Suwon, Korea.
FAU - Ji, Suk
AU  - Ji S
AUID- ORCID: 0000-0001-9720-6731
AD  - Department of Periodontology, Institute of Oral Health Science, Ajou University 
      School of Medicine, Suwon, Korea.
LA  - eng
GR  - National Research Foundation of Korea (NRF)/
GR  - NRF-2019R1F1A1058459/Ministry of Education, Science and Technology/
PT  - Journal Article
DEP - 20210105
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
RN  - 0 (IL4 protein, human)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Fibroblasts
MH  - Fusobacterium nucleatum
MH  - *Gingiva
MH  - Humans
MH  - Interferon-gamma/*analysis
MH  - Interleukin-4/*analysis
MH  - Porphyromonas gingivalis
OTO - NOTNLM
OT  - Fusobacterium nucleatum
OT  - Porphyromonas gingivalis
OT  - chemokines
OT  - fibroblast
OT  - imflammatory mediator
OT  - innate immunology
EDAT- 2021/01/06 06:00
MHDA- 2021/05/19 06:00
CRDT- 2021/01/05 12:18
PHST- 2020/12/08 00:00 [revised]
PHST- 2020/03/30 00:00 [received]
PHST- 2020/12/15 00:00 [accepted]
PHST- 2021/01/06 06:00 [pubmed]
PHST- 2021/05/19 06:00 [medline]
PHST- 2021/01/05 12:18 [entrez]
AID - 10.1111/jre.12837 [doi]
PST - ppublish
SO  - J Periodontal Res. 2021 Jun;56(3):462-470. doi: 10.1111/jre.12837. Epub 2021 Jan 
      5.