PMID- 33401166
OWN - NLM
STAT- MEDLINE
DCOM- 20210202
LR  - 20210202
IS  - 1879-2448 (Electronic)
IS  - 0043-1354 (Linking)
VI  - 191
DP  - 2021 Mar 1
TI  - Identification of microorganisms responsible for foam formation in mesophilic 
      anaerobic digesters treating surplus activated sludge.
PG  - 116779
LID - S0043-1354(20)31312-9 [pii]
LID - 10.1016/j.watres.2020.116779 [doi]
AB  - Foaming is a common operational problem in anaerobic digestion (AD) systems, 
      where hydrophobic filamentous microorganisms are usually considered to be the 
      major cause. However, little is known about the identity of foam-stabilising 
      microorganisms in AD systems, and control measures are lacking. This study 
      identified putative foam forming microorganisms in 13 full-scale mesophilic 
      digesters located at 11 wastewater treatment plants in Denmark, using 16S rRNA 
      gene amplicon sequencing with species-level resolution and fluorescence in situ 
      hybridization (FISH) for visualization. A foaming potential aeration test was 
      applied to classify the digester sludges according to their foaming propensity. A 
      high foaming potential for sludges was linked to the abundance of species from 
      the genus Candidatus Microthrix, immigrating with the feed stream (surplus 
      activated sludge), but also to several novel phylotypes potentially growing in 
      the digester. These species were classified to the genera Ca. Brevefilum (Ca. B. 
      fermentans) and Tetrasphaera (midas_s_5), the families ST-12K33 (midas_s_22), and 
      Rikenellaceae (midas_s_141), and the archaeal genus Methanospirillum 
      (midas_s_2576). Application of FISH showed that these potential foam-forming 
      organisms all had a filamentous morphology. Additionally, it was shown that 
      concentrations of ammonium and total nitrogen correlated strongly to the presence 
      of foam-formers. This study provided new insight into the identity of putative 
      foam-forming microorganisms in mesophilic AD systems, allowing for the subsequent 
      surveillance of their abundances and studies of their ecology. Such information 
      will importantly inform the development of control measures for these problematic 
      microorganisms.
CI  - Copyright (c) 2020. Published by Elsevier Ltd.
FAU - Jiang, Chenjing
AU  - Jiang C
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University, 9220 Aalborg, Denmark; Key Laboratory of Engineering Oceanography, 
      Second Institute of Oceanography, SOA, Hangzhou, 310012, China.
FAU - McIlroy, Simon Jon
AU  - McIlroy SJ
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University, 9220 Aalborg, Denmark; Centre for Microbiome Research, School of 
      Biomedical Sciences, Queensland University of Technology (QUT), Translational 
      Research Institute, Woolloongabba, Australia.
FAU - Qi, Rong
AU  - Qi R
AD  - State Key Laboratory of Environmental Aquatic Chemistry, Research Center for 
      Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 10085, China.
FAU - Petriglieri, Francesca
AU  - Petriglieri F
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University, 9220 Aalborg, Denmark.
FAU - Yashiro, Erika
AU  - Yashiro E
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University, 9220 Aalborg, Denmark.
FAU - Kondrotaite, Zivile
AU  - Kondrotaite Z
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University, 9220 Aalborg, Denmark.
FAU - Nielsen, Per Halkjaer
AU  - Nielsen PH
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University, 9220 Aalborg, Denmark. Electronic address: phn@bio.aau.dk.
LA  - eng
PT  - Journal Article
DEP - 20201223
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - Anaerobiosis
MH  - Bioreactors
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Sewage
MH  - *Water Purification
OTO - NOTNLM
OT  - Anaerobic digestion
OT  - Foam-forming microorganism(s)
OT  - Foaming
OT  - Mesophilic
OT  - Microbial community structure
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/01/06 06:00
MHDA- 2021/02/03 06:00
CRDT- 2021/01/05 20:11
PHST- 2020/08/26 00:00 [received]
PHST- 2020/12/06 00:00 [revised]
PHST- 2020/12/20 00:00 [accepted]
PHST- 2021/01/06 06:00 [pubmed]
PHST- 2021/02/03 06:00 [medline]
PHST- 2021/01/05 20:11 [entrez]
AID - S0043-1354(20)31312-9 [pii]
AID - 10.1016/j.watres.2020.116779 [doi]
PST - ppublish
SO  - Water Res. 2021 Mar 1;191:116779. doi: 10.1016/j.watres.2020.116779. Epub 2020 
      Dec 23.