PMID- 33402471
OWN - NLM
STAT- MEDLINE
DCOM- 20210618
LR  - 20210618
IS  - 1791-7549 (Electronic)
IS  - 0258-851X (Print)
IS  - 0258-851X (Linking)
VI  - 35
IP  - 1
DP  - 2021 Jan-Feb
TI  - Curcumin Activates ROS Signaling to Promote Pyroptosis in Hepatocellular 
      Carcinoma HepG2 Cells.
PG  - 249-257
LID - 10.21873/invivo.12253 [doi]
AB  - BACKGROUND/AIM: Curcumin is a polyphenol that exerts a variety of pharmacological 
      activities and plays an anti-cancer role in many cancer cells. It was recently 
      reported that gasdermin E (GSDME) is involved in the progression of pyroptosis. 
      MATERIALS AND METHODS: HepG2 cells were treated with various concentrations of 
      curcumin and cell viability was examined using MTT assay, apoptosis was analysed 
      using flow cytometry, reactive oxygen species (ROS) levels using dihydroethidium, 
      LDH release using an LDH cytotoxicity assay, and protein expression using western 
      blot. RESULTS: Curcumin increased the expression of the GSDME N-terminus and 
      proteins involved in pyrolysis, promoted HspG2 cell pyrolysis and increased 
      intracellular ROS levels. Moreover, inhibition of the production of intracellular 
      ROS with n-acetylcysteine (NAC) improved the degree of apoptosis and pyrolysis 
      induced by curcumin. CONCLUSION: Curcumin induces HspG2 cell death by increasing 
      apoptosis and pyroptosis, and ROS play a key role in this process. This study 
      improves our understanding of the potential anti-cancer properties of curcumin in 
      liver cancer.
CI  - Copyright(c) 2021, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Liang, Wan-Feng
AU  - Liang WF
AD  - Department of Veterinary Medicine, Agricultural College of Yanbian University, 
      Yanji, P.R. China.
FAU - Gong, Yi-Xi
AU  - Gong YX
AD  - Department of Veterinary Medicine, Agricultural College of Yanbian University, 
      Yanji, P.R. China.
FAU - Li, Hai-Feng
AU  - Li HF
AD  - Department of Veterinary Medicine, Agricultural College of Yanbian University, 
      Yanji, P.R. China.
FAU - Sun, Fu-Liang
AU  - Sun FL
AD  - Department of Veterinary Medicine, Agricultural College of Yanbian University, 
      Yanji, P.R. China.
FAU - Li, Wei-Long
AU  - Li WL
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Daqing, P.R. China.
FAU - Chen, Dong-Qin
AU  - Chen DQ
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Daqing, P.R. China.
FAU - Xie, Dan-Ping
AU  - Xie DP
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Daqing, P.R. China.
FAU - Ren, Chen-Xi
AU  - Ren CX
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Daqing, P.R. China.
FAU - Guo, Xiao-Yu
AU  - Guo XY
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Daqing, P.R. China.
FAU - Wang, Zi-Yi
AU  - Wang ZY
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Daqing, P.R. China.
FAU - Kwon, Taeho
AU  - Kwon T
AD  - Primate Resources Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Jeonbuk, Republic of Korea kwon@kribb.re.kr 
      sunhunan76@163.com.
FAU - Sun, Hu-Nan
AU  - Sun HN
AD  - College of Life Science and Technology, Heilongjiang Bayi Agricultural 
      University, Daqing, P.R. China; kwon@kribb.re.kr sunhunan76@163.com.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - In Vivo
JT  - In vivo (Athens, Greece)
JID - 8806809
RN  - 0 (Reactive Oxygen Species)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Apoptosis
MH  - *Carcinoma, Hepatocellular/drug therapy/genetics
MH  - *Curcumin/pharmacology
MH  - Hep G2 Cells
MH  - Humans
MH  - *Liver Neoplasms/drug therapy/genetics
MH  - Pyroptosis
MH  - Reactive Oxygen Species
PMC - PMC7880758
OTO - NOTNLM
OT  - Curcumin
OT  - GSDME
OT  - ROS
OT  - liver cancer
OT  - proptosis
COIS- The Authors declare that there are no conflicts of interest regarding this study.
EDAT- 2021/01/07 06:00
MHDA- 2021/06/22 06:00
PMCR- 2021/01/03
CRDT- 2021/01/06 05:39
PHST- 2020/10/19 00:00 [received]
PHST- 2020/11/02 00:00 [revised]
PHST- 2020/11/03 00:00 [accepted]
PHST- 2021/01/06 05:39 [entrez]
PHST- 2021/01/07 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2021/01/03 00:00 [pmc-release]
AID - 35/1/249 [pii]
AID - 10.21873/invivo.12253 [doi]
PST - ppublish
SO  - In Vivo. 2021 Jan-Feb;35(1):249-257. doi: 10.21873/invivo.12253.