PMID- 33404558
OWN - NLM
STAT- MEDLINE
DCOM- 20210208
LR  - 20210208
IS  - 1463-9084 (Electronic)
IS  - 1463-9076 (Linking)
VI  - 23
IP  - 2
DP  - 2021 Jan 21
TI  - The importance of the bacterial cell wall in uranium(VI) biosorption.
PG  - 1566-1576
LID - 10.1039/d0cp04067c [doi]
AB  - The bacterial cell envelope, in particular the cell wall, is considered the main 
      controlling factor in the biosorption of aqueous uranium(vi) by microorganisms. 
      However, the specific roles of the cell wall, associated biomolecules, and other 
      components of the cell envelope are not well defined. Here we report findings on 
      the biosorption of uranium by isolated cell envelope components and associated 
      biomolecules, with P. putida 33015 and B. subtilis 168 investigated as 
      representative strains for the differences in Gram-negative and Gram-positive 
      cell envelope architecture, respectively. The cell wall and cell surface membrane 
      were isolated from intact cells and characterised by X-ray Photoelectron 
      Spectroscopy (XPS) and Attenuated Total Reflectance-Fourier Transform Infrared 
      (ATR-FT-IR) spectroscopy; revealing variations in the abundance of functional 
      moieties and biomolecules associated with components of the cell envelope. 
      Uranium biosorption was investigated as a function of cell envelope component and 
      pH, comparing with intact cells. The isolated cell wall from both strains 
      exhibited the greatest uranium biosorption capacity. Deprotonation of favourable 
      functional groups on the biomass as the pH increased from 3 to 5.5 increased 
      their uranium biosorption capacity by approximately 3 fold. The results from 
      ATR-FT-IR indicated that uranium(vi) biosorption was mediated by phosphate and 
      carboxyl groups associated with proteins and phosphorylated biopolymers of the 
      cell envelope. This includes outer membrane phospholipids and LPS of 
      Gram-negative bacteria and teichoic acids, surface proteins and peptidoglycan 
      from Gram-positive bacteria. As a result, the biosorption process of uranium(vi) 
      to microorganisms is controlled by surface interactions, resulting in higher 
      accumulation of uranium in the cell envelope. This demonstrates the importance of 
      bacterial cell wall as the key mediator of uranium biosorption with 
      microorganisms.
FAU - Hufton, Joseph
AU  - Hufton J
AUID- ORCID: 0000-0002-6267-3003
AD  - Department of Geography, The University of Sheffield, Sheffield, S10 2TN, UK. 
      j.m.hufton@sheffield.ac.uk.
FAU - Harding, John
AU  - Harding J
AUID- ORCID: 0000-0001-8429-3151
AD  - Department of Materials Science and Engineering, Sir Robert Hadfield Building, 
      University of Sheffield, Mappin Street, Sheffield, S1 3JD, UK.
FAU - Smith, Thomas
AU  - Smith T
AUID- ORCID: 0000-0002-4246-5020
AD  - Biomolecular Sciences Centre, Sheffield Hallam University, City Campus, Sheffield 
      S1 1WB, UK.
FAU - Romero-Gonzalez, Maria E
AU  - Romero-Gonzalez ME
AUID- ORCID: 0000-0001-5808-5383
AD  - Department of Geography, The University of Sheffield, Sheffield, S10 2TN, UK. 
      j.m.hufton@sheffield.ac.uk and School of Engineering and Materials Science 
      (SEMS), Queen Mary University of London, Mile End Road, London E1 4NS, UK. 
      m.romero-gonzalez@qmul.ac.uk.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Phys Chem Chem Phys
JT  - Physical chemistry chemical physics : PCCP
JID - 100888160
RN  - 4OC371KSTK (Uranium)
SB  - IM
MH  - Bacillus subtilis/metabolism
MH  - Cell Membrane/metabolism
MH  - Cell Wall/*metabolism
MH  - Photoelectron Spectroscopy
MH  - Pseudomonas putida/metabolism
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Uranium/*metabolism
EDAT- 2021/01/07 06:00
MHDA- 2021/02/09 06:00
CRDT- 2021/01/06 12:17
PHST- 2021/01/07 06:00 [pubmed]
PHST- 2021/02/09 06:00 [medline]
PHST- 2021/01/06 12:17 [entrez]
AID - 10.1039/d0cp04067c [doi]
PST - ppublish
SO  - Phys Chem Chem Phys. 2021 Jan 21;23(2):1566-1576. doi: 10.1039/d0cp04067c.