PMID- 33405534
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210114
IS  - 2373-9878 (Electronic)
IS  - 2373-9878 (Linking)
VI  - 5
IP  - 11
DP  - 2019 Nov 11
TI  - Sandwich-type Surface Stress Biosensor Based on Self-Assembled Gold Nanoparticles 
      in PDMS Film for BSA Detection.
PG  - 6274-6280
LID - 10.1021/acsbiomaterials.9b01073 [doi]
AB  - Poly(dimethylsiloxane) (PDMS) films composed of gold nanomaterials have drawn 
      increasing interest for their application in biosensors. However, gold and PDMS 
      exhibit poor compatibility, limiting their sensitivity in biosensing. Herein, we 
      reported a surface stress biosensor based on a gold nanoparticle-PDMS (AuNP-PDMS) 
      composite film to detect bovine serum albumin (BSA). The AuNP-PDMS film was 
      fabricated by in situ reduction of PDMS and further reduction of glucose. The 
      compatibility between gold and PDMS was improved via a two-step reduction of 
      AuNPs. The surface stress biosensor can specifically detect BSA molecules within 
      the 0-50 mug/mL concentration range using the direct assay and the sandwich assay. 
      The sandwich assay amplified the surface stress and reduced the limit of 
      detection to 0.035 mug/mL, which is lower than that achieved by the direct assay. 
      The sandwich-type biosensor also exhibits stability, repeatability, and 
      specificity. This study is expected to drive the development of new methods for 
      biomolecule detection.
FAU - Zhao, Dong
AU  - Zhao D
FAU - Zhang, Qiang
AU  - Zhang Q
FAU - Zhang, Yixia
AU  - Zhang Y
FAU - Liu, Yan
AU  - Liu Y
FAU - Pei, Zhen
AU  - Pei Z
FAU - Yuan, Zhongyun
AU  - Yuan Z
FAU - Sang, Shengbo
AU  - Sang S
AUID- ORCID: 0000-0003-3011-7632
LA  - eng
PT  - Journal Article
DEP - 20191025
PL  - United States
TA  - ACS Biomater Sci Eng
JT  - ACS biomaterials science & engineering
JID - 101654670
SB  - IM
OTO - NOTNLM
OT  - BSA detection
OT  - sandwich assay
OT  - self-assembly AuNPs
OT  - surface stress biosensor
EDAT- 2019/11/11 00:00
MHDA- 2019/11/11 00:01
CRDT- 2021/01/06 17:10
PHST- 2021/01/06 17:10 [entrez]
PHST- 2019/11/11 00:00 [pubmed]
PHST- 2019/11/11 00:01 [medline]
AID - 10.1021/acsbiomaterials.9b01073 [doi]
PST - ppublish
SO  - ACS Biomater Sci Eng. 2019 Nov 11;5(11):6274-6280. doi: 
      10.1021/acsbiomaterials.9b01073. Epub 2019 Oct 25.