PMID- 33405657
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210106
IS  - 2373-9878 (Electronic)
IS  - 2373-9878 (Linking)
VI  - 5
IP  - 6
DP  - 2019 Jun 10
TI  - Fluorogenic Probe for Detecting Active Matrix Metalloproteinase-3 (MMP-3) in 
      Plasma and Peripheral Blood Neutrophils to Indicate the Severity of Rheumatoid 
      Arthritis.
PG  - 3039-3048
LID - 10.1021/acsbiomaterials.9b00084 [doi]
AB  - Diagnosis of patients with rheumatoid arthritis (RA) is essential for early and 
      accurate drug treatment to protect the patient from joint bone erosion and 
      relieve symptoms of the disease. In some cases, the RA patient's X-ray images and 
      other clinical diagnostic methods are often difficult to distinguish from 
      different diseases, such as gout, osteoarthritis, and other inflammatory 
      conditions. Thus, methods for diagnosis of disease activity and real-time 
      monitoring of therapeutic effect and accurate differentiation from other bone 
      diseases are needed. In this article, we suggest a matrix metalloproteinase-3 
      (MMP-3)-specific protease-activated probe immobilized in vitro kit and cell 
      staining for flow cytometry analysis as methods to support clinical diagnosis. To 
      overcome interindividual differences, we used phorbol 12-myristate 13-acetate 
      (PMA)-activated plasma from 269 RA patients, 49 osteoarthritis patients, and 30 
      healthy volunteers. The in vitro kit developed for PMA-activated plasma showed 
      potential for identifying disease severity and distinguishing RA from other bone 
      diseases. In particular, expression of active MMP-3 increased until the moderate 
      disease activity and then sharply decreased at severe disease. We suggest an 
      analysis of intracellular MMP-3-specific protease-activated probe staining by 
      flow cytometry. Compared with anti-MMP-3 antibody staining, the results for 
      active MMP-3 in neutrophils using the probe exactly matched the results obtained 
      with the in vitro kit. We also confirmed that expression of active MMP-3 was 
      mainly from neutrophils. Together, these results suggest that the MMP-3-specific 
      protease-activated probe might be a promising noninvasive tool for accurate 
      diagnosis of disease severity and differentiation from similar bone diseases as 
      well as for monitoring therapeutic efficacy.
FAU - Lee, Ruda
AU  - Lee R
AD  - International Research Organization for Advanced Science and Technology (IROAST), 
      Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto, 860-8555, Japan.
AD  - Magnesium Research Center (MRC), Kumamoto University, 2-39-1 Kurokami, Chuo-ku, 
      Kumamoto, 860-8555, Japan.
FAU - Choi, Sung-Jae
AU  - Choi SJ
AD  - Department of Internal Medicine, Division of Rheumatology, College of Medicine, 
      Korea University, 516 Danwon-gu, Ansan, 15355, Republic of Korea.
FAU - Moon, Kyung Chul
AU  - Moon KC
AD  - Department of Laboratory Medicine, College of Medicine, Korea University, 148 
      Gurodong-ro, Guro-ku, Seoul, 08308, Republic of Korea.
FAU - Park, Jong Woong
AU  - Park JW
AD  - Department of Orthopedic Surgery, College of Medicine, Korea University, 
      Anam-dong 5-ga, Seongbuk-gu, Seoul, 02841, Republic of Korea.
FAU - Kim, Kwangmeyung
AU  - Kim K
AUID- ORCID: 0000-0001-7919-188X
AD  - Biomedical Research Center, Korea Institute of Science and Technology, 5. 
      Hwarang-ro 14-gil, Seongbuk-gu, Seoul 02792, Republic of Korea.
FAU - Yoon, Soo-Young
AU  - Yoon SY
AD  - Department of Laboratory Medicine, College of Medicine, Korea University, 148 
      Gurodong-ro, Guro-ku, Seoul, 08308, Republic of Korea.
FAU - Youn, Inchan
AU  - Youn I
AUID- ORCID: 0000-0002-0977-0808
AD  - Biomedical Research Center, Korea Institute of Science and Technology, 5. 
      Hwarang-ro 14-gil, Seongbuk-gu, Seoul 02792, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20190528
PL  - United States
TA  - ACS Biomater Sci Eng
JT  - ACS biomaterials science & engineering
JID - 101654670
SB  - IM
OTO - NOTNLM
OT  - blood cell activation
OT  - matrix metalloproteinase-3
OT  - neutrophils
OT  - protease-activated sensor
OT  - rheumatoid arthritis
EDAT- 2019/06/10 00:00
MHDA- 2019/06/10 00:01
CRDT- 2021/01/06 17:10
PHST- 2021/01/06 17:10 [entrez]
PHST- 2019/06/10 00:00 [pubmed]
PHST- 2019/06/10 00:01 [medline]
AID - 10.1021/acsbiomaterials.9b00084 [doi]
PST - ppublish
SO  - ACS Biomater Sci Eng. 2019 Jun 10;5(6):3039-3048. doi: 
      10.1021/acsbiomaterials.9b00084. Epub 2019 May 28.