PMID- 33406818
OWN - NLM
STAT- MEDLINE
DCOM- 20210302
LR  - 20210302
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 13
IP  - 3
DP  - 2021 Jan 27
TI  - Ibandronate-Loaded Carbon Nanohorns Fabricated Using Calcium Phosphates as 
      Mediators and Their Effects on Macrophages and Osteoclasts.
PG  - 3701-3712
LID - 10.1021/acsami.0c20923 [doi]
AB  - Carbon nanohorns (CNHs), a type of nanocarbon, have been studied for the 
      application of drug delivery systems (DDSs) because they are easily 
      functionalized, support bone regeneration, can be used to perform 
      photohyperthermia, have low toxicity, and are easily phagocytosed by macrophages. 
      To take advantage of these features of CNHs, we developed a DDS for the local 
      treatment of bone metastasis by loading the antibone resorption drug ibandronate 
      (IBN) onto CNHs. The poor adsorption of IBN onto CNHs due to the weak 
      hydrophilic-hydrophobic interaction was overcome by using calcium phosphates 
      (CaPs) as mediators. In the fabrication process, we used oxidized CNH (OxCNH), 
      which is less hydrophobic, onto which IBN was coprecipitated with CaP from a 
      labile supersaturated CaP solution. OxCNH-CaP-IBN composite nanoparticles exerted 
      stronger cell-suppressive effects than OxCNH and IBN in both murine macrophages 
      (RAW264.7 cells) and osteoclasts (differentiated from RAW264.7 cells). 
      OxCNH-CaP-IBN composite nanoparticles were efficiently phagocytosed by macrophage 
      cells, where they specifically accumulated in lysosomes. The stronger 
      cell-suppressive effects were likely due to intracellular delivery of IBN, i.e., 
      the release of IBN from OxCNH-CaP-IBN composite nanoparticles via dissociation of 
      CaP in the acidic environment of lysosomes. Our findings suggest that 
      OxCNH-CaP-IBN composite nanoparticles are potentially useful for the local 
      treatment of metastatic bone destruction.
FAU - Nakamura, Maki
AU  - Nakamura M
AUID- ORCID: 0000-0002-6759-3205
AD  - Nanomaterials Research Institute, National Institute of Advanced Industrial 
      Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 
      305-8565, Japan.
FAU - Ueda, Katsuya
AU  - Ueda K
AD  - Biomedical Engineering Division, Graduate School of Medicine, Science and 
      Technology, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
FAU - Yamamoto, Yumiko
AU  - Yamamoto Y
AD  - Nanomaterials Research Institute, National Institute of Advanced Industrial 
      Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 
      305-8565, Japan.
FAU - Aoki, Kaoru
AU  - Aoki K
AD  - Physical Therapy Division, School of Health Sciences, Shinshu University, 3-1-1 
      Asahi, Matsumoto, Nagano 390-8621, Japan.
FAU - Zhang, Minfang
AU  - Zhang M
AUID- ORCID: 0000-0002-3983-3355
AD  - CNT Application Research Center, National Institute of Advanced Industrial 
      Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 
      305-8565, Japan.
FAU - Saito, Naoto
AU  - Saito N
AD  - Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge 
      Research, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
FAU - Yudasaka, Masako
AU  - Yudasaka M
AD  - Nanomaterials Research Institute, National Institute of Advanced Industrial 
      Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 
      305-8565, Japan.
AD  - Faculty of Science & Technology, Meijo University, 1-501 Shiogamaguchi, 
      Tenpaku-ku, Nagoya, Aichi 468-8502, Japan.
LA  - eng
PT  - Journal Article
DEP - 20210106
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Bone Density Conservation Agents)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Drug Carriers)
RN  - 0 (Nanotubes, Carbon)
RN  - UMD7G2653W (Ibandronic Acid)
SB  - IM
MH  - Animals
MH  - Bone Density Conservation Agents/administration & dosage/*pharmacology
MH  - Calcium Phosphates/chemistry
MH  - Drug Carriers/*chemistry
MH  - Ibandronic Acid/administration & dosage/*pharmacology
MH  - Macrophages/*drug effects
MH  - Mice
MH  - Nanotubes, Carbon/*chemistry/ultrastructure
MH  - Osteoclasts/*drug effects
MH  - RAW 264.7 Cells
OTO - NOTNLM
OT  - calcium phosphate
OT  - carbon nanohorns
OT  - ibandronate
OT  - macrophage
OT  - osteoclast
EDAT- 2021/01/08 06:00
MHDA- 2021/03/03 06:00
CRDT- 2021/01/07 05:40
PHST- 2021/01/08 06:00 [pubmed]
PHST- 2021/03/03 06:00 [medline]
PHST- 2021/01/07 05:40 [entrez]
AID - 10.1021/acsami.0c20923 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2021 Jan 27;13(3):3701-3712. doi: 
      10.1021/acsami.0c20923. Epub 2021 Jan 6.