PMID- 33406852
OWN - NLM
STAT- MEDLINE
DCOM- 20211112
LR  - 20211112
IS  - 0031-949X (Print)
IS  - 0031-949X (Linking)
VI  - 111
IP  - 8
DP  - 2021 Aug
TI  - Overexpression of Solanum tuberosum Respiratory Burst Oxidase Homolog A (StRbohA) 
      Promotes Potato Tolerance to Phytophthora infestans.
PG  - 1410-1419
LID - 10.1094/PHYTO-10-20-0482-R [doi]
AB  - Reactive oxygen species (ROSs) represent one of the first lines of plants' 
      biochemical defense against pathogens. Plants' respiratory burst oxidase homologs 
      (RBOHs) produce ROSs as byproducts in several cellular compartments. In potato 
      tubers, Solanum tuberosum respiratory burst oxidase homolog (StRBOHs) are 
      involved in suberization and healing of wounded tissues. StRbohA has been tested 
      in the model plant Arabidopsis thaliana, which led to enhanced plant defense 
      against the soilborne pathogen Verticillium dahliae. Here, we showed that 
      overexpressing StRbohA in potato plants increases plant tolerance to the oomycete 
      Phytophthora infestans, the causal agent of late blight disease. Transgenic 
      potato plants expressing StRbohA showed reduced disease symptoms (necrosis) 
      compared with the wild type. In parallel, the expression of pathogenesis-related 
      genes (PRs); RBOHs; antioxidation-related genes CPRX1, PRX2, APRX1, CAT1, and 
      CAT2; and genes involved in the biosynthesis pathways of jasmonic and salicylic 
      acids (ICS, PAL1, PAL2, LOX1, LOX2, and LOX3) exhibited significant increases in 
      transgenic plants in response to infection. After higher expression of RBOHs, 
      ROSs accumulated more in inoculation sites of the transgenic plants. ROSs act as 
      signals that activate gene expression in the salicylic acid (SA) biosynthesis 
      pathway, leading to the accumulation of SA and triggering SA-based defense 
      mechanisms. SA-responsive PRs showed higher expression in the transgenic plants, 
      which resulted in the restriction of pathogen growth in plant tissues. These 
      results demonstrate the effective role of StRbohA in increasing potato defense 
      against P. infestans.
FAU - Soliman, Atta
AU  - Soliman A
AD  - Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, 
      Canada.
AD  - Department of Genetics, Faculty of Agriculture, University of Tanta, Tanta 31111, 
      Egypt.
AD  - Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, 
      Lethbridge, AB T1J 4B1, Canada.
FAU - Adam, Lorne R
AU  - Adam LR
AD  - Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, 
      Canada.
FAU - Rehal, Pawanpuneet K
AU  - Rehal PK
AD  - Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, 
      Canada.
FAU - Daayf, Fouad
AU  - Daayf F
AUID- ORCID: 0000-0001-8912-0460
AD  - Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, 
      Canada.
LA  - eng
PT  - Journal Article
DEP - 20210922
PL  - United States
TA  - Phytopathology
JT  - Phytopathology
JID - 9427222
RN  - 0 (Plant Proteins)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 1.6.99.- (superoxide-forming enzyme)
RN  - Verticillium dahliae
SB  - IM
MH  - Ascomycota
MH  - NADPH Oxidases
MH  - *Phytophthora infestans
MH  - Plant Diseases
MH  - Plant Proteins/genetics
MH  - Plants, Genetically Modified
MH  - *Solanum tuberosum/genetics
OTO - NOTNLM
OT  - P. infestans
OT  - Solanum
OT  - StRbohA
OT  - disease control and pest management
OT  - disease resistance
OT  - fungal pathogens
OT  - host-parasite interactions
OT  - late blight
OT  - oomycetes
OT  - plant immune responses
OT  - transgenic plants
EDAT- 2021/01/08 06:00
MHDA- 2021/11/16 06:00
CRDT- 2021/01/07 05:40
PHST- 2021/01/08 06:00 [pubmed]
PHST- 2021/11/16 06:00 [medline]
PHST- 2021/01/07 05:40 [entrez]
AID - 10.1094/PHYTO-10-20-0482-R [doi]
PST - ppublish
SO  - Phytopathology. 2021 Aug;111(8):1410-1419. doi: 10.1094/PHYTO-10-20-0482-R. Epub 
      2021 Sep 22.