PMID- 33406991
OWN - NLM
STAT- MEDLINE
DCOM- 20211206
LR  - 20211214
IS  - 1555-8584 (Electronic)
IS  - 1547-6286 (Print)
IS  - 1547-6286 (Linking)
VI  - 18
IP  - 5
DP  - 2021 May
TI  - Dengue virus strain 2 capsid protein switches the annealing pathway and reduces 
      intrinsic dynamics of the conserved 5' untranslated region.
PG  - 718-731
LID - 10.1080/15476286.2020.1860581 [doi]
AB  - The capsid protein of dengue virus strain 2 (DENV2C) promotes nucleic acid 
      structural rearrangements using chaperone activity. However, the role of DENV2C 
      during the interaction of RNA elements in the conserved 5' untranslated region 
      (5'UTR) to the 3' untranslated region (3'UTR) is still unclear. Thus, we 
      investigated the effect of DENV2C on the annealing mechanism of two RNA hairpin 
      elements from the 5'UTR to their complementary sequences during (+)/(-) 
      ds-RNAformation and (+) RNA circularization. DENV2C was found to switch the 
      annealing pathway for RNA elements involved in (+)/(-) ds-RNA formation, but not 
      for RNA elements related to (+) RNA circularization. In addition, we also 
      determined that DENV2C modulates intrinsic dynamics and reduces kinetically 
      trapped unfavourable conformations of the 5'UTR sequence. Thus, our results 
      provide mechanistic insights by which DENV2C chaperones the interactions between 
      RNA elements at the 5' and 3' ends during genome recombination, a prerequisite 
      for DENV replication.
FAU - Yong, Xin Ee
AU  - Yong XE
AUID- ORCID: 0000-0002-4235-0071
AD  - NUS Graduate School for integrative Sciences and Engineering Programme, National 
      University of Singapore, Singapore.
AD  - Centre for Bioimaging Sciences, National University of Singapore, Singapore, 
      Singapore.
FAU - Raghuvamsi, Palur Venkata
AU  - Raghuvamsi PV
AD  - Department of Biological Sciences, National University of Singapore, Singapore, 
      Singapore.
FAU - Anand, Ganesh S
AU  - Anand GS
AD  - Department of Biological Sciences, National University of Singapore, Singapore, 
      Singapore.
FAU - Wohland, Thorsten
AU  - Wohland T
AD  - Centre for Bioimaging Sciences, National University of Singapore, Singapore, 
      Singapore.
AD  - Department of Biological Sciences, National University of Singapore, Singapore, 
      Singapore.
FAU - Sharma, Kamal K
AU  - Sharma KK
AD  - Centre for Bioimaging Sciences, National University of Singapore, Singapore, 
      Singapore.
AD  - Department of Biological Sciences, National University of Singapore, Singapore, 
      Singapore.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210107
PL  - United States
TA  - RNA Biol
JT  - RNA biology
JID - 101235328
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Capsid Proteins)
RN  - 0 (RNA, Circular)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - 5' Untranslated Regions/*genetics
MH  - Base Pairing/genetics
MH  - Base Sequence
MH  - Capsid Proteins/metabolism/*physiology
MH  - Conserved Sequence
MH  - Dengue Virus/genetics/*metabolism/physiology
MH  - Genome, Viral/physiology
MH  - Nucleic Acid Conformation
MH  - Protein Biosynthesis/genetics
MH  - RNA, Circular/chemistry/genetics
MH  - RNA, Viral/chemistry/genetics
MH  - Virus Replication/genetics
PMC - PMC8078513
OTO - NOTNLM
OT  - FRET-FCS
OT  - RNA chaperone
OT  - RNA replication
OT  - genome circularization
OT  - kissing loop
COIS- The authors declare no conflict of interest.
EDAT- 2021/01/08 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/01/07
CRDT- 2021/01/07 05:41
PHST- 2021/01/08 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/01/07 05:41 [entrez]
PHST- 2021/01/07 00:00 [pmc-release]
AID - 1860581 [pii]
AID - 10.1080/15476286.2020.1860581 [doi]
PST - ppublish
SO  - RNA Biol. 2021 May;18(5):718-731. doi: 10.1080/15476286.2020.1860581. Epub 2021 
      Jan 7.