PMID- 33407514
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210111
IS  - 1475-2867 (Print)
IS  - 1475-2867 (Electronic)
IS  - 1475-2867 (Linking)
VI  - 21
IP  - 1
DP  - 2021 Jan 6
TI  - Circ-CCDC66 upregulates REXO1 expression to aggravate cervical cancer progression 
      via restraining miR-452-5p.
PG  - 20
LID - 10.1186/s12935-020-01732-8 [doi]
LID - 20
AB  - BACKGROUND: Cervical cancer is one most common cancer types among females over 
      the world. While its underlying mechanisms remain unclear. Circ-CCDC66 has been 
      revealed to participate in multiple biological functions, and contribute to 
      various diseases' progression. In the current study, we aimed to demonstrate the 
      role of circ-CCDC66 in cervical cancer progression. METHODS: Real-time 
      quantitative PCR (RT-qPCR) was conducted to measure the expression of 
      circ-CCDC66, miR-452-5p, and REXO1 mRNA. Cell fractionation assay and RNA 
      fluorescence in situ hybridization (FISH) were performed to locate circ-CCDC66 in 
      cells. Cell account kit 8 (CCK-8) was used to detect cell proliferation ability. 
      Transwell assay was applied to evaluate cell migration or invasion ability. 
      Bioinformatics analysis, biotinylated RNA pull-down, RNA immunoprecipitation, and 
      dual-luciferase reporter assays were conducted to assess the association between 
      miR-452 and circ-CCDC66 or REXO1. Western blot was applied to measure the protein 
      expression of REXO1. The animal tumor model was used to assess the effect of 
      circ-CCDC66 in vivo. RESULTS: The expression of circ-CCDC66 was upregulated in 
      cervical cancer tumor tissues in comparison with normal tissues, and correlated 
      with later tumor stage and larger tumor size. Downregulated circ-CCDC66 inhibited 
      cervical cancer cell proliferation, migration, and invasion. Circ-CCDC66 was an 
      efficient molecular sponge for miR-452-5p, and negatively regulated miR-452-5p 
      expression. MiR-452-5p directly targeted to REXO1. Circ-CCDC66 regulated REXO1 
      expression to modulate cervical cancer progression via miR-452-5p. Moreover, 
      downregulated circ-CCDC66 was found to suppress tumor growth in vivo. CONCLUSION: 
      Our results demonstrated the role of circ-CCDC66/miR-452-5p/REXO1 axis in 
      cervical cancer progression, we might provide novel therapeutic targets for 
      cervical cancer clinical intervention.
FAU - Zhang, Yan
AU  - Zhang Y
AUID- ORCID: 0000-0001-7798-531X
AD  - Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, 
      Wuhan, China. Doctor8118@163.com.
FAU - Li, Xing
AU  - Li X
AD  - Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, 
      Wuhan, China.
FAU - Zhang, Jun
AU  - Zhang J
AD  - Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, 
      Wuhan, China.
FAU - Mao, Lin
AU  - Mao L
AD  - Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, 
      Wuhan, China.
LA  - eng
PT  - Journal Article
DEP - 20210106
PL  - England
TA  - Cancer Cell Int
JT  - Cancer cell international
JID - 101139795
PMC - PMC7789749
OTO - NOTNLM
OT  - Cervical cancer
OT  - Circ-CCDC66
OT  - Circular RNA
OT  - REXO1
OT  - miR-452-5p
COIS- There is no conflict of interests to declare in the current study.
EDAT- 2021/01/08 06:00
MHDA- 2021/01/08 06:01
PMCR- 2021/01/06
CRDT- 2021/01/07 05:47
PHST- 2020/08/26 00:00 [received]
PHST- 2020/12/21 00:00 [accepted]
PHST- 2021/01/07 05:47 [entrez]
PHST- 2021/01/08 06:00 [pubmed]
PHST- 2021/01/08 06:01 [medline]
PHST- 2021/01/06 00:00 [pmc-release]
AID - 10.1186/s12935-020-01732-8 [pii]
AID - 1732 [pii]
AID - 10.1186/s12935-020-01732-8 [doi]
PST - epublish
SO  - Cancer Cell Int. 2021 Jan 6;21(1):20. doi: 10.1186/s12935-020-01732-8.