PMID- 33407892
OWN - NLM
STAT- MEDLINE
DCOM- 20210121
LR  - 20210121
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 52
IP  - 1
DP  - 2021 Jan 6
TI  - Protection studies of an excretory-secretory protein HcABHD against Haemonchus 
      contortus infection.
PG  - 3
LID - 10.1186/s13567-020-00871-0 [doi]
LID - 3
AB  - Unlike the successful immunization of native H. contortus antigens that 
      contributed to the realization of the first commercial vaccine Barbervax, not 
      many studies revealed the encouraging protective efficacies of recombinant H. 
      contortus antigens in laboratory trials or under field conditions. In our 
      preliminary study, H. contortus alpha/beta-hydrolase domain protein (HcABHD) was 
      demonstrated to be an immunomodulatory excretory-secretory (ES) protein that 
      interacts with goat T cells. We herein evaluated the protective capacities of two 
      HcABHD preparations, recombinant HcABHD (rHcABHD) antigen and anti-rHcABHD IgG, 
      against H. contortus challenge via active and passive immunization trials, 
      respectively. Parasitological parameter, antibody responses, hematological 
      pathology and cytokine profiling in unchallenged and challenged goats were 
      monitored and determined throughout both trials. Subcutaneous administration of 
      rHcABHD with Freund adjuvants elicited protective immune responses in challenged 
      goats, diminishing cumulative fecal egg counts (FEC) and total worm burden by 
      54.0% and 74.2%, respectively, whereas passive immunization with anti-rHcABHD IgG 
      conferred substantial protection to challenged goats by generating a 51.5% 
      reduction of cumulative FEC and a 73.8% reduction of total worm burden. 
      Additionally, comparable changes of mucosal IgA levels, circulating IgG levels, 
      hemoglobin levels, and serum interleukin (IL)-4 and IL-17A levels were observed 
      in rHcABHD protein/anti-rHcABHD IgG immunized goats in both trials. Taken 
      together, the recombinant version of HcABHD might have further application under 
      field conditions in protecting goats against H. contortus infection, and the 
      integrated immunological pipeline of ES antigen identification, screening and 
      characterization may provide new clues for further development of recombinant 
      subunit vaccines to control H. contortus.
FAU - Lu, Mingmin
AU  - Lu M
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, 
      Jiangsu, P. R. China.
FAU - Tian, Xiaowei
AU  - Tian X
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, 
      Jiangsu, P. R. China.
FAU - Zhang, Yang
AU  - Zhang Y
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, 
      Jiangsu, P. R. China.
FAU - Wang, Wenjuan
AU  - Wang W
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, 
      Jiangsu, P. R. China.
FAU - Tian, Ai-Ling
AU  - Tian AL
AD  - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, 
      Gansu, P. R. China.
FAU - Aimulajiang, Kalibixiati
AU  - Aimulajiang K
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, 
      Jiangsu, P. R. China.
FAU - Liu, Lianrui
AU  - Liu L
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, 
      Jiangsu, P. R. China.
FAU - Li, Charles
AU  - Li C
AD  - Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research 
      Center, Agricultural Research Service, U.S. Department of Agriculture, 
      Beltsville, MD, 20705, USA.
FAU - Yan, Ruofeng
AU  - Yan R
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, 
      Jiangsu, P. R. China.
FAU - Xu, Lixin
AU  - Xu L
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, 
      Jiangsu, P. R. China.
FAU - Song, Xiaokai
AU  - Song X
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, 
      Jiangsu, P. R. China.
FAU - Li, Xiangrui
AU  - Li X
AUID- ORCID: 0000-0003-3538-8837
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, 
      Jiangsu, P. R. China. lixiangrui@njau.edu.cn.
LA  - eng
GR  - 2017YFD0501200/National Key Research and Development Program of China/
GR  - BZ2019013/Policy Guidance Project of Jiangsu Province for International 
      Scientific and Technological Cooperation/
GR  - 2015CB150300/National Key Basic Research Program (973 Program)/
PT  - Journal Article
PT  - Randomized Controlled Trial, Veterinary
DEP - 20210106
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Antigens, Helminth)
RN  - 0 (Helminth Proteins)
RN  - 0 (Vaccines)
RN  - 0 (Vaccines, Synthetic)
SB  - IM
MH  - Animals
MH  - Antigens, Helminth/therapeutic use
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Goat Diseases/*parasitology/prevention & control
MH  - Goats
MH  - Haemonchiasis/prevention & control/*veterinary
MH  - Haemonchus/*immunology
MH  - Helminth Proteins/*therapeutic use
MH  - Male
MH  - Parasite Egg Count/veterinary
MH  - Vaccines/*therapeutic use
MH  - Vaccines, Synthetic/therapeutic use
PMC - PMC7786147
OTO - NOTNLM
OT  - H. contortus
OT  - excretory-secretory protein
OT  - goats
OT  - immunization
OT  - Alpha/beta-hydrolase
COIS- The authors declare that they have no competing interests.
EDAT- 2021/01/08 06:00
MHDA- 2021/01/22 06:00
PMCR- 2021/01/06
CRDT- 2021/01/07 05:51
PHST- 2020/08/07 00:00 [received]
PHST- 2020/11/19 00:00 [accepted]
PHST- 2021/01/07 05:51 [entrez]
PHST- 2021/01/08 06:00 [pubmed]
PHST- 2021/01/22 06:00 [medline]
PHST- 2021/01/06 00:00 [pmc-release]
AID - 10.1186/s13567-020-00871-0 [pii]
AID - 871 [pii]
AID - 10.1186/s13567-020-00871-0 [doi]
PST - epublish
SO  - Vet Res. 2021 Jan 6;52(1):3. doi: 10.1186/s13567-020-00871-0.