PMID- 33409934
OWN - NLM
STAT- MEDLINE
DCOM- 20210818
LR  - 20210818
IS  - 2190-3883 (Electronic)
IS  - 1234-1983 (Linking)
VI  - 62
IP  - 2
DP  - 2021 May
TI  - Cytomolecular analysis of mutants, breeding lines, and varieties of camelina 
      (Camelina sativa L. Crantz).
PG  - 199-205
LID - 10.1007/s13353-020-00600-5 [doi]
AB  - Camelina sativa L. Crantz (Brassicaceae family), known as camelina, has gained 
      new attention as a re-emerging oil seed crop. With a unique seed oil profile, 
      with the majority of the fatty acids consisting of linolenic (C18:3), oleic 
      (C18:1), linoleic (C18:2), and eicosenoic (C20:1), camelina oil is reported to be 
      useful as a food oil and biofuel. However, there are still many unknown factors 
      about the structure and genetic variability of this crop. Chromosomal 
      localization of ribosomal DNA was performed using fluorescence in situ 
      hybridization (FISH) with 5S rDNA and 25S rDNA sequences as molecular probes on 
      mitotic chromosomes of enzymatically digested root-tip meristematic cells. Here, 
      we present for the first time a comparative analysis of selected genotypes 
      (cultivars, breeding lines and mutants) of C. sativa with the use of cytogenetic 
      techniques. The main aim of the study was to determine the intraspecific and 
      interspecific polymorphisms in the structure of chromosomes of selected 
      accessions using conserved 5S and 25S rDNA repetitive sequences as molecular 
      probes. The results were compared with C. microcarpa (closely related to C. 
      sativa) rDNA gene loci distribution. The presence of minor rDNA sites was 
      discussed and compared with other Brassicaceae species. In addition, 
      demonstration karyograms of C. sativa and C. microcarpa mapped with rDNA probes 
      were prepared based on the cv. "Przybrodzka" and GE2011-02 genotype, 
      respectively. The use of 5S and 25S rDNA probes provided an insight on the genome 
      structure of C. sativa at the cytogenetic level and can help to understand the 
      genome organization of this crop. The putative role of cytogenetic markers in 
      phylogenetic analyses of camelina was discussed, as well.
FAU - Kwiatek, Michal T
AU  - Kwiatek MT
AUID- ORCID: 0000-0001-9442-3124
AD  - Department of Genetics and Plant Breeding, Faculty of Agronomy and 
      Bioengineering, Poznan University of Life Sciences, Dojazd 11Str., 60-632, 
      Poznan, Poland. michal.kwiatek@up.poznan.pl.
FAU - Drozdowska, Zofia
AU  - Drozdowska Z
AD  - Department of Genetics and Plant Breeding, Faculty of Agronomy and 
      Bioengineering, Poznan University of Life Sciences, Dojazd 11Str., 60-632, 
      Poznan, Poland.
FAU - Kurasiak-Popowska, Danuta
AU  - Kurasiak-Popowska D
AUID- ORCID: 0000-0002-2214-406X
AD  - Department of Genetics and Plant Breeding, Faculty of Agronomy and 
      Bioengineering, Poznan University of Life Sciences, Dojazd 11Str., 60-632, 
      Poznan, Poland.
FAU - Noweiska, Aleksandra
AU  - Noweiska A
AD  - Department of Genetics and Plant Breeding, Faculty of Agronomy and 
      Bioengineering, Poznan University of Life Sciences, Dojazd 11Str., 60-632, 
      Poznan, Poland.
FAU - Nawracala, Jerzy
AU  - Nawracala J
AUID- ORCID: 0000-0002-6824-156X
AD  - Department of Genetics and Plant Breeding, Faculty of Agronomy and 
      Bioengineering, Poznan University of Life Sciences, Dojazd 11Str., 60-632, 
      Poznan, Poland.
LA  - eng
GR  - 005/RID/2018/19/Ministerstwo Nauki i Szkolnictwa Wyzszego/
PT  - Journal Article
DEP - 20210107
PL  - England
TA  - J Appl Genet
JT  - Journal of applied genetics
JID - 9514582
RN  - 0 (DNA Probes)
RN  - 0 (Plant Oils)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 5S)
RN  - 130527-23-0 (RNA, ribosomal, 25S)
SB  - IM
MH  - *Brassicaceae/genetics
MH  - DNA Probes
MH  - Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Phylogeny
MH  - *Plant Breeding
MH  - Plant Oils
MH  - RNA, Ribosomal/genetics
MH  - RNA, Ribosomal, 5S/genetics
OTO - NOTNLM
OT  - Camelina sativa
OT  - Fluorescence in situ hybridization
OT  - Karyogram
OT  - Molecular cytogenetics
OT  - rDNA gene loci
EDAT- 2021/01/08 06:00
MHDA- 2021/08/19 06:00
CRDT- 2021/01/07 06:13
PHST- 2020/09/18 00:00 [received]
PHST- 2020/11/26 00:00 [accepted]
PHST- 2020/11/21 00:00 [revised]
PHST- 2021/01/08 06:00 [pubmed]
PHST- 2021/08/19 06:00 [medline]
PHST- 2021/01/07 06:13 [entrez]
AID - 10.1007/s13353-020-00600-5 [pii]
AID - 10.1007/s13353-020-00600-5 [doi]
PST - ppublish
SO  - J Appl Genet. 2021 May;62(2):199-205. doi: 10.1007/s13353-020-00600-5. Epub 2021 
      Jan 7.