PMID- 33412574
OWN - NLM
STAT- MEDLINE
DCOM- 20210511
LR  - 20210511
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 16
IP  - 1
DP  - 2021
TI  - Interleukin-18 and interferon-gamma single nucleotide polymorphisms in Egyptian 
      patients with tuberculosis.
PG  - e0244949
LID - 10.1371/journal.pone.0244949 [doi]
LID - e0244949
AB  - BACKGROUND: Interleukin-18 (IL-18) and interferon-gamma (IFN-gamma) are cytokines of 
      crucial role in inflammation and immune reactions. There is a growing evidence 
      supporting important roles for IL-18 and IFN gamma in tuberculosis (TB) infection and 
      anti-tuberculosis immunity. OBJECTIVE: To evaluate the role of polymorphisms in 
      IL-18-607 and -137 and INF-gamma +874 in susceptibility to TB infection among 
      Egyptian patients. METHODS: A case control study was conducted to investigate the 
      polymorphism at IL-18-607, -137 and INF-gamma+874 by sequence specific 
      primer-polymerase chain reaction (SSP- PCR) in 105 patients with pulmonary and 
      extra pulmonary tuberculosis and 106 controls. RESULTS: A significant protective 
      effect against TB was found in homozygous CC genotype at IL-18 -137G/C, in 
      addition to a 7-fold risk with GG and GC genotypes in the recessive model. Apart 
      from a decreased risk with the AC genotype, no association was detected between 
      the susceptibility to TB and different genotypes or alleles at the IL-18 -607A/C 
      site. The homozygous AA genotype in INF-gamma+874 showed a significant higher risk to 
      TB than the homozygous TT or heterozygous AT genotypes with nearly a 2-fold risk 
      of TB infection with the A allele. Regarding haplotype association, the GC 
      haplotype was strongly associated with TB infection compared to other haplotypes. 
      CONCLUSION: These findings suggest; for the first time in Egypt; a significant 
      risk to TB infection with SNP at the IL-18-137G/C with no LD with SNP at the 
      IL-18-607 site. The homozygous AA genotype in INF-gamma+874 showed a significant 
      higher risk to TB than the homozygous TT or heterozygous AT genotypes.
FAU - Hassuna, Noha A
AU  - Hassuna NA
AUID- ORCID: 0000-0001-6702-2341
AD  - Department of Medical Microbiology and Immunology, Faculty of Medicine, Minia 
      University, Minia, Egypt.
FAU - El Feky, Mohamed
AU  - El Feky M
AD  - Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut 
      University, Assiut, Egypt.
FAU - Hussein, Aliae A R Mohamed
AU  - Hussein AARM
AD  - Department of Chest Diseases and Tuberculosis, Faculty of Medicine, Assiut 
      University, Assiut, Egypt.
FAU - Mahmoud, Manal A
AU  - Mahmoud MA
AD  - Department of Chest Diseases and Tuberculosis, Faculty of Medicine, Assiut 
      University, Assiut, Egypt.
FAU - Idriss, Naglaa K
AU  - Idriss NK
AD  - Department of Medical Biochemistry, Faculty of Medicine, Assiut University, 
      Assiut, Egypt.
FAU - Abdelwahab, Sayed F
AU  - Abdelwahab SF
AUID- ORCID: 0000-0002-9636-7485
AD  - Department of Medical Microbiology and Immunology, Faculty of Medicine, Minia 
      University, Minia, Egypt.
AD  - Division of Pharmaceutical Microbiology, Department of Pharmaceutics and 
      Industrial Pharmacy, Taif College of Pharmacy, Taif University, Taif, Saudi 
      Arabia.
FAU - Ibrahim, Maggie A
AU  - Ibrahim MA
AD  - Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut 
      University, Assiut, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20210107
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antitubercular Agents)
RN  - 0 (Interleukin-18)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Alleles
MH  - Antitubercular Agents/therapeutic use
MH  - Case-Control Studies
MH  - Egypt
MH  - Female
MH  - Gene Frequency
MH  - *Genetic Predisposition to Disease
MH  - *Genotype
MH  - Humans
MH  - Interferon-gamma/*genetics
MH  - Interleukin-18/*genetics
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Single Nucleotide
MH  - Tuberculosis/drug therapy/*genetics
MH  - Young Adult
PMC - PMC7790531
COIS- The authors have declared that no competing interests exist.
EDAT- 2021/01/08 06:00
MHDA- 2021/05/12 06:00
PMCR- 2021/01/07
CRDT- 2021/01/07 20:19
PHST- 2020/08/31 00:00 [received]
PHST- 2020/12/19 00:00 [accepted]
PHST- 2021/01/07 20:19 [entrez]
PHST- 2021/01/08 06:00 [pubmed]
PHST- 2021/05/12 06:00 [medline]
PHST- 2021/01/07 00:00 [pmc-release]
AID - PONE-D-20-27317 [pii]
AID - 10.1371/journal.pone.0244949 [doi]
PST - epublish
SO  - PLoS One. 2021 Jan 7;16(1):e0244949. doi: 10.1371/journal.pone.0244949. 
      eCollection 2021.