PMID- 33413513
OWN - NLM
STAT- MEDLINE
DCOM- 20211102
LR  - 20240501
IS  - 2045-8118 (Electronic)
IS  - 2045-8118 (Linking)
VI  - 18
IP  - 1
DP  - 2021 Jan 7
TI  - Structural disruption of the blood-brain barrier in repetitive primary blast 
      injury.
PG  - 2
LID - 10.1186/s12987-020-00231-2 [doi]
LID - 2
AB  - BACKGROUND: Blast-induced traumatic brain injury (bTBI) is a growing health 
      concern due to the increased use of low-cost improvised explosive devices in 
      modern warfare. Mild blast exposures are common amongst military personnel; 
      however, these women and men typically do not have adequate recovery time from 
      their injuries due to the transient nature of behavioral symptoms. bTBI has been 
      linked to heterogeneous neuropathology, including brain edema, neuronal 
      degeneration and cognitive abnormalities depending on the intensity of blast 
      overpressure and frequency. Recent studies have reported heterogeneity in 
      blood-brain barrier (BBB) permeability following blast injury. There still 
      remains a limited understanding of the pathologic changes in the BBB following 
      primary blast injuries. In this study, our goal was to elucidate the pathologic 
      pattern of BBB damage through structural analysis following single and repetitive 
      blast injury using a clinically relevant rat model of bTBI. METHODS: A validated, 
      open-ended shock tube model was used to deliver single or repetitive primary 
      blast waves. The pathology of the BBB was assessed using immunofluorescence and 
      immunohistochemistry assays. All data were analyzed using the one-way ANOVA test. 
      RESULTS: We have demonstrated that exposure to repetitive blast injury affects 
      the desmin-positive and CD13-positive subpopulations of pericytes in the BBB. 
      Changes in astrocytes and microglia were also detected. CONCLUSION: This study 
      provides analysis of the BBB components after repetitive blast injury. These 
      results will be critical as preventative and therapeutic strategies are 
      established for veterans recovering from blast-induced traumatic brain injury.
FAU - Uzunalli, Gozde
AU  - Uzunalli G
AD  - Department of Comparative Pathobiology, Purdue University College of Veterinary 
      Medicine, West Lafayette, IN, USA.
FAU - Herr, Seth
AU  - Herr S
AD  - Department of Basic Medical Sciences, Purdue University College of Veterinary 
      Medicine, West Lafayette, IN, USA.
FAU - Dieterly, Alexandra M
AU  - Dieterly AM
AD  - Department of Comparative Pathobiology, Purdue University College of Veterinary 
      Medicine, West Lafayette, IN, USA.
FAU - Shi, Riyi
AU  - Shi R
AD  - Department of Basic Medical Sciences, Purdue University College of Veterinary 
      Medicine, West Lafayette, IN, USA.
AD  - Purdue University Weldon School of Biomedical Engineering, West Lafayette, IN, 
      USA.
FAU - Lyle, L Tiffany
AU  - Lyle LT
AUID- ORCID: 0000-0003-1588-4876
AD  - Department of Comparative Pathobiology, Purdue University College of Veterinary 
      Medicine, West Lafayette, IN, USA. tiffanylyle@purdue.edu.
AD  - Center for Cancer Research, Purdue University, West Lafayette, IN, USA. 
      tiffanylyle@purdue.edu.
AD  - Center for Comparative Translational Research, Purdue University, West Lafayette, 
      IN, USA. tiffanylyle@purdue.edu.
LA  - eng
GR  - UL1 TR001108/TR/NCATS NIH HHS/United States
PT  - Journal Article
DEP - 20210107
PL  - England
TA  - Fluids Barriers CNS
JT  - Fluids and barriers of the CNS
JID - 101553157
SB  - IM
MH  - Animals
MH  - Blast Injuries/*pathology
MH  - *Blood-Brain Barrier/cytology/injuries/pathology
MH  - Brain Injuries, Traumatic/*pathology
MH  - Disease Models, Animal
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC7789532
OTO - NOTNLM
OT  - Blast-induced traumatic brain injury
OT  - Blood-brain barrier
OT  - CD13
OT  - Desmin
OT  - Neurotrauma
OT  - Pericytes
OT  - Repetitive injury
COIS- Riyi Shi is the co-founder of Neuro Vigor, a start-up company with business 
      interests of developing effective therapies for CNS neurodegenerative diseases 
      and trauma. The remaining authors declare no competing interests.
EDAT- 2021/01/09 06:00
MHDA- 2021/11/03 06:00
PMCR- 2021/01/07
CRDT- 2021/01/08 05:46
PHST- 2020/04/01 00:00 [received]
PHST- 2020/11/07 00:00 [accepted]
PHST- 2021/01/08 05:46 [entrez]
PHST- 2021/01/09 06:00 [pubmed]
PHST- 2021/11/03 06:00 [medline]
PHST- 2021/01/07 00:00 [pmc-release]
AID - 10.1186/s12987-020-00231-2 [pii]
AID - 231 [pii]
AID - 10.1186/s12987-020-00231-2 [doi]
PST - epublish
SO  - Fluids Barriers CNS. 2021 Jan 7;18(1):2. doi: 10.1186/s12987-020-00231-2.