PMID- 33413706
OWN - NLM
STAT- MEDLINE
DCOM- 20210909
LR  - 20210909
IS  - 1435-8115 (Electronic)
IS  - 1431-9276 (Linking)
VI  - 27
IP  - 2
DP  - 2021 Apr
TI  - EB1 Is Essential for Spindle Formation and Chromosome Alignment During Oocyte 
      Meiotic Maturation in Mice.
PG  - 385-391
LID - 10.1017/S1431927620024897 [doi]
AB  - The cytoskeleton plays an orchestrating role in polarized cell growth. 
      Microtubules (MTs) not only play critical roles in chromosome alignment and 
      segregation but also control cell shape, division, and motility. A member of the 
      plus-end tracking proteins, end-binding protein 1 (EB1), regulates MT dynamics 
      and plays vital roles in maintaining spindle symmetry and chromosome alignment 
      during mitosis. However, the role of EB1 in mouse oocyte meiosis remains unknown. 
      Here, we examined the localization patterns and expression levels of EB1 at 
      different stages. EB1 protein level was found to be stable during meiosis. EB1 
      mainly localized along the spindle and had a similar localization pattern as that 
      of alpha-tubulin. The EB1 protein was degraded with a Trim-Away method, and the 
      results were further confirmed with western blotting and immunofluorescence. At 
      12 h of culture after EB1 knockdown (KD), a reduced number of mature MII oocytes 
      were observed. EB1 KD led to spindle disorganization, chromosome misalignment, 
      and missegregation; beta-catenin protein binds to actin via the adherens junctional 
      complex, which was significantly reduced in the EB1 KD oocytes. Collectively, we 
      propose that the impairment of EB1 function manipulates spindle formation, 
      thereby promoting chromosomal loss, which is expected to fuel aneuploidy and 
      possibly fertilization failure.
FAU - Zhou, Dongjie
AU  - Zhou D
AUID- ORCID: 0000-0002-4065-0732
AD  - Department of Animal Science, Chungbuk National University, 356 Room, S21-5 Dong, 
      Chungdae-ro 1, Seowon-Gu, Cheongju, Chungbuk28644, South Korea.
FAU - Nie, Zheng-Wen
AU  - Nie ZW
AD  - Department of Animal Science, Chungbuk National University, 356 Room, S21-5 Dong, 
      Chungdae-ro 1, Seowon-Gu, Cheongju, Chungbuk28644, South Korea.
FAU - Cui, Xiang-Shun
AU  - Cui XS
AD  - Department of Animal Science, Chungbuk National University, 356 Room, S21-5 Dong, 
      Chungdae-ro 1, Seowon-Gu, Cheongju, Chungbuk28644, South Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Microsc Microanal
JT  - Microscopy and microanalysis : the official journal of Microscopy Society of 
      America, Microbeam Analysis Society, Microscopical Society of Canada
JID - 9712707
SB  - IM
MH  - Animals
MH  - Chromosomes
MH  - *Meiosis
MH  - Mice
MH  - Microtubules
MH  - Oocytes
MH  - *Spindle Apparatus
OTO - NOTNLM
OT  - EB1
OT  - chromosome misalignment
OT  - meiosis
OT  - oocyte
OT  - spindle formation
EDAT- 2021/01/09 06:00
MHDA- 2021/09/10 06:00
CRDT- 2021/01/08 05:48
PHST- 2021/01/09 06:00 [pubmed]
PHST- 2021/09/10 06:00 [medline]
PHST- 2021/01/08 05:48 [entrez]
AID - S1431927620024897 [pii]
AID - 10.1017/S1431927620024897 [doi]
PST - ppublish
SO  - Microsc Microanal. 2021 Apr;27(2):385-391. doi: 10.1017/S1431927620024897.