PMID- 3341371
OWN - NLM
STAT- MEDLINE
DCOM- 19880308
LR  - 20190820
IS  - 0361-8609 (Print)
IS  - 0361-8609 (Linking)
VI  - 27
IP  - 2
DP  - 1988 Feb
TI  - Divalent cations induce protofibril gelation.
PG  - 104-9
AB  - Soluble fibrin oligomers (protofibrils) undergo phase change merely by adding 1-2 
      mM Ca2+ or 25-100 microM Zn2+. The cation-induced "protofibrin" clots appear 
      similar to normally formed fibrin gels. Maximal clot turbidity of protofibrin 
      gels increases with cations in a concentration-dependent manner. Magnesium (less 
      than 0.5 mM) is ineffective in inducing protofibril gelation. Turbidity and 
      degree of polymerization (DP) [indirectly expressed as AT (activation time)] 
      appear to be positively correlated, regardless of whether the divalent cation is 
      Ca2+ or Zn2+. Cross sections of Ca2+-induced protofibrin fibers are approximately 
      6-18-fibrin-monomers-thick. With both Ca2+ and 40 microM Zn2+, fiber cross 
      section increases to 30-50 monomers thick. Negatively stained Zn2+-and 
      Ca2+-induced protofibrin gels exhibit banding periodicity of approximately 240 A, 
      similar to that of normally generated fibrin gels. Regions of lateral merging of 
      individual segments of the protofibrin leads to increased cross section of the 
      fiber and forms a branch required for gelation. These findings indicate that the 
      rate of coagulation and the ultimate thickness of the fibers both relate to 
      lateral associative processes of protofibrils, which are augmented by physiologic 
      concentrations of 2+ and Zn2+.
FAU - Marx, G
AU  - Marx G
AD  - Magen David Adom Blood Services, Tel Hashomer Medical Center, Tel Aviv, Israel.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Hematol
JT  - American journal of hematology
JID - 7610369
RN  - 0 (Biopolymers)
RN  - 0 (Cations, Divalent)
RN  - 0 (Gels)
RN  - 9001-31-4 (Fibrin)
RN  - 9001-32-5 (Fibrinogen)
RN  - 9013-56-3 (Factor XIII)
RN  - J41CSQ7QDS (Zinc)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Biopolymers
MH  - *Blood Coagulation
MH  - Calcium/*pharmacology
MH  - Cations, Divalent
MH  - Factor XIII/metabolism
MH  - *Fibrin/metabolism
MH  - Fibrinogen/metabolism
MH  - Gels
MH  - Humans
MH  - Microscopy, Electron
MH  - Nephelometry and Turbidimetry
MH  - Time Factors
MH  - Zinc/*pharmacology
EDAT- 1988/02/01 00:00
MHDA- 1988/02/01 00:01
CRDT- 1988/02/01 00:00
PHST- 1988/02/01 00:00 [pubmed]
PHST- 1988/02/01 00:01 [medline]
PHST- 1988/02/01 00:00 [entrez]
AID - 10.1002/ajh.2830270207 [doi]
PST - ppublish
SO  - Am J Hematol. 1988 Feb;27(2):104-9. doi: 10.1002/ajh.2830270207.