PMID- 33414377 OWN - NLM STAT- MEDLINE DCOM- 20210923 LR - 20220813 IS - 2044-4052 (Electronic) IS - 2044-4052 (Linking) VI - 11 IP - 1 DP - 2021 Jan 7 TI - Exercise intensity regulates cytokine and klotho responses in men. PG - 5 LID - 10.1038/s41387-020-00144-x [doi] LID - 5 AB - BACKGROUND: Short-term exercise training programs that consist of moderate intensity endurance training or high intensity interval training have become popular choices for healthy lifestyle modifications, with as little as two weeks of training being shown to improve cardiorespiratory fitness and whole-body glucose metabolism. An emerging concept in exercise biology is that exercise stimulates the release of cytokines and other factors into the blood that contribute to the beneficial effects of exercise on metabolism, but whether these factors behave similarly in response to moderate and high intensity short term training is not known. Here, we determined the effects of two short-term exercise training programs on the concentrations of select secreted cytokines and Klotho, a protein involved in anti-aging. METHODS: Healthy, sedentary men (n = 22) were randomized to moderate intensity training (MIT) or sprint intensity training (SIT) treatment groups. SIT consisted of 6 sessions over 2 weeks of 6 x 30 s all out cycle ergometer sprints with 4 min of recovery between sprints. MIT consisted of 6 sessions over 2 weeks of cycle ergometer exercise at 60% VO(2peak), gradually increasing in duration from 40 to 60 min. Blood was taken before the intervention and 48 h after the last training session, and glucose uptake was measured using [(18)F]FDG-PET/CT scanning. Cytokines were measured by multiplex and Klotho concentrations by ELISA. RESULTS: Both training protocols similarly increased VO(2peak) and decreased fat percentage and visceral fat (P < 0.05). MIT and SIT training programs both reduced the concentrations of IL-6, Hepatocyte Growth Factor (HGF) and Leptin. Interestingly, MIT, but not SIT increased monocyte chemoattractant protein-1 (MCP-1) concentrations, an exercise-induced cytokine, as well as Klotho concentrations. CONCLUSION: Short-term exercise training at markedly different intensities similarly improves cardiovascular fitness but results in intensity-specific changes in cytokine responses to exercise. FAU - Middelbeek, Roeland J W AU - Middelbeek RJW AUID- ORCID: 0000-0003-3862-8082 AD - Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Boston, MA, USA. AD - Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, 02215, USA. FAU - Motiani, Piryanka AU - Motiani P AD - Turku PET Centre, University of Turku, 20521, Turku, Finland. FAU - Brandt, Nina AU - Brandt N AUID- ORCID: 0000-0001-6833-0047 AD - Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Boston, MA, USA. FAU - Nigro, Pasquale AU - Nigro P AD - Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Boston, MA, USA. FAU - Zheng, Jia AU - Zheng J AD - Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Boston, MA, USA. FAU - Virtanen, Kirsi A AU - Virtanen KA AD - Turku PET Centre, Turku University Hospital, 20521, Turku, Finland. FAU - Kalliokoski, Kari K AU - Kalliokoski KK AD - Turku PET Centre, University of Turku, 20521, Turku, Finland. FAU - Hannukainen, Jarna C AU - Hannukainen JC AD - Turku PET Centre, University of Turku, 20521, Turku, Finland. FAU - Goodyear, Laurie J AU - Goodyear LJ AUID- ORCID: 0000-0002-6299-2928 AD - Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Boston, MA, USA. laurie.goodyear@joslin.harvard.edu. AD - Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA. laurie.goodyear@joslin.harvard.edu. LA - eng GR - 5P30DK36836/U.S. Department of Health & Human Services | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (National Institute of Diabetes & Digestive & Kidney Diseases)/International GR - R01 DK099511/DK/NIDDK NIH HHS/United States GR - R01 DK112283/DK/NIDDK NIH HHS/United States GR - P30 DK036836/DK/NIDDK NIH HHS/United States GR - R01-DK112283/U.S. Department of Health & Human Services | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (National Institute of Diabetes & Digestive & Kidney Diseases)/International GR - K23 DK114550/DK/NIDDK NIH HHS/United States PT - Journal Article PT - Randomized Controlled Trial PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20210107 PL - England TA - Nutr Diabetes JT - Nutrition & diabetes JID - 101566341 RN - 0 (CCL2 protein, human) RN - 0 (Chemokine CCL2) RN - 0 (Cytokines) RN - 0 (Interleukin-6) RN - 0 (Leptin) RN - 67256-21-7 (Hepatocyte Growth Factor) RN - EC 3.2.1.31 (Glucuronidase) RN - EC 3.2.1.31 (Klotho Proteins) RN - IY9XDZ35W2 (Glucose) SB - IM MH - Adult MH - Body Composition MH - Cardiorespiratory Fitness MH - Chemokine CCL2/blood MH - Cytokines/*blood MH - Endurance Training/methods MH - *Exercise MH - Glucose/metabolism MH - Glucuronidase/*blood MH - Healthy Lifestyle MH - Hepatocyte Growth Factor/blood MH - High-Intensity Interval Training/methods MH - Humans MH - Interleukin-6/blood MH - Klotho Proteins MH - Leptin/blood MH - Male MH - Middle Aged MH - Positron Emission Tomography Computed Tomography/methods PMC - PMC7791135 COIS- The authors declare that they have no conflict of interest. EDAT- 2021/01/09 06:00 MHDA- 2021/09/24 06:00 PMCR- 2021/01/07 CRDT- 2021/01/08 06:04 PHST- 2020/07/07 00:00 [received] PHST- 2020/11/16 00:00 [accepted] PHST- 2020/11/03 00:00 [revised] PHST- 2021/01/08 06:04 [entrez] PHST- 2021/01/09 06:00 [pubmed] PHST- 2021/09/24 06:00 [medline] PHST- 2021/01/07 00:00 [pmc-release] AID - 10.1038/s41387-020-00144-x [pii] AID - 144 [pii] AID - 10.1038/s41387-020-00144-x [doi] PST - epublish SO - Nutr Diabetes. 2021 Jan 7;11(1):5. doi: 10.1038/s41387-020-00144-x.