PMID- 33414645
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220419
IS  - 1179-1322 (Print)
IS  - 1179-1322 (Electronic)
IS  - 1179-1322 (Linking)
VI  - 12
DP  - 2020
TI  - Prognostic Value of Autophagy-related Proteins in Human Gastric Cancer.
PG  - 13527-13540
LID - 10.2147/CMAR.S278354 [doi]
AB  - PURPOSE: Autophagy-related proteins (ATG) play a crucial role in autophagy. 
      Recently, the functions of autophagy in cancer have been gathering attention. 
      However, the prognostic value of ATGs in gastric cancer (GC) has not been 
      explored. METHODS: The Kaplan-Meier plotter (KM plotter) online database was used 
      to examine the value of ATGs gene expression levels in overall survival (OS) 
      prediction in GC patients with different clinical stage, differentiation, gender, 
      HER2 status, and therapeutic strategy. In vitro experiments applied VE-822, an 
      effective GC treatment, to assess cell migration and proliferation in gastric 
      mucosa epithelial cells, and real-time PCR was used to measure alterations of 
      autophagy-related gene expression. RESULTS: High ATG3, ATG4C, ATG5, and ATG10 
      mRNA levels were associated with good OS, while increased ATG4B, ATG7, ATG12, 
      ATG16L1, and TECPR1 mRNA levels related to unfavorable OS in patients with GC. 
      ATG12 overexpression had different effects on OS due to high levels of 
      heterogeneity. High ATG12 expression was correlated with good OS in female 
      patients with GC and with bad OS for male patients. Additionally, the increased 
      ATG12 expression was more likely to get a satisfactory OS in patients who 
      underwent surgery alone but was associated with poor OS for patients treated with 
      5-FU adjuvant. In addition, elevated TECPR1 expression was related to favorable 
      OS for patients with poorly differentiated type, while for patients with moderate 
      differentiation, it was relevant to poor OS. The in vitro experiments showed that 
      berzosertib could significantly inhibit the migration and proliferation of human 
      gastric mucosa epithelial cells, and further real-time PCR assessment of ATG 
      expressions partially coincided with the bioinformation analysis above. 
      CONCLUSION: These results indicate that individual ATGs have unique prognostic 
      significance interpreted using Kaplan-Meier plotter analysis and in vitro 
      experiments, and this may help guide clinical therapeutic strategy and promote OS 
      by individualizing therapy for GC patients.
CI  - (c) 2020 Wu et al.
FAU - Wu, Minmin
AU  - Wu M
AUID- ORCID: 0000-0002-1704-7548
AD  - Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of 
      Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, 
      Wenzhou, Zhejiang Province 325000, People's Republic of China.
FAU - Chen, Bicheng
AU  - Chen B
AD  - Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of 
      Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, 
      Wenzhou, Zhejiang Province 325000, People's Republic of China.
FAU - Pan, Xiaodong
AU  - Pan X
AD  - Department of Transplantation Laboratory, The First Affiliated Hospital of 
      Wenzhou Medical University, Wenzhou, Zhejiang Province 325000, People's Republic 
      of China.
FAU - Su, Jiadong
AU  - Su J
AD  - Department of Traumatology, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang Province 325000, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20201231
PL  - New Zealand
TA  - Cancer Manag Res
JT  - Cancer management and research
JID - 101512700
PMC - PMC7783202
OTO - NOTNLM
OT  - autophagy-related proteins
OT  - gastric cancer
OT  - overall survival
OT  - prognostic values
COIS- The authors report no conflicts of interest in this work.
EDAT- 2021/01/09 06:00
MHDA- 2021/01/09 06:01
PMCR- 2020/12/31
CRDT- 2021/01/08 06:10
PHST- 2020/08/28 00:00 [received]
PHST- 2020/11/19 00:00 [accepted]
PHST- 2021/01/08 06:10 [entrez]
PHST- 2021/01/09 06:00 [pubmed]
PHST- 2021/01/09 06:01 [medline]
PHST- 2020/12/31 00:00 [pmc-release]
AID - 278354 [pii]
AID - 10.2147/CMAR.S278354 [doi]
PST - epublish
SO  - Cancer Manag Res. 2020 Dec 31;12:13527-13540. doi: 10.2147/CMAR.S278354. 
      eCollection 2020.