PMID- 33415855
OWN - NLM
STAT- MEDLINE
DCOM- 20210111
LR  - 20210111
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 45
IP  - 12
DP  - 2020 Dec 25
TI  - [Effects of electroacupuncture on proangiogenesis process and protein turnover in 
      a mouse model of sarcopenia].
PG  - 973-9
LID - 10.13702/j.1000-0607.200081 [doi]
AB  - OBJECTIVE: To observe the effect of electroacupuncture(EA) on proangiogenesis 
      process and protein turn-over in a mouse model of sarcopenia, so as to explore 
      its potential molecular mechanism anti-aging. METHODS: Fourteen 30-week-old male 
      SAMP8 mice were randomly divided into a model group (n=7) and an EA group (n=7). 
      Seven anti-rapidly aging SAMR1 mice of the same age were used as the control 
      group (n=7). EA (1 mA, 4 Hz) was applied to bilateral "Zusanli"(ST36) and 
      "Yanglingquan"(GB34) for 20 minutes each time once a day, 6 times a week for 4 
      weeks. The exhausted running platform was used to test the sports function. 
      Gastrocnemius muscle mass and relative ratio of gastrocnemius muscle mass to body 
      mass were measured. HE staining and transmission electron microscope were used to 
      observe the morphology, and the cross-sectional area of gastrocnemius muscle was 
      calculated. Relative protein expressions of protein kinase B (AKT) , 
      phosphorylated (p) -AKT, mammalian target of rapamycin (mTOR) , p-mTOR, p70 
      ribosomal protein S6 kinase (p70S6K) , p-p70S6K,hypoxia inducible factor-1alpha 
      (HIF-1alpha) and relative mRNA expressions of HIF-1alpha, vascular endothelial growth 
      factor A (VEGF-A) , muscle RING finger-1 (MuRF-1) and muscle atrophy F-box 
      (MAFbx) were detected by Western blot and real-time fluorescence quantitative 
      PCR, seperatively. RESULTS: Compared with the control group, the running time and 
      distance, body mass and gastrocnemius mass, and the ratio of gastrocnemius mass 
      to body mass decreased(P<0.01, P<0.05), cross-sectional area of gastrocnemius, 
      related protein expression of p-AKT,p-mTOR, p-p70S6K and HIF-1alpha, mRNA expression 
      of HIF-1alpha and VEGF-A decreased (P<0.01), while mRNA expression of MuRF1 and MAFbx 
      increased (P<0.01) in the model group. Following EA intervention, the running 
      time and distance, body mass and gastrocnemius mass and the ratio of 
      gastrocnemius mass to body mass increased (P<0.05), cross-sectional area of 
      gastrocnemius, related protein expression of p-AKT,p-mTOR, p-p70S6K and HIF-1alpha, 
      mRNA expression of HIF-1alpha and VEGF-A were significantly up-regulated (P<0.01), 
      mRNA expression of MuRF1 and MAFbx down-regulated (P<0.01, P<0.05) in the EA 
      group compared with the model group. CONCLUSION: EA may delay the aging muscle 
      atrophy in mice by regulating the gastrocnemius muscle's proangiogenesis process 
      and protein turnover.
FAU - Zhu, Zheng-Wei
AU  - Zhu ZW
AD  - College of Traditional Chinese Medicine of Chongqing Medical University, 
      Chongqing 400016, China.
FAU - Tang, Cheng-Lin
AU  - Tang CL
AD  - College of Traditional Chinese Medicine of Chongqing Medical University, 
      Chongqing 400016, China.
FAU - Li, Xiao-Hong
AU  - Li XH
AD  - Department of Rehabilitation, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016.
FAU - Yang, Zhi-Xue
AU  - Yang ZX
AD  - College of Traditional Chinese Medicine of Chongqing Medical University, 
      Chongqing 400016, China.
FAU - Yang, Yun-Hao
AU  - Yang YH
AD  - College of Traditional Chinese Medicine of Chongqing Medical University, 
      Chongqing 400016, China.
FAU - Pang, Fang
AU  - Pang F
AD  - College of Traditional Chinese Medicine of Chongqing Medical University, 
      Chongqing 400016, China.
FAU - Liao, Dong-Mei
AU  - Liao DM
AD  - College of Traditional Chinese Medicine of Chongqing Medical University, 
      Chongqing 400016, China.
FAU - Guo, Xiao
AU  - Guo X
AD  - College of Traditional Chinese Medicine of Chongqing Medical University, 
      Chongqing 400016, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - *Electroacupuncture
MH  - Male
MH  - Mice
MH  - Muscular Atrophy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Sarcopenia
MH  - Vascular Endothelial Growth Factor A/genetics
OTO - NOTNLM
OT  - Angiogenesisa
OT  - Electroacupuncturea
OT  - Hypoxiaa
OT  - Protein turnover
OT  - Sarcopeniaa
EDAT- 2021/01/09 06:00
MHDA- 2021/01/12 06:00
CRDT- 2021/01/08 06:21
PHST- 2021/01/08 06:21 [entrez]
PHST- 2021/01/09 06:00 [pubmed]
PHST- 2021/01/12 06:00 [medline]
AID - 10.13702/j.1000-0607.200081 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2020 Dec 25;45(12):973-9. doi: 10.13702/j.1000-0607.200081.