PMID- 33416114
OWN - NLM
STAT- MEDLINE
DCOM- 20210716
LR  - 20210716
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 47
IP  - 1
DP  - 2021 Jan
TI  - Prostaglandin E2 serves a dual role in regulating the migration of dendritic 
      cells.
PG  - 207-218
LID - 10.3892/ijmm.2020.4801 [doi]
AB  - Dendritic cells (DCs) are the most potent antigen‑presenting cells, and are 
      indispensable in the immune system. Prostaglandin E2 (PGE2) has been demonstrated 
      to modulate the migration of DCs, but with inconsistent results. The present 
      study, based on our previous research, used murine bone marrow‑derived DCs to 
      elucidate the potential regulatory mechanism of PGE2 on the migration of DCs. The 
      results indicated that PGE2 served a dual role in regulating the migration of DCs 
      in a dose‑dependent manner. High concentrations of PGE2 inhibited cell migration, 
      whereas low concentrations exhibited the opposite effect. Flow cytometry revealed 
      that the expression of CC chemokine receptor type 7 on the DC surface was 
      increased following treatment with low concentrations of PGE2 and slightly 
      decreased by high concentrations of PGE2. The effect of PGE2 was indicated to be 
      exerted via reorganizing the F‑actin cytoskeleton using confocal microscopy. 
      Moreover, the regulatory effect of PGE2 on the migration of DCs was validated 
      in vivo. Subsequent gene expression profile analyses using RNA‑sequencing 
      technology indicated that PGE2 induced alterations in the expression of multiple 
      downstream genes and signaling pathway molecules associated with cell migration 
      and the cytoskeleton. These findings may provide an improved understanding on the 
      mechanism of DC migration under both pathological and physiological conditions. 
      Moreover, the biological implications of these findings may provide a novel 
      perspective of the immunological surveillance in the progression of different 
      types of diseases.
FAU - Diao, Ge
AU  - Diao G
AD  - Department of Gynecology and Obstetrics, Daping Hospital, Army Medical University 
      (Third Military Medical University), Chongqing 400042, P.R. China.
FAU - Huang, Jie
AU  - Huang J
AD  - Department of Gynecology and Obstetrics, Daping Hospital, Army Medical University 
      (Third Military Medical University), Chongqing 400042, P.R. China.
FAU - Zheng, Xiuhui
AU  - Zheng X
AD  - Department of Gynecology and Obstetrics, Daping Hospital, Army Medical University 
      (Third Military Medical University), Chongqing 400042, P.R. China.
FAU - Sun, Xinwei
AU  - Sun X
AD  - Department of Gynecology and Obstetrics, Daping Hospital, Army Medical University 
      (Third Military Medical University), Chongqing 400042, P.R. China.
FAU - Tian, Min
AU  - Tian M
AD  - Department of Gynecology and Obstetrics, Daping Hospital, Army Medical University 
      (Third Military Medical University), Chongqing 400042, P.R. China.
FAU - Han, Jian
AU  - Han J
AD  - Department of Gynecology and Obstetrics, Daping Hospital, Army Medical University 
      (Third Military Medical University), Chongqing 400042, P.R. China.
FAU - Guo, Jianxin
AU  - Guo J
AD  - Department of Gynecology and Obstetrics, Daping Hospital, Army Medical University 
      (Third Military Medical University), Chongqing 400042, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20201126
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Cell Movement/*immunology
MH  - Dendritic Cells/cytology/*immunology
MH  - Dinoprostone/*immunology
MH  - Male
MH  - Mice
PMC - PMC7723496
OTO - NOTNLM
OT  - prostaglandin E2
OT  - dendritic cells
OT  - cell migration
OT  - dual role
OT  - RNA-sequencing
EDAT- 2021/01/09 06:00
MHDA- 2021/07/17 06:00
PMCR- 2020/11/26
CRDT- 2021/01/08 08:37
PHST- 2020/07/08 00:00 [received]
PHST- 2020/11/04 00:00 [accepted]
PHST- 2021/01/09 06:00 [pubmed]
PHST- 2021/07/17 06:00 [medline]
PHST- 2021/01/08 08:37 [entrez]
PHST- 2020/11/26 00:00 [pmc-release]
AID - ijmm-47-01-0207 [pii]
AID - 10.3892/ijmm.2020.4801 [doi]
PST - ppublish
SO  - Int J Mol Med. 2021 Jan;47(1):207-218. doi: 10.3892/ijmm.2020.4801. Epub 2020 Nov 
      26.