PMID- 33421161
OWN - NLM
STAT- MEDLINE
DCOM- 20210423
LR  - 20210423
IS  - 1399-3054 (Electronic)
IS  - 0031-9317 (Linking)
VI  - 172
IP  - 1
DP  - 2021 May
TI  - Implication of actin in the uptake of sucrose and valine in the tap root and leaf 
      of sugar beet.
PG  - 218-232
LID - 10.1111/ppl.13322 [doi]
AB  - Actin microfilaments (F-actin) are major components of the cytoskeleton essential 
      for many cellular dynamic processes (vesicle trafficking, cytoplasmic streaming, 
      organelle movements). The aim of this study was to examine whether cortical actin 
      microfilaments might be implicated in the regulation of nutrient uptake in root 
      and leaf cells of Beta vulgaris. Using antibodies raised against actin and the 
      AtSUC1 sucrose transporter, immunochemical assays demonstrated that the 
      expression of actin and a sucrose transporter showed different characteristics, 
      when detected on plasma membrane vesicles (PMVs) purified from roots and from 
      leaves. The in situ immunolabeling of actin and AtSUC1 sites in PMVs and tissues 
      showed their close proximity to the plasma membrane. Using co-labeling in 
      protoplasts, actin and sucrose transporters were localized along the internal 
      border and in the outermost part of the plasma membrane, respectively. This 
      respective membrane co-localization was confirmed on PMVs and in tissues using 
      transmission electronic microscopy. The possible functional role of actin in 
      sucrose uptake (and valine uptake, comparatively) by PMVs and tissues from roots 
      and leaves was examined using the pharmacological inhibitors, cytochalasin B 
      (CB), cytochalasin D (CD), and phalloidin (PH). CB and CD inhibited the sucrose 
      and valine uptake by root tissues in a concentration-dependent manner above 1 muM, 
      whereas PH had no such effect. Comparatively, the toxins inhibited the sucrose 
      and valine uptake in leaf discs to a lesser extent. The inhibition was not due to 
      a hindering of the proton pumping and H(+) -ATPase catalytic activity determined 
      in PMVs incubated in presence of these toxins.
CI  - (c) 2021 Scandinavian Plant Physiology Society.
FAU - Michonneau, Philippe
AU  - Michonneau P
AD  - Pole Agronomie Innovation Services, SCARA, Villette-sur-Aube, France.
AD  - Laboratoire EBI (Ecologie et Biologie des Interactions, Universite de Poitiers, 
      Poitiers, France.
FAU - Fleurat-Lessard, Pierrette
AU  - Fleurat-Lessard P
AD  - Laboratoire EBI (Ecologie et Biologie des Interactions, Universite de Poitiers, 
      Poitiers, France.
FAU - Cantereau, Anne
AU  - Cantereau A
AD  - Plateforme ImageUP, Signalisation & Transports Ioniques Membranaires CNRS 6187, 
      Poitiers, France.
FAU - Crepin, Alexandre
AU  - Crepin A
AD  - Laboratoire EBI (Ecologie et Biologie des Interactions, Universite de Poitiers, 
      Poitiers, France.
FAU - Roblin, Gabriel
AU  - Roblin G
AD  - Laboratoire EBI (Ecologie et Biologie des Interactions, Universite de Poitiers, 
      Poitiers, France.
FAU - Berjeaud, Jean-Marc
AU  - Berjeaud JM
AD  - Laboratoire EBI (Ecologie et Biologie des Interactions, Universite de Poitiers, 
      Poitiers, France.
LA  - eng
GR  - 2015-2020 programs/State-Region Planning Contracts (CPER) and the European 
      Regional Development Fund (FEDER)/
PT  - Journal Article
DEP - 20210122
PL  - Denmark
TA  - Physiol Plant
JT  - Physiologia plantarum
JID - 1256322
RN  - 0 (Actins)
RN  - 57-50-1 (Sucrose)
RN  - HG18B9YRS7 (Valine)
SB  - IM
MH  - Actins
MH  - *Beta vulgaris
MH  - Plant Leaves
MH  - Sucrose
MH  - Valine
EDAT- 2021/01/10 06:00
MHDA- 2021/04/24 06:00
CRDT- 2021/01/09 17:09
PHST- 2020/12/15 00:00 [received]
PHST- 2020/12/18 00:00 [accepted]
PHST- 2021/01/10 06:00 [pubmed]
PHST- 2021/04/24 06:00 [medline]
PHST- 2021/01/09 17:09 [entrez]
AID - 10.1111/ppl.13322 [doi]
PST - ppublish
SO  - Physiol Plant. 2021 May;172(1):218-232. doi: 10.1111/ppl.13322. Epub 2021 Jan 22.