PMID- 33421202
OWN - NLM
STAT- MEDLINE
DCOM- 20210823
LR  - 20220531
IS  - 1399-3038 (Electronic)
IS  - 0905-6157 (Print)
IS  - 0905-6157 (Linking)
VI  - 32
IP  - 4
DP  - 2021 May
TI  - Association between human leukocyte antigens (HLAs) and human neutrophil antigens 
      (HNAs) and autoimmune neutropenia of infancy in Danish patients.
PG  - 756-761
LID - 10.1111/pai.13450 [doi]
AB  - BACKGROUND: Autoimmune neutropenia of infancy (AIN) is a frequent cause of 
      neutropenia in children. The disease is caused by antibodies against epitopes on 
      the immunoglobulin G (IgG) Fc receptor type 3b (FcgammaIIIb). We investigated the 
      possible association of human neutrophil antigens (HNA), human leukocyte antigen 
      (HLA)-DR, and HLA-DQ alleles with AIN and the association of these genotypes with 
      the presence of autoantibodies. METHODS: Eighty AIN cases with a median age of 
      13.5 months were included. Controls were healthy unrelated Danish blood donors. 
      Anti-HNA-1a autoantibodies were detected using a flow cytometric granulocyte 
      immunofluorescence test (Flow-GIFT) with phenotyped donor cells for detection of 
      antibody specificity. Molecular determination of HNA genotypes was determined 
      using real-time polymerase chain reaction (q-PCR). High-resolution HLA-DRB1 and 
      HLA-DQB1 were determined by next-generation sequencing. RESULTS: Antibodies 
      against HNA-1a were detected in 51% (n = 41) of AIN patients, and anti-HNA-1b was 
      detected in 3% (n = 2) of cases. In 46% of cases, the antibodies were 
      anti-FcgammaIIIb-reactive. FCGR3B*01+,*02-,*03- was more common (odds ratio, 6.70; 
      P < .0001), and FCGR3B*01-,*02+,*03- was less common (odds ratio, 0.30; 
      P < .0001) among AIN cases. HNA-1a antibodies were significantly more frequent 
      among AIN cases with the FCGR3B*01+,*02-,*03- genotype (odds ratio, 3.86; 
      P < .007). The HLA-DRB1*14 - HLA-DQB1*05:03 haplotype was significantly more 
      common (odds ratio, 7.44; P < .0001) in AIN patients. CONCLUSION: The HLA 
      haplotype HLA-DRB1*14 - DQB1*05:03 is associated with Danish AIN cases. Among 
      Danish AIN patients, anti-HNA-1a is the most common autoantibody, and the 
      antibody is more common in cases with the FCGR3B*01+,*02-,*03- genotype.
CI  - (c) 2021 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.
FAU - Nielsen, Kaspar Rene
AU  - Nielsen KR
AUID- ORCID: 0000-0003-4406-9643
AD  - Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark.
FAU - Bojsen, Signe Rolskov
AU  - Bojsen SR
AD  - Department of Clinical Genetics, Sygehus Lillebaelt Vejle Hospital, Vejle, 
      Denmark.
FAU - Masmas, Tania Nicole
AU  - Masmas TN
AD  - Pediatric Hematopoietic Stem Cell Transplantation and Immunodeficiency, The Child 
      and Adolescent Clinic, Rigshospitalet, Copenhagen University Hospital, 
      Copenhagen, Denmark.
FAU - Fjordside, Anne-Louise
AU  - Fjordside AL
AD  - Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark.
FAU - Baech, John
AU  - Baech J
AD  - Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark.
FAU - Haunstrup, Thure Mors
AU  - Haunstrup TM
AD  - Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark.
FAU - Steffensen, Rudi
AU  - Steffensen R
AD  - Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark.
LA  - eng
PT  - Journal Article
DEP - 20210209
PL  - England
TA  - Pediatr Allergy Immunol
JT  - Pediatric allergy and immunology : official publication of the European Society 
      of Pediatric Allergy and Immunology
JID - 9106718
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Autoimmunity
MH  - Denmark
MH  - Genotype
MH  - HLA Antigens
MH  - Humans
MH  - Infant
MH  - *Neutropenia
MH  - *Neutrophils
PMC - PMC8248029
OTO - NOTNLM
OT  - HLA
OT  - HNA
OT  - autoimmune
OT  - genotype
OT  - neutropenia
COIS- On behalf of all authors, I hereby declare no conflicts of interest.
EDAT- 2021/01/10 06:00
MHDA- 2021/08/24 06:00
PMCR- 2021/07/01
CRDT- 2021/01/09 17:10
PHST- 2020/12/09 00:00 [revised]
PHST- 2020/08/04 00:00 [received]
PHST- 2020/12/16 00:00 [accepted]
PHST- 2021/01/10 06:00 [pubmed]
PHST- 2021/08/24 06:00 [medline]
PHST- 2021/01/09 17:10 [entrez]
PHST- 2021/07/01 00:00 [pmc-release]
AID - PAI13450 [pii]
AID - 10.1111/pai.13450 [doi]
PST - ppublish
SO  - Pediatr Allergy Immunol. 2021 May;32(4):756-761. doi: 10.1111/pai.13450. Epub 
      2021 Feb 9.