PMID- 33422081
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210114
IS  - 1475-2867 (Print)
IS  - 1475-2867 (Electronic)
IS  - 1475-2867 (Linking)
VI  - 21
IP  - 1
DP  - 2021 Jan 9
TI  - LncRNA CBR3-AS1 potentiates Wnt/beta-catenin signaling to regulate lung 
      adenocarcinoma cells proliferation, migration and invasion.
PG  - 36
LID - 10.1186/s12935-020-01685-y [doi]
LID - 36
AB  - BACKGROUND: Long non-coding RNAs (lncRNAs) are pervasively transcribed in genome 
      and emerging as a new player in tumorigenesis due to their functions in 
      transcriptional, posttranscriptional and epigenetic mechanisms of gene 
      regulation. As the most frequent malignancy and the foremost source of cancer 
      mortality, lung cancer is a heterogeneous disorder. The most common type of lung 
      cancer is Non-small cell lung cancer (NSCLC), occupying 85% of the total cases, 
      and the main subtypes of NSCLC include lung adenocarcinoma (LAD), large cell 
      carcinoma (LCC), and lung squamous cell carcinoma (LSCC). Recently, numerous 
      lncRNAs have been reported to be strongly linked to NSCLC. In the present study, 
      we found that a new lncRNA CBR3-AS1 is highly expressed in lung cancer. In 
      addition, we also examined the expression of lncRNA CBR3-AS1 in 60 of LADs, 40 of 
      LCCs and 40 of LSCCs patient samples, finding that CBR3-AS1 was specificity 
      highly expressed in LAD cancer tissues. Mechanically, we discovered that CBR3-AS1 
      could regulate the proliferation, migration and invasion of LAD cells through 
      targeting Wnt/beta-catenin signaling. METHODS: Real-time PCR, RNA-pulldown, RIP, 
      western blotting, lentivirus transfection, luciferase reporter assays, cell 
      proliferation assays, colony formation assays, wound healing scratch assays and 
      transwell assays were employed to examine the relationship between lncRNA 
      CBR3-AS1 and its regulation of Wnt/beta-catenin signaling in LAD cells. RESULTS: 
      LncRNA CBR3-AS1 is highly-expressed in LAD and cell lines. LncRNA CBR3-AS1 shows 
      physical association with beta-catenin. CBR3-AS1 could facilitate Wnt/beta-catenin 
      signaling activation thought promoting nuclear localization of beta-catenin. 
      CBR3-AS1 promotes LAD cell proliferation, migration and invasion by targeting 
      Wnt/beta-catenin signaling. CONCLUSION: It can be found that a new functional lncRNA 
      CBR3-AS1 could promote nuclear localization of beta-catenin so as to facilitate 
      Wnt/beta-catenin signaling activation and regulate the proliferation, migration and 
      invasion of LAD cells.
FAU - Hou, Min
AU  - Hou M
AUID- ORCID: 0000-0003-2076-1387
AD  - Clinical Laboratory, Tianjin Chest Hospital, No. 261, South Taierzhuang Road, 
      Tianjin, 300222, China. hmxkyy@163.com.
FAU - Wu, Nannan
AU  - Wu N
AD  - Clinical Laboratory, Tianjin Chest Hospital, No. 261, South Taierzhuang Road, 
      Tianjin, 300222, China.
FAU - Yao, Lili
AU  - Yao L
AD  - Clinical Laboratory, Tianjin Chest Hospital, No. 261, South Taierzhuang Road, 
      Tianjin, 300222, China.
LA  - eng
GR  - [Jin] 20160704/Tianjin Scientific and Technological Foundation/
GR  - [Jin] 20171008/Tianjin Scientific and Technological Foundation/
PT  - Journal Article
DEP - 20210109
PL  - England
TA  - Cancer Cell Int
JT  - Cancer cell international
JID - 101139795
PMC - PMC7796595
COIS- The authors declare that they have no competing interests.
EDAT- 2021/01/11 06:00
MHDA- 2021/01/11 06:01
PMCR- 2021/01/09
CRDT- 2021/01/10 20:24
PHST- 2019/11/18 00:00 [received]
PHST- 2020/11/27 00:00 [accepted]
PHST- 2021/01/10 20:24 [entrez]
PHST- 2021/01/11 06:00 [pubmed]
PHST- 2021/01/11 06:01 [medline]
PHST- 2021/01/09 00:00 [pmc-release]
AID - 10.1186/s12935-020-01685-y [pii]
AID - 1685 [pii]
AID - 10.1186/s12935-020-01685-y [doi]
PST - epublish
SO  - Cancer Cell Int. 2021 Jan 9;21(1):36. doi: 10.1186/s12935-020-01685-y.