PMID- 33422798
OWN - NLM
STAT- MEDLINE
DCOM- 20210127
LR  - 20210127
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1637
DP  - 2021 Jan 25
TI  - A porous layer open-tubular capillary column supported with pepsin and zeolitic 
      imidazolate framework for enantioseparation of four basic drugs in capillary 
      electrochromatography.
PG  - 461866
LID - S0021-9673(20)31140-7 [pii]
LID - 10.1016/j.chroma.2020.461866 [doi]
AB  - New material zeolitic imidazolate framework-4, 5-imidazoledicarboxylic acid 
      (ZIF-IMD) located on the pore surface of porous layer open-tubular (PLOT) column 
      previously functionalized with N-(3-aminopropyl)-imidazole have been prepared via 
      a layer-by-layer self-assembly strategy. This new ZIF-IMD coating hybrids are 
      used as solid-phase carriers for chiral selector pepsin immobilization. The 
      ZIF-IMD material was characterized by scanning electron microscopy, 
      energy-dispersive spectroscopy, transmission electron microscope and X-ray 
      diffraction. The synthesized pepsin@ZIF-IMD@POLT column achieved the baseline 
      separation of hydroxychloroquine (HCQ), chloroquine (CHQ) and hydroxyzine (HXY) 
      (the resolution of HCQ: 2.19; CHQ: 1.84; HXY: 1.53). Compared with the 
      pepsin@PLOT column (without ZIF-IMD material), the chiral separation capability 
      of the pepsin@ZIF-IMD@POLT column can be remarkably improved. Several key 
      parameters including concentration of chiral selector, buffer pH, applied voltage 
      and buffer concentration were systematically evaluated to provide the optimal 
      enantioseparation condition. The relative standard deviations (RSDs) of 
      intra-day, inter-day, column-to-column and inter-batch of migration time and Rs 
      of the HCQ were evaluated in detail, respectively (RSD < 7.21%). Additionally, 
      the potential mechanism of increased resolution was discussed in the article.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Ma, Mingxuan
AU  - Ma M
AD  - Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of 
      Education), China Pharmaceutical University, Nanjing 210009, PR China; State Key 
      Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, 
      PR China.
FAU - Chen, Cheng
AU  - Chen C
AD  - Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of 
      Education), China Pharmaceutical University, Nanjing 210009, PR China; State Key 
      Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, 
      PR China.
FAU - Zhu, Xinqi
AU  - Zhu X
AD  - Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of 
      Education), China Pharmaceutical University, Nanjing 210009, PR China; State Key 
      Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, 
      PR China.
FAU - Li, Xiaoqi
AU  - Li X
AD  - Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, 
      Nanjing University of Chinese Medicine, Nanjing 210028, PR China. Electronic 
      address: xiaoqili8619@163.com.
FAU - Du, Yingxiang
AU  - Du Y
AD  - Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of 
      Education), China Pharmaceutical University, Nanjing 210009, PR China; State Key 
      Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, 
      PR China. Electronic address: du_yingxiang@126.com.
FAU - Zhang, Liu
AU  - Zhang L
AD  - Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of 
      Education), China Pharmaceutical University, Nanjing 210009, PR China; State Key 
      Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, 
      PR China.
FAU - Gan, Jie
AU  - Gan J
AD  - Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of 
      Education), China Pharmaceutical University, Nanjing 210009, PR China; State Key 
      Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, 
      PR China.
LA  - eng
PT  - Journal Article
DEP - 20201231
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Buffers)
RN  - 0 (Imidazoles)
RN  - 0 (Pharmaceutical Preparations)
RN  - 1318-02-1 (Zeolites)
RN  - 7GBN705NH1 (imidazole)
RN  - EC 3.4.23.1 (Pepsin A)
SB  - IM
MH  - Buffers
MH  - Capillary Electrochromatography/*methods
MH  - Hydrogen-Ion Concentration
MH  - Imidazoles/*chemistry
MH  - Microscopy, Electron, Scanning
MH  - Pepsin A/*chemistry
MH  - Pharmaceutical Preparations/*chemistry/*isolation & purification
MH  - Porosity
MH  - Reproducibility of Results
MH  - Stereoisomerism
MH  - Zeolites/*chemistry
OTO - NOTNLM
OT  - 4,5-imidazoledicarboxylic acid
OT  - Capillary electrochromatography
OT  - Chiral separation
OT  - Organic hybrid coating
OT  - PLOT
OT  - Zeolitic imidazolate framework
COIS- Declaration of Competing Interest The authors have declared no conflict of 
      interest.
EDAT- 2021/01/11 06:00
MHDA- 2021/01/28 06:00
CRDT- 2021/01/10 20:34
PHST- 2020/11/05 00:00 [received]
PHST- 2020/12/23 00:00 [revised]
PHST- 2020/12/28 00:00 [accepted]
PHST- 2021/01/11 06:00 [pubmed]
PHST- 2021/01/28 06:00 [medline]
PHST- 2021/01/10 20:34 [entrez]
AID - S0021-9673(20)31140-7 [pii]
AID - 10.1016/j.chroma.2020.461866 [doi]
PST - ppublish
SO  - J Chromatogr A. 2021 Jan 25;1637:461866. doi: 10.1016/j.chroma.2020.461866. Epub 
      2020 Dec 31.