PMID- 33425299
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210112
IS  - 2008-9783 (Print)
IS  - 2228-6721 (Electronic)
IS  - 2008-9783 (Linking)
VI  - 11
IP  - 4
DP  - 2020 Fall
TI  - Photobiomodulation Therapy in the Proliferation and Differentiation of Human 
      Umbilical Cord Mesenchymal Stem Cells: An In Vitro Study.
PG  - 469-474
LID - 10.34172/jlms.2020.73 [doi]
AB  - Introduction: Since photobiomodulation therapy (PBMT) favors in vitro mesenchymal 
      stem cell (MSC) preconditioning before MSC transplantation, increasing the 
      proliferation of these cells without molecular injuries by conserving their 
      characteristics, in the present in vitro study we analyzed the effect of PBMT on 
      the proliferation and osteogenic differentiation of human umbilical cord 
      mesenchymal stem cells (hUCMSCs). Methods: Irradiation with an InGaAIP Laser (660 
      nm, 10 mW, 2.5 J/cm(2) , 0.08 cm(2) spot size, and 10 s) was carried out. The 
      cells were divided into four groups: CONTROL [cells grown in Dulbecco's Modified 
      Eagle Medium (DMEM)], OSTEO (cells grown in an osteogenic medium); PBMT (cells 
      grown in DMEM+PBMT), and OSTEO+PBMT (cells grown in an osteogenic medium plus 
      PBMT). The cell proliferation curve was obtained over periods of 24, 48 and 72 
      hours using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide 
      (MTT) assay. Osteogenic differentiation was analyzed by the formation of calcium 
      nodules over periods of 7, 14 and 21 days. Morphometric analysis was performed to 
      quantify the total area of nodular calcification. Results: The highest cell 
      proliferation and cell differentiation occurred in the OSTEO+PBMT group, followed 
      by the PBMT, OSTEO and CONTROL groups respectively, at the observed times (P 
      <0.05). Conclusion: PBMT enhanced the osteogenic proliferation and the 
      differentiation of hUCMSCs during the periods tested, without causing damage to 
      the cells and preserving their specific characteristics, a fact that may 
      represent an innovative pretreatment in the application of stem cells.
CI  - Copyright (c) 2020 J Lasers Med Sci.
FAU - Miranda, Jessica Meirinhos
AU  - Miranda JM
AD  - Laser Center, School of Dentistry, Universidade de Pernambuco, Camaragibe, PE, 
      Brazil.
AD  - Department of Restorative Dentistry and Endodontics, School of Dentistry, 
      Universidade de Pernambuco, Camaragibe, PE, Brazil.
FAU - de Arruda, Jose Alcides Almeida
AU  - de Arruda JAA
AD  - Department of Oral Surgery and Pathology, School of Dentistry, Universidade 
      Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Moreno, Lara Marques Magalhaes
AU  - Moreno LMM
AD  - Laser Center, School of Dentistry, Universidade de Pernambuco, Camaragibe, PE, 
      Brazil.
AD  - Department of Restorative Dentistry and Endodontics, School of Dentistry, 
      Universidade de Pernambuco, Camaragibe, PE, Brazil.
FAU - Gaiao, Wyndly Daniel Cardoso
AU  - Gaiao WDC
AD  - Department of Biophysics and Radiobiology, Universidade Federal de Pernambuco, 
      Recife, PE, Brazil.
FAU - do Nascimento, Sinval Vinicius Barbosa
AU  - do Nascimento SVB
AD  - Laser Center, School of Dentistry, Universidade de Pernambuco, Camaragibe, PE, 
      Brazil.
AD  - Department of Restorative Dentistry and Endodontics, School of Dentistry, 
      Universidade de Pernambuco, Camaragibe, PE, Brazil.
FAU - Silva, Eduardo Vinicius de Souza
AU  - Silva EVS
AD  - Laser Center, School of Dentistry, Universidade de Pernambuco, Camaragibe, PE, 
      Brazil.
AD  - Department of Restorative Dentistry and Endodontics, School of Dentistry, 
      Universidade de Pernambuco, Camaragibe, PE, Brazil.
FAU - da Silva, Marcia Bezerra
AU  - da Silva MB
AD  - Department of Biophysics and Radiobiology, Universidade Federal de Pernambuco, 
      Recife, PE, Brazil.
FAU - Rodrigues, Claudio Gabriel
AU  - Rodrigues CG
AD  - Department of Biophysics and Radiobiology, Universidade Federal de Pernambuco, 
      Recife, PE, Brazil.
FAU - de Albuquerque, Diana Santana
AU  - de Albuquerque DS
AD  - Department of Restorative Dentistry and Endodontics, School of Dentistry, 
      Universidade de Pernambuco, Camaragibe, PE, Brazil.
FAU - Braz, Rodivan
AU  - Braz R
AD  - Department of Restorative Dentistry and Endodontics, School of Dentistry, 
      Universidade de Pernambuco, Camaragibe, PE, Brazil.
FAU - Pinheiro, Antonio Luiz Barbosa
AU  - Pinheiro ALB
AD  - Center of Biophotonics, School of Dentistry, Universidade Federal da Bahia, 
      Salvador, BA, Brazil.
FAU - Gerbi, Marleny Elizabeth Marquez de Martinez
AU  - Gerbi MEMM
AD  - Laser Center, School of Dentistry, Universidade de Pernambuco, Camaragibe, PE, 
      Brazil.
AD  - Department of Restorative Dentistry and Endodontics, School of Dentistry, 
      Universidade de Pernambuco, Camaragibe, PE, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20201003
PL  - Iran
TA  - J Lasers Med Sci
JT  - Journal of lasers in medical sciences
JID - 101580415
PMC - PMC7736934
OTO - NOTNLM
OT  - Cell differentiation
OT  - Lasers
OT  - Photobiomodulation
OT  - Stem cells
EDAT- 2021/01/12 06:00
MHDA- 2021/01/12 06:01
PMCR- 2020/09/01
CRDT- 2021/01/11 05:39
PHST- 2021/01/11 05:39 [entrez]
PHST- 2021/01/12 06:00 [pubmed]
PHST- 2021/01/12 06:01 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - 10.34172/jlms.2020.73 [doi]
PST - ppublish
SO  - J Lasers Med Sci. 2020 Fall;11(4):469-474. doi: 10.34172/jlms.2020.73. Epub 2020 
      Oct 3.