PMID- 33425363 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20210112 IS - 2052-2975 (Print) IS - 2052-2975 (Electronic) IS - 2052-2975 (Linking) VI - 39 DP - 2021 Jan TI - Microscopic detection of bacillus Calmette-Guerin mycobacteria in bladder biopsy using fluorescence in situ hybridization: Detection microscopique des Bacilles bilies de Calmette et Guerin (BCG) dans une biopsie vesicale par hybridation in situ en fluorescence. PG - 100826 LID - 10.1016/j.nmni.2020.100826 [doi] LID - 100826 AB - Intravesical instillation of Bacilli Calmette Guerin (BCG) as a superficial bladder cancer treatment is generally well tolerated, but local or systemic complications may occur, some of which may be life-threatening. Following the suspicion of post-BCG cystitis in a 72-year-old man with a history of urothelial carcinoma treated by intravesical BCG instillation, we used fluorescence in situ hybridization (FISH) targeting the rpoB gene of the Mycobacterium tuberculosis complex to detect Mycobacterium bovis BCG in paraffin-embedded bladder biopsy sections. FISH yielded specific detection of BCG mycobacteria in the bladder biopsy section, appearing as red-fluorescent bacilli. Treatment with rifampicin, ethambutol and isoniazid is then initiated in combination with corticosteroid therapy. CI - (c) 2020 Published by Elsevier Ltd. FAU - Loukil, A AU - Loukil A AD - Aix-Marseille Universite, IRD, MEPHI, IHU Mediterranee-Infection, Marseille, France. FAU - Baron, S A AU - Baron SA AD - Aix-Marseille Universite, IRD, MEPHI, IHU Mediterranee-Infection, Marseille, France. FAU - Argemi, X AU - Argemi X AD - GIE Almaviva Sante, Clinique Axium, Aix en Provence, France. FAU - Maubon, T AU - Maubon T AD - Hopital Prive Residence Du Parc, Marseille, France. FAU - Eldin, C AU - Eldin C AD - Aix-Marseille Universite, IRD, MEPHI, IHU Mediterranee-Infection, Marseille, France. LA - eng PT - Case Reports DEP - 20201126 PL - England TA - New Microbes New Infect JT - New microbes and new infections JID - 101624750 PMC - PMC7785951 OTO - NOTNLM OT - Bladder cancer OT - Mycobacterium bovis BCG OT - Mycobacterium tuberculosis complex OT - fluorescence in situ hybridization OT - microscopic examination EDAT- 2021/01/12 06:00 MHDA- 2021/01/12 06:01 PMCR- 2020/11/26 CRDT- 2021/01/11 05:39 PHST- 2020/09/07 00:00 [received] PHST- 2020/11/12 00:00 [revised] PHST- 2020/11/22 00:00 [accepted] PHST- 2021/01/11 05:39 [entrez] PHST- 2021/01/12 06:00 [pubmed] PHST- 2021/01/12 06:01 [medline] PHST- 2020/11/26 00:00 [pmc-release] AID - S2052-2975(20)30178-5 [pii] AID - 100826 [pii] AID - 10.1016/j.nmni.2020.100826 [doi] PST - epublish SO - New Microbes New Infect. 2020 Nov 26;39:100826. doi: 10.1016/j.nmni.2020.100826. eCollection 2021 Jan.