PMID- 33426545
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220813
VI  - 3
IP  - Suppl 1
DP  - 2020
TI  - ERBB2 FISH and Chromosome Microarray Testing of Gastroesophageal Adenocarcinomas 
      at a Single Institution.
PG  - 39-46
AB  - Overexpression/amplification of erb-b2 receptor tyrosine kinase 2 (ERBB2) is a 
      major prognostic factor in gastroesophageal cancers; it is currently the only 
      biomarker established for the selection of targeted therapy for patients with 
      advanced gastroesophageal adenocarcinoma (GEA). Current standard procedure for 
      determining ERBB2 status in such patients is immunohistochemistry (IHC), followed 
      by in situ hybridization (ISH), when IHC result is equivocal. Insufficient 
      knowledge regarding the utilities of chromosomal microarray (CMA) has hindered 
      its use as an adjunct tool in ERBB2 analysis. Here, we performed CMA on 7 
      formalin-fixed paraffin-embedded (FFPE) GEA specimens previously tested by ERBB2 
      fluorescence in situ hybridization (FISH) and evaluated the concordance and 
      performance of CMA. CMA identified 4 (57.1%) samples with amplification of ERBB2, 
      compared to 3 (42.9%) by FISH. CMA also detected several additional DNA copy 
      number variants in these samples, which may have prognostic and therapeutic 
      indications. Further case studies and clinical trials may provide evidence for 
      the utility of CMA-based genomic studies in the management of patients with 
      suspected ERBB2-positive gastroesophageal adenocarcinoma.
FAU - Yu, Alexander
AU  - Yu A
AD  - School of Medicine, University of Texas Medical Branch, Galveston, TX, USA.
FAU - Luikart, Shelby
AU  - Luikart S
AD  - School of Medicine, University of Texas Medical Branch, Galveston, TX, USA.
FAU - Huang, Gengming
AU  - Huang G
AD  - Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
FAU - Han, Song
AU  - Han S
AD  - Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
FAU - Zhao, Jianping
AU  - Zhao J
AD  - Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
FAU - Soong, Lynn
AU  - Soong L
AD  - Department of Microbiology and Immunology, Department of Pathology, University of 
      Texas Medical Branch, Galveston, TX, USA.
FAU - Dong, Jianli
AU  - Dong J
AD  - Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA.
LA  - eng
GR  - T35 AI078878/AI/NIAID NIH HHS/United States
GR  - TL1 TR001440/TR/NCATS NIH HHS/United States
PT  - Journal Article
DEP - 20201107
PL  - India
TA  - Cancer Med J
JT  - Cancer medicine journal
JID - 101744997
PMC - PMC7790124
MID - NIHMS1657199
OTO - NOTNLM
OT  - CMA
OT  - ERBB2
OT  - FISH
OT  - GEA
EDAT- 2021/01/12 06:00
MHDA- 2021/01/12 06:01
PMCR- 2021/01/07
CRDT- 2021/01/11 05:44
PHST- 2021/01/11 05:44 [entrez]
PHST- 2021/01/12 06:00 [pubmed]
PHST- 2021/01/12 06:01 [medline]
PHST- 2021/01/07 00:00 [pmc-release]
PST - ppublish
SO  - Cancer Med J. 2020;3(Suppl 1):39-46. Epub 2020 Nov 7.