PMID- 33428135
OWN - NLM
STAT- MEDLINE
DCOM- 20210924
LR  - 20210924
IS  - 2523-899X (Electronic)
IS  - 2523-899X (Linking)
VI  - 40
IP  - 6
DP  - 2020 Dec
TI  - Promising Effects of Zerumbone on the Regulation of Tumor-promoting Cytokines 
      Induced by TNF-alpha-activated Fibroblasts.
PG  - 1075-1084
LID - 10.1007/s11596-020-2289-7 [doi]
AB  - Inflammation plays an important role in the development of several cancers. 
      Inflammatory cytokines, including tumor necrosis factor-alpha (TNF-alpha), are associated 
      with the induction of inflammation. Chronic inflammation contributes to the 
      progression of cancer through several mechanisms, including increased cytokine 
      production and activation of transcription factors, such as nuclear factor-kappaB 
      (NF-kappaB). Zerumbone (ZER), a component of subtropical ginger (Zingiber zerumbet 
      Smith), seems to have anti-inflammatory, anti-cancer, and antioxidant activities. 
      In this study, we aimed to explore the protective function and mechanisms of ZER 
      against TNF-alpha-induced cancer-promoting cytokines. We found that the viability of 
      stimulated human fibroblast cell lines was reduced after treatment with ZER 
      (IC(50)=18 micromol/L), compared to un-stimulated fibroblasts (IC(50)=40 micromol/L). 
      Besides, ZER inhibited mRNA expression and protein secretion of transforming 
      growth factor-beta (TGF-beta), interleukin-33 (IL-33), monocyte chemoattractant 
      protein-1 (MCP-1), and stromal cell-derived factor 1 (SDF-1), which were produced 
      by TNF-alpha-induced fibroblasts, as measured by quantitative real time-PCR (qRT-PCR) 
      and ELISA assays. The mRNA expression levels of TGF-beta, IL-33, SDF-1, and MCP-1 
      showed 8, 5, 2.5, and 4-fold reductions, respectively. Moreover, secretion of 
      TGF-beta, IL-33, SDF-1, and MCP-1 was reduced to 3.65+/-0.34 ng/mL, 6.3+/-0.26, 
      1703.6+/-295.2, and 5.02+/-0.18 pg/mL, respectively, compared to the untreated group. 
      In addition, the conditioned media (CM) of TNF-alpha-stimulated fibroblasts increased 
      the NF-kappaB expression in colorectal cancer cell lines (HCT-116 and Sw48), while in 
      the vicinity of ZER, the expression of NF-kappaB was reversed. Considering the 
      significant effects of ZER, this component can be used as an appropriate 
      alternative herbal treatment for cancer-related chronic inflammation.
FAU - Radaei, Zahra
AU  - Radaei Z
AD  - Department of Immunology, School of Medicine, Hamadan University of Medical 
      Sciences, Hamadan, 65178, Iran.
FAU - Zamani, Alireza
AU  - Zamani A
AD  - Department of Immunology, School of Medicine, Hamadan University of Medical 
      Sciences, Hamadan, 65178, Iran.
FAU - Najafi, Rezvan
AU  - Najafi R
AD  - Research Center for Molecular Medicine, Hamadan University of Medical Sciences, 
      Hamadan, 65178, Iran.
FAU - Saidijam, Massoud
AU  - Saidijam M
AD  - Research Center for Molecular Medicine, Hamadan University of Medical Sciences, 
      Hamadan, 65178, Iran.
FAU - Jalilian, Farid Azizi
AU  - Jalilian FA
AD  - Department of Microbiology, School of Medicine, Hamadan University of Medical 
      Sciences, Hamadan, 65178, Iran.
FAU - Ezati, Razieh
AU  - Ezati R
AD  - Institute of Medical Biotechnology, National Institute of Genetic Engineering and 
      Biotechnology, Tehran, 14965, Iran.
FAU - Solgi, Ghasem
AU  - Solgi G
AD  - Department of Immunology, School of Medicine, Hamadan University of Medical 
      Sciences, Hamadan, 65178, Iran.
FAU - Amini, Razieh
AU  - Amini R
AD  - Research Center for Molecular Medicine, Hamadan University of Medical Sciences, 
      Hamadan, 65178, Iran. aminra14@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20210111
PL  - China
TA  - Curr Med Sci
JT  - Current medical science
JID - 101729993
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (CCL2 protein, human)
RN  - 0 (CXCL12 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
RN  - 0 (IL33 protein, human)
RN  - 0 (Interleukin-33)
RN  - 0 (NF-kappa B)
RN  - 0 (Sesquiterpenes)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 471-05-6 (zerumbone)
SB  - IM
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cell Culture Techniques
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Chemokine CCL2/genetics/metabolism
MH  - Chemokine CXCL12/genetics/metabolism
MH  - Culture Media, Conditioned/chemistry
MH  - Cytokines/*genetics/*metabolism
MH  - Fibroblasts/*cytology/drug effects/immunology
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - HCT116 Cells
MH  - Humans
MH  - Interleukin-33/genetics/metabolism
MH  - NF-kappa B/genetics/metabolism
MH  - Sesquiterpenes/*pharmacology
MH  - Transforming Growth Factor beta/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/*adverse effects
OTO - NOTNLM
OT  - activated fibroblasts
OT  - inflammation
OT  - nuclear factor-alphaB (NF-alphaB)
OT  - tumor necrosis factor-alpha (TNF-alpha)
OT  - zerumbone
EDAT- 2021/01/12 06:00
MHDA- 2021/09/25 06:00
CRDT- 2021/01/11 12:21
PHST- 2019/05/23 00:00 [received]
PHST- 2019/11/26 00:00 [accepted]
PHST- 2021/01/11 12:21 [entrez]
PHST- 2021/01/12 06:00 [pubmed]
PHST- 2021/09/25 06:00 [medline]
AID - 10.1007/s11596-020-2289-7 [pii]
AID - 10.1007/s11596-020-2289-7 [doi]
PST - ppublish
SO  - Curr Med Sci. 2020 Dec;40(6):1075-1084. doi: 10.1007/s11596-020-2289-7. Epub 2021 
      Jan 11.