PMID- 33428377
OWN - NLM
STAT- MEDLINE
DCOM- 20210302
LR  - 20210302
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 13
IP  - 3
DP  - 2021 Jan 27
TI  - Reaction between Graphene Oxide and Intracellular Glutathione Affects Cell 
      Viability and Proliferation.
PG  - 3528-3535
LID - 10.1021/acsami.0c17523 [doi]
AB  - Graphene oxide (GO) is currently developed for biomedical applications as a 
      promising nanoplatform for drug delivery, phototherapy, and biosensing. As a 
      consequence, its safety and cytotoxicity issues have attracted extensive 
      attention. It has been demonstrated that GO causes an increase of intracellular 
      oxidative stress, likely leading to its cytotoxicity and inhibition of cell 
      proliferation. Being one of the main reductive intracellular substances, 
      glutathione (GSH) is vital in the regulation of the oxidative stress level to 
      maintain normal cellular functions. In this study, we found that GSH could be 
      oxidized to GSSG by GO, leading to the formation of reduced GO (rGO). GSH 
      depletion affects the intracellular reductive/oxidative balance, provoking the 
      increase of the reactive oxygen species level, sequentially inhibiting cell 
      viability and proliferation. Therefore, the reaction between GO and GSH provides 
      a new perspective to explain the origin of GO cytotoxicity.
FAU - Ma, Baojin
AU  - Ma B
AD  - CNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR3572, University 
      of Strasbourg, ISIS, Strasbourg 67000, France.
FAU - Guo, Shi
AU  - Guo S
AD  - CNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR3572, University 
      of Strasbourg, ISIS, Strasbourg 67000, France.
FAU - Nishina, Yuta
AU  - Nishina Y
AUID- ORCID: 0000-0002-4958-1753
AD  - Graduate School of Natural Science and Technology, Okayama University, 
      Tsushimanaka, Kita-ku, Okayama 700-8530, Japan.
AD  - Research Core for Interdisciplinary Sciences, Okayama University, Tsushimanaka, 
      Kita-ku, Okayama 700-8530, Japan.
FAU - Bianco, Alberto
AU  - Bianco A
AUID- ORCID: 0000-0002-1090-296X
AD  - CNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR3572, University 
      of Strasbourg, ISIS, Strasbourg 67000, France.
LA  - eng
PT  - Journal Article
DEP - 20210111
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Biocompatible Materials)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (graphene oxide)
RN  - 7782-42-5 (Graphite)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Biocompatible Materials/metabolism/*toxicity
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/*drug effects
MH  - Glutathione/*metabolism
MH  - Graphite/metabolism/*toxicity
MH  - HeLa Cells
MH  - Humans
MH  - Oxidative Stress/*drug effects
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - cancer cells
OT  - carbon nanomaterial
OT  - oxidation
OT  - reactive oxygen species
OT  - reduction
EDAT- 2021/01/12 06:00
MHDA- 2021/03/03 06:00
CRDT- 2021/01/11 17:09
PHST- 2021/01/12 06:00 [pubmed]
PHST- 2021/03/03 06:00 [medline]
PHST- 2021/01/11 17:09 [entrez]
AID - 10.1021/acsami.0c17523 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2021 Jan 27;13(3):3528-3535. doi: 
      10.1021/acsami.0c17523. Epub 2021 Jan 11.