PMID- 33434702
OWN - NLM
STAT- MEDLINE
DCOM- 20220107
LR  - 20220107
IS  - 1567-7257 (Electronic)
IS  - 1567-1348 (Linking)
VI  - 89
DP  - 2021 Apr
TI  - Bioinformatics features and immunogenic epitopes of Echinococcus granulosus 
      Myophilin as a promising target for vaccination against cystic echinococcosis.
PG  - 104714
LID - S1567-1348(21)00011-3 [pii]
LID - 10.1016/j.meegid.2021.104714 [doi]
AB  - Cystic echinococcosis (CE) is a neglected zoonosis especially in underdeveloped 
      countries around the world. Hence, immunization strategies are beneficial to 
      avert the infection. The present investigation was aimed to predict the primary 
      biochemical characteristics of the EgMyophilin and its potential B-cell and human 
      leukocyte antigen (HLA)-binding epitopes as a promising vaccine candidate. 
      Different web servers were used to predict physico-chemical, antigenic and 
      allergenic profiles, transmembrane domain, subcellular localization, 
      post-translational modification (PTM) sites, secondary and 3D structure, tertiary 
      model refinement and validations. B-cell and HLA-binding epitopes were predicted 
      and screened in terms antigenicity, allergenicity, solubility (B-cell) or 
      hydrophobicity (T-cell). The 89.82 KDa protein was non-allergenic, hydrophilic, 
      stable, with improved thermotolerance and 94 post-translational modification 
      sites. The secondary structure included 42.94% alpha helix, 42.82% random coil 
      and 41.23% extended strand. Based on Ramachandran plot output for refined model, 
      96.2%, 99.5%, and 0.45% of amino acid residues were incorporated in the favored, 
      allowed, and outlier regions of the refined model, respectively. After epitope 
      screening, four B-cell and five HLA-binding epitopes possessed the highest 
      antigenic index in the protein sequence. This paper is a premise for further 
      researches, and provides insights for the development of a suitable vaccine 
      against CE. More empirical studies are required using the EgMyophilin alone or in 
      combination with other antigens/epitopes in the future.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Shams, Morteza
AU  - Shams M
AD  - Zoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, 
      Iran.
FAU - Javanmardi, Erfan
AU  - Javanmardi E
AD  - Clinical Research Development Center, "The Persian Gulf Martyrs" Hospital, 
      Bushehr, University of Medical Sciences, Bushehr, Iran.
FAU - Nosrati, Mohammadreza Chaechi
AU  - Nosrati MC
AD  - Department of Pathobiology, Science and Research Branch, Islamic Azad University, 
      Tehran, Iran.
FAU - Ghasemi, Ezatollah
AU  - Ghasemi E
AD  - Department of Medical Parasitology, School of Medicine, Dezful University of 
      Medical Sciences, Dezful, Iran.
FAU - Shamsinia, Sadegh
AU  - Shamsinia S
AD  - Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Yousefi, Ali
AU  - Yousefi A
AD  - Students Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
FAU - Kordi, Bahareh
AU  - Kordi B
AD  - Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, 
      Neyshabur, Iran; Department of Pathobiology, Faculty of Veterinary Medicine, 
      Ferdowsi University of Mashhad, Mashhad, Iran.
FAU - Majidiani, Hamidreza
AU  - Majidiani H
AD  - Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, 
      Neyshabur, Iran. Electronic address: H.majidiani@modares.ac.ir.
FAU - Nourmohammadi, Hassan
AU  - Nourmohammadi H
AD  - Zoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, 
      Iran; Department of Internal Medicine, Shahid Mostafa Khomeini Hospital, Ilam 
      University of Medical Sciences, Ilam, Iran. Electronic address: 
      Nourmohammadi-H@medilam.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20210109
PL  - Netherlands
TA  - Infect Genet Evol
JT  - Infection, genetics and evolution : journal of molecular epidemiology and 
      evolutionary genetics in infectious diseases
JID - 101084138
RN  - 0 (Antigens, Helminth)
RN  - 0 (Epitopes)
RN  - 0 (Protozoan Vaccines)
SB  - IM
MH  - Animals
MH  - Antigens, Helminth/immunology
MH  - Computational Biology/*methods
MH  - Echinococcosis/*prevention & control
MH  - Echinococcus granulosus/*immunology
MH  - Epitopes/*immunology
MH  - Humans
MH  - Protozoan Vaccines/*immunology
OTO - NOTNLM
OT  - Echinococcus granulosus
OT  - Immunoinformatics
OT  - Myophilin
OT  - Vaccination
EDAT- 2021/01/13 06:00
MHDA- 2022/01/08 06:00
CRDT- 2021/01/12 20:09
PHST- 2020/10/16 00:00 [received]
PHST- 2020/11/27 00:00 [revised]
PHST- 2021/01/06 00:00 [accepted]
PHST- 2021/01/13 06:00 [pubmed]
PHST- 2022/01/08 06:00 [medline]
PHST- 2021/01/12 20:09 [entrez]
AID - S1567-1348(21)00011-3 [pii]
AID - 10.1016/j.meegid.2021.104714 [doi]
PST - ppublish
SO  - Infect Genet Evol. 2021 Apr;89:104714. doi: 10.1016/j.meegid.2021.104714. Epub 
      2021 Jan 9.