PMID- 33435676
OWN - NLM
STAT- MEDLINE
DCOM- 20210302
LR  - 20240226
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 13
IP  - 3
DP  - 2021 Jan 27
TI  - Sandwich-Type Near-Infrared Conjugated Polymer Nanoparticles for Revealing the 
      Fate of Transplanted Human Umbilical Cord Mesenchymal Stem Cells.
PG  - 3512-3520
LID - 10.1021/acsami.0c13815 [doi]
AB  - Near-infrared conjugated polymer nanoparticles (NIR-CPNs) have been widely used 
      in in vivo imaging fields. However, most of them face the aggregation-induced 
      fluorescence quenching (ACQ) dilemma and serious dye leakage behavior, which 
      impedes the long-term monitoring of transplanted cells in vivo. In the present 
      work, a novel strategy of sandwich-type encapsulation of the conjugated polymer 
      interlayer in the crystalline SiO(2) core + shell ((S)SiO(2)@(S)PFTBT@(C)SiO(2)) 
      is developed, which works well to avoid the ACQ problem by homogeneously 
      dispersing 
      poly((9,9-dioctylfluorene-2,7-diyl)-alt-(4,7-di(thiophene-2-yl)-2,1,3-benzothiadiazole)-5',5''-diyl) 
      (PFTBT) and suppressing intermolecular pi-pi stacking. Furthermore, the 
      unparalleled nanostructure efficiently stabilizes nanoparticles and successfully 
      achieves long-term biocompatibility without interfering the biological 
      characteristics of stem cells, indicating the potential of 
      (S)SiO(2)@(S)PFTBT@(C)SiO(2) in cell labeling. In addition, the fate of human 
      umbilical cord mesenchymal stem cells (hucMSCs) in a mouse model with acute liver 
      injury was disclosed. We found that the hucMSCs mainly migrated from the lungs to 
      the injured liver and most transplanted hucMSCs were cleared up by the liver at 8 
      days post-injection. Revelation of the shuttle process and period will benefit in 
      improving the clinical efficacy of hucMSCs, and the sandwich-type encapsulation 
      strategy could also open a new avenue to obtain bright and robust NIR-CPNs for 
      long-term fluorescence imaging.
FAU - Zhang, Junhong
AU  - Zhang J
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
FAU - Mohsin, Ali
AU  - Mohsin A
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
FAU - Peng, Yan
AU  - Peng Y
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
FAU - Dai, Yichen
AU  - Dai Y
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
FAU - Zhuang, Yingping
AU  - Zhuang Y
AUID- ORCID: 0000-0002-6772-7306
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
FAU - Guo, Meijin
AU  - Guo M
AUID- ORCID: 0000-0002-3171-4802
AD  - State Key Laboratory of Bioreactor Engineering, East China University of Science 
      and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
FAU - Zhao, Peng
AU  - Zhao P
AUID- ORCID: 0000-0003-2380-2869
AD  - School of Chemistry and Chemical Engineering, State Key Laboratory of Metal 
      Matrix Composites, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 
      200240, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20210113
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Polymers)
RN  - 0 (Thiadiazoles)
RN  - 0 (Thiophenes)
RN  - 25233-34-5 (polythiophene)
RN  - 273-77-8 (benzo-1,2,3-thiadiazole)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Acute Lung Injury/diagnostic imaging/therapy
MH  - Animals
MH  - Cell Line
MH  - Cell Tracking/*methods
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice, Inbred BALB C
MH  - Nanoparticles/*chemistry
MH  - Optical Imaging/*methods
MH  - Polymers/*chemistry
MH  - Silicon Dioxide/chemistry
MH  - Thiadiazoles/chemistry
MH  - Thiophenes/*chemistry
MH  - Umbilical Cord/cytology
MH  - Mice
OTO - NOTNLM
OT  - NIR fluorescence
OT  - conjugated polymer nanoparticles
OT  - hucMSCs
OT  - in vivo imaging
OT  - sandwich-type encapsulation
EDAT- 2021/01/14 06:00
MHDA- 2021/03/03 06:00
CRDT- 2021/01/13 05:40
PHST- 2021/01/14 06:00 [pubmed]
PHST- 2021/03/03 06:00 [medline]
PHST- 2021/01/13 05:40 [entrez]
AID - 10.1021/acsami.0c13815 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2021 Jan 27;13(3):3512-3520. doi: 
      10.1021/acsami.0c13815. Epub 2021 Jan 13.