PMID- 33440936
OWN - NLM
STAT- MEDLINE
DCOM- 20210423
LR  - 20210423
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 55
IP  - 3
DP  - 2021 Feb 2
TI  - Comammox Nitrospira Species Dominate in an Efficient Partial 
      Nitrification-Anammox Bioreactor for Treating Ammonium at Low Loadings.
PG  - 2087-2098
LID - 10.1021/acs.est.0c05777 [doi]
AB  - Bacteria capable of complete ammonia oxidation (comammox) are widespread and 
      contribute to nitrification in wastewater treatment facilities. However, their 
      roles in partial nitrification-anaerobic ammonium oxidation (anammox) systems 
      remain unclear. In this study, a bench-scale bioreactor with continuous stirring 
      was operated for more than 1000 days with limited oxygen supply to achieve 
      efficient nitrogen removal (70.1 +/- 2.7%) at a low ammonium loading of 35.2 
      mg-N/L/day. High-throughput amplicon sequencing analysis of the comammox ammonia 
      monooxygenase subunit A (amoA) gene revealed seven sequence types from two 
      clusters in clade A of comammox Nitrospira. Quantitative polymerase chain 
      reaction analyses suggested that the comammox species dominated the 
      ammonia-oxidizing community, with an abundance as high as 89.2 +/- 7.9% in total 
      prokaryotic amoA copies. Multiple linear regression further revealed the 
      substantial contribution of the comammox Nitrospira to ammonia oxidation in the 
      bioreactor. The investigation with bioreactor and batch experiments consistently 
      showed that activities of comammox Nitrospira were inhibited by free ammonia far 
      more severely than other ammonia-oxidizing microbes. Overall, this study provided 
      new insight into the ecology of comammox Nitrospira under hypoxic conditions and 
      suggested comammox-associated partial nitrification-anammox as a potential method 
      for treating low-strength ammonium-containing wastewater.
FAU - Shao, Yung-Hsien
AU  - Shao YH
AD  - Department of Environmental Engineering, National Cheng Kung University, Tainan 
      City 701, Taiwan.
FAU - Wu, Jer-Horng
AU  - Wu JH
AUID- ORCID: 0000-0003-4926-2995
AD  - Department of Environmental Engineering, National Cheng Kung University, Tainan 
      City 701, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210113
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Ammonium Compounds)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - Ammonia
MH  - *Ammonium Compounds
MH  - Bacteria/genetics
MH  - Bioreactors
MH  - *Nitrification
MH  - Oxidation-Reduction
MH  - Phylogeny
EDAT- 2021/01/15 06:00
MHDA- 2021/04/24 06:00
CRDT- 2021/01/14 05:30
PHST- 2021/01/15 06:00 [pubmed]
PHST- 2021/04/24 06:00 [medline]
PHST- 2021/01/14 05:30 [entrez]
AID - 10.1021/acs.est.0c05777 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2021 Feb 2;55(3):2087-2098. doi: 10.1021/acs.est.0c05777. 
      Epub 2021 Jan 13.