PMID- 33441634
OWN - NLM
STAT- MEDLINE
DCOM- 20210817
LR  - 20231104
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Jan 13
TI  - Melatonin administration provokes the activity of dendritic reticular cells in 
      the seminal vesicle of Soay ram during the non-breeding season.
PG  - 872
LID - 10.1038/s41598-020-79529-y [doi]
LID - 872
AB  - Dendritic cells (DCs) are innate immune cells which engulf, process and present 
      antigens to the naive T-lymphocyte cells. However, little is known about the 
      effect of melatonin on the DCs. The present study aimed to investigate the 
      morphology and distribution of the DCs by transmission electron microscopy and 
      Immunohistochemistry after melatonin administration. A total of 8 out of 15 adult 
      ram was randomly selected to receive the melatonin implant and the remaining 7 
      animals received melatonin free implants. DCs showed positive immunoreactivity 
      for CD117, S-100 protein and CD34. There is an obvious increase in the number of 
      the positive immunoreactive cells to CD3, estrogen receptor alpha and 
      progesterone in the treated groups. The expression of CD56 and MHCII in the DCs 
      was abundant in the treated groups. The ultrastructure study revealed that 
      melatonin exerts a stimulatory effect on the DCs which was associated with 
      increment in the secretory activity of DCs. The secretory activity demarcated by 
      an obvious increase in the number of mitochondria, cisternae of rER and a 
      well-developed Golgi apparatus. The endosomal- lysosomal system was more 
      developed in the treated groups. A rod-shaped Birbeck granule was demonstrated in 
      the cytoplasm of the melatonin treated group. DCs were observed in a close 
      contact to telocytes, T-Lymphocytes, nerve fibers and blood vessels. Taken 
      together, melatonin administration elicits a stimulatory action on the DCs and 
      macrophages through increasing the size, the number and the endosomal 
      compartments which may correlate to increased immunity.
FAU - Abd-Elhafeez, Hanan H
AU  - Abd-Elhafeez HH
AUID- ORCID: 0000-0002-2547-0709
AD  - Department of Anatomy, Embryology and Histology, Faculty of Veterinary Medicine, 
      Assiut University, Assiut, 71526, Egypt.
FAU - Hassan, A H S
AU  - Hassan AHS
AD  - Department of Anatomy, Embryology and Histology, Faculty of Veterinary Medicine, 
      Assiut University, Assiut, 71526, Egypt.
FAU - Hussein, Manal T
AU  - Hussein MT
AUID- ORCID: 0000-0003-2801-6066
AD  - Department of Anatomy, Embryology and Histology, Faculty of Veterinary Medicine, 
      Assiut University, Assiut, 71526, Egypt. manal.hussein@aun.edu.eg.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210113
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Animals
MH  - Dendritic Cells/drug effects/metabolism
MH  - Dendritic Cells, Follicular/drug effects/*metabolism
MH  - Lymphocytes/immunology
MH  - Macrophages/drug effects/metabolism
MH  - Male
MH  - Melatonin/metabolism/*pharmacology
MH  - Seminal Vesicles/drug effects/*metabolism
MH  - Sheep
MH  - T-Lymphocytes/drug effects/immunology
MH  - Telocytes/drug effects/immunology
PMC - PMC7806754
COIS- The authors declare no competing interests.
EDAT- 2021/01/15 06:00
MHDA- 2021/08/18 06:00
PMCR- 2021/01/13
CRDT- 2021/01/14 05:38
PHST- 2020/03/17 00:00 [received]
PHST- 2020/12/09 00:00 [accepted]
PHST- 2021/01/14 05:38 [entrez]
PHST- 2021/01/15 06:00 [pubmed]
PHST- 2021/08/18 06:00 [medline]
PHST- 2021/01/13 00:00 [pmc-release]
AID - 10.1038/s41598-020-79529-y [pii]
AID - 79529 [pii]
AID - 10.1038/s41598-020-79529-y [doi]
PST - epublish
SO  - Sci Rep. 2021 Jan 13;11(1):872. doi: 10.1038/s41598-020-79529-y.