PMID- 33441834
OWN - NLM
STAT- MEDLINE
DCOM- 20210811
LR  - 20210811
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Jan 13
TI  - Transcript levels of keratin 1/5/6/14/15/16/17 as potential prognostic indicators 
      in melanoma patients.
PG  - 1023
LID - 10.1038/s41598-020-80336-8 [doi]
LID - 1023
AB  - Keratins (KRTs), the intermediate filament-forming proteins of epithelial cells, 
      are extensively used as diagnostic biomarkers in cancers and associated with 
      tumorigenesis and metastasis in multiple cancers. However, the diverse expression 
      patterns and prognostic values of KRTs in melanoma have yet to be elucidated. In 
      the current study, we examined the transcriptional and clinical data of KRTs in 
      patients with melanoma from GEO, TCGA, ONCOMINE, GEPIA, cBioPortal, TIMER and 
      TISIDB databases. We found that the mRNA levels of KRT1/2/5/6/8/10/14/15/16/17 
      were significantly differential expressed between primary melanoma and metastatic 
      melanoma. The expression levels of KRT1/2/5/6/10/14/15/16/17 were correlated with 
      advanced tumor stage. Survival analysis revealed that the high transcription 
      levels of KRT1/5/6/14/15/16/17 were associated with low overall survival in 
      melanoma patients. GSEA analysis indicated that the most involved hallmarks 
      pathways were P53 pathway, KRAS signaling, estrogen response early and estrogen 
      response late. Furthermore, we found some correlations among the expression of 
      KRTs and the infiltration of immune cells. Our study may provide novel insights 
      for the selection of prognostic biomarkers for melanoma.
FAU - Han, Wei
AU  - Han W
AD  - Department of Burn and Plastic Surgery, The First Affiliated Hospital of Soochow 
      University, No. 188 Shizi Street, Suzhou, 215000, People's Republic of China.
AD  - Department of Surgery, Soochow University, Suzhou, 215000, People's Republic of 
      China.
FAU - Hu, Chan
AU  - Hu C
AD  - Institute of Photomedicine, Shanghai Skin Disease Hospital, Tongji University 
      School of Medicine, Shanghai, 200000, People's Republic of China.
FAU - Fan, Zhao-Jun
AU  - Fan ZJ
AD  - Department of Burn and Plastic Surgery, The First Affiliated Hospital of Soochow 
      University, No. 188 Shizi Street, Suzhou, 215000, People's Republic of China.
AD  - Department of Surgery, Soochow University, Suzhou, 215000, People's Republic of 
      China.
FAU - Shen, Guo-Liang
AU  - Shen GL
AD  - Department of Burn and Plastic Surgery, The First Affiliated Hospital of Soochow 
      University, No. 188 Shizi Street, Suzhou, 215000, People's Republic of China. 
      sdfyysgl@163.com.
AD  - Department of Surgery, Soochow University, Suzhou, 215000, People's Republic of 
      China. sdfyysgl@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210113
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RNA, Messenger)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Databases, Nucleic Acid
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Regulatory Networks
MH  - Humans
MH  - Kaplan-Meier Estimate
MH  - Keratins/classification/*genetics/metabolism
MH  - Melanoma/*genetics/metabolism/secondary
MH  - Mutation
MH  - Prognosis
MH  - Protein Interaction Maps/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Signal Transduction/genetics
MH  - Skin Neoplasms/*genetics/metabolism/pathology
PMC - PMC7806772
COIS- The authors declare no competing interests.
EDAT- 2021/01/15 06:00
MHDA- 2021/08/12 06:00
PMCR- 2021/01/13
CRDT- 2021/01/14 05:41
PHST- 2020/04/25 00:00 [received]
PHST- 2020/12/10 00:00 [accepted]
PHST- 2021/01/14 05:41 [entrez]
PHST- 2021/01/15 06:00 [pubmed]
PHST- 2021/08/12 06:00 [medline]
PHST- 2021/01/13 00:00 [pmc-release]
AID - 10.1038/s41598-020-80336-8 [pii]
AID - 80336 [pii]
AID - 10.1038/s41598-020-80336-8 [doi]
PST - epublish
SO  - Sci Rep. 2021 Jan 13;11(1):1023. doi: 10.1038/s41598-020-80336-8.