PMID- 33442254
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220419
IS  - 1176-6328 (Print)
IS  - 1178-2021 (Electronic)
IS  - 1176-6328 (Linking)
VI  - 17
DP  - 2021
TI  - MicroRNA-488-3p Regulates Neuronal Cell Death in Cerebral Ischemic Stroke Through 
      Vacuolar Protein Sorting 4B (VPS4B).
PG  - 41-55
LID - 10.2147/NDT.S255666 [doi]
AB  - BACKGROUND: Ischemic stroke, which often occurs with high morbidity, disability, 
      and mortality, is a main cause of brain disease. In various types of human 
      diseases, it is found that microRNAs (miRNAs) are considered as gene regulators. 
      Increasing studies have proved that fluctuation of miRNAs, in the pathologies of 
      ischemic stroke, plays a vital role. However, the accurate regulatory mechanism 
      of cerebral ischemic stroke by miRNAs is still unclear. In this research, we 
      investigated the inhibition mechanism of miR-488-3p on neuronal death through 
      targeting vacuolar protein sorting 4B (VPS4B) in cerebral ischemia/reperfusion 
      (I/R) injury. METHODS: Western blot and qRT-PCR were utilized to detect the 
      miR-488-3p level and VPS4B expression. The cell counting kit-8 (CCK-8) assay was 
      utilized to measure the function of miR-488-3p in cell death induced by oxygen 
      glucose deprivation/reoxygenation (OGD/R). After middle cerebral artery 
      occlusion/reperfusion (MCAO/R), the impact of miR-488-3p on infarct volume in 
      mouse brain was assessed. The targets of miR-488-3p were confirmed by luciferase 
      analysis and bioinformatics software. RESULTS: The miR-488-3p level remarkably 
      reduced in primary neuronal cells administrated with OGD/R. Similarly, it also 
      decreased in the mouse brain administrated with MCAO/R. Additionally, the 
      up-regulation of miR-488-3p expression suppressed the death of neuronal cells and 
      restrained ischemic brain infarction in ischemia-stroked mice. Besides, the 
      results showed that VPS4B, which could be inhibited by miR-488-3p, was a direct 
      target of miR-488-3p. This research revealed that the inhibition of VPS4B 
      protected the neuronal cells in ischemic stroke both in vitro as well as in vivo. 
      Meanwhile, this inhibition strengthened positive impact generated by miR-488-3p 
      on ischemic injury. CONCLUSION: Overall, miR-488-3p played a critical role on 
      neuroprotective function via reducing VPS4B protein level. These results 
      performed a new underlying curative target for the treatment of cerebral ischemic 
      stroke.
CI  - (c) 2021 Zhou et al.
FAU - Zhou, Li
AU  - Zhou L
AD  - Department of Rehabilitation, The First Affiliated Hospital of Guangdong 
      Pharmaceutical University, Guangzhou 510080, People's Republic of China.
FAU - Yang, Wanxin
AU  - Yang W
AD  - Department of Rehabilitation, The First Affiliated Hospital of Guangdong 
      Pharmaceutical University, Guangzhou 510080, People's Republic of China.
FAU - Yao, Enping
AU  - Yao E
AD  - Department of Rehabilitation, The First Affiliated Hospital of Guangdong 
      Pharmaceutical University, Guangzhou 510080, People's Republic of China.
FAU - Li, Haiyan
AU  - Li H
AD  - Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, 
      Guangzhou 510000, People's Republic of China.
FAU - Wang, Jihui
AU  - Wang J
AD  - Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, 
      Guangzhou 510000, People's Republic of China.
FAU - Wang, Kun
AU  - Wang K
AD  - School of Health Science, Guangdong Pharmaceutical University, Guangzhou 510310, 
      People's Republic of China.
FAU - Zhong, Xiaohua
AU  - Zhong X
AD  - Department of Rehabilitation, The First Affiliated Hospital of Guangdong 
      Pharmaceutical University, Guangzhou 510080, People's Republic of China.
FAU - Peng, Zhongxing
AU  - Peng Z
AD  - Department of Neurology, The First Affiliated Hospital of Guangdong 
      Pharmaceutical University, Guangzhou 510080, People's Republic of China.
FAU - Huang, Xuming
AU  - Huang X
AD  - Department of Rehabilitation, The First Affiliated Hospital of Guangdong 
      Pharmaceutical University, Guangzhou 510080, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20210107
PL  - New Zealand
TA  - Neuropsychiatr Dis Treat
JT  - Neuropsychiatric disease and treatment
JID - 101240304
PMC - PMC7800712
OTO - NOTNLM
OT  - I/R
OT  - VPS4B
OT  - ischemia/reperfusion
OT  - ischemic stroke
OT  - miR-488-3p
COIS- The authors report no conflicts of interest in this work.
EDAT- 2021/01/15 06:00
MHDA- 2021/01/15 06:01
PMCR- 2021/01/07
CRDT- 2021/01/14 05:46
PHST- 2020/03/26 00:00 [received]
PHST- 2020/11/23 00:00 [accepted]
PHST- 2021/01/14 05:46 [entrez]
PHST- 2021/01/15 06:00 [pubmed]
PHST- 2021/01/15 06:01 [medline]
PHST- 2021/01/07 00:00 [pmc-release]
AID - 255666 [pii]
AID - 10.2147/NDT.S255666 [doi]
PST - epublish
SO  - Neuropsychiatr Dis Treat. 2021 Jan 7;17:41-55. doi: 10.2147/NDT.S255666. 
      eCollection 2021.