PMID- 33443415
OWN - NLM
STAT- MEDLINE
DCOM- 20210423
LR  - 20210423
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 55
IP  - 3
DP  - 2021 Feb 2
TI  - Robust Nitritation of Anaerobic Digester Centrate Using Dual Stressors and Timed 
      Alkali Additions.
PG  - 2016-2026
LID - 10.1021/acs.est.0c04613 [doi]
AB  - Nitrogen is commonly removed from wastewater by nitrification to nitrate followed 
      by nitrate reduction to N(2). Shortcut N removal saves energy by limiting ammonia 
      oxidation to nitrite, but nitrite accumulation can be unstable. We hypothesized 
      that repeated short-term exposures of ammonia-oxidizing communities to free 
      ammonia (FA) and free nitrous acid (FNA) would stabilize nitritation by selecting 
      against nitrite-oxidizing bacteria (NOB). Accordingly, we evaluated ammonium 
      oxidation of anaerobic digester centrate in two bench-scale sequencing batch 
      reactors (SBRs), seeded with the same inoculum and operated identically but with 
      differing pH-control strategies. A single stressor SBR (SS/SBR) using pH 
      set-point control produced HNO(3), while a dual stressor SBR (DS/SBR) using timed 
      alkalinity addition (TAA) produced HNO(2) (ammonium removal efficiency of 97 +/- 
      2%; nitrite accumulation ratio of 98 +/- 1%). The TAA protocol was developed during 
      an adaptation period with continuous pH monitoring. After adaptation, automated 
      TAA enabled stable nitritation without set-point control. In the SS/SBR, 
      repeatedly exposing the community to FA (8-10 h/exposure, one exposure/cycle) 
      selected for FA-tolerant ammonia-oxidizing bacteria (Nitrosomonas sp. NM107) and 
      NOB (Nitrobacter sp.). In the DS/SBR, repeatedly exposing the community to FA 
      (2-4 h/exposure, three exposures/cycle) and FNA (4-6 h/exposure, two 
      exposures/cycle) selected for FA- and FNA-resistant AOB (Nitrosomonas IWT514) and 
      against NOB, stabilizing nitritation.
FAU - Yao, Yinuo
AU  - Yao Y
AUID- ORCID: 0000-0001-8328-6072
AD  - Department of Civil and Environmental Engineering, Stanford University, Stanford, 
      California 94305, United States.
FAU - Wang, Zhiyue
AU  - Wang Z
AUID- ORCID: 0000-0002-0776-050X
AD  - Department of Civil and Environmental Engineering, Stanford University, Stanford, 
      California 94305, United States.
FAU - Criddle, Craig S
AU  - Criddle CS
AUID- ORCID: 0000-0002-2750-8547
AD  - Department of Civil and Environmental Engineering, Stanford University, Stanford, 
      California 94305, United States.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20210114
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Alkalies)
RN  - 0 (Nitrites)
RN  - 7664-41-7 (Ammonia)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - *Alkalies
MH  - Ammonia
MH  - Anaerobiosis
MH  - *Bioreactors
MH  - Nitrification
MH  - Nitrites
MH  - Nitrobacter
MH  - Nitrogen
MH  - Oxidation-Reduction
EDAT- 2021/01/15 06:00
MHDA- 2021/04/24 06:00
CRDT- 2021/01/14 12:08
PHST- 2021/01/15 06:00 [pubmed]
PHST- 2021/04/24 06:00 [medline]
PHST- 2021/01/14 12:08 [entrez]
AID - 10.1021/acs.est.0c04613 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2021 Feb 2;55(3):2016-2026. doi: 10.1021/acs.est.0c04613. 
      Epub 2021 Jan 14.