PMID- 33445473
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210126
IS  - 2076-2615 (Print)
IS  - 2076-2615 (Electronic)
IS  - 2076-2615 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Jan 12
TI  - Whole Genome Sequencing Reveals the Effects of Recent Artificial Selection on 
      Litter Size of Bamei Mutton Sheep.
LID - 10.3390/ani11010157 [doi]
LID - 157
AB  - Bamei mutton sheep is a Chinese domestic sheep breed developed by crossing German 
      Mutton Merino sheep and indigenous Mongolian sheep for meat production. Here, we 
      focused on detecting candidate genes associated with the increasing of the litter 
      size in this breeds under recent artificial selection to improve the efficiency 
      of mutton production. We selected five high- and five low-fecundity Bamei mutton 
      sheep for whole-genome resequencing to identify candidate genes for sheep 
      prolificacy. We used the FST and XP-EHH statistical approach to detect the 
      selective sweeps between these two groups. Combining the two selective sweep 
      methods, the reproduction-related genes JUN, ITPR3, PLCB2, HERC5, and KDM4B were 
      detected. JUN, ITPR3, and PLCB2 play vital roles in GnRH (gonadotropin-releasing 
      hormone), oxytocin, and estrogen signaling pathway. Moreover, KDM4B, which had 
      the highest FST value, exhibits demethylase activity. It can affect reproduction 
      by binding the promoters of estrogen-regulated genes, such as FOXA1 (forkhead box 
      A1) and ESR1 (estrogen receptor 1). Notably, one nonsynonymous mutation (p.S936A) 
      specific to the high-prolificacy group was identified at the TUDOR domain of 
      KDM4B. These observations provide a new opportunity to research the genetic 
      variation influencing fecundity traits within a population evolving under 
      artificial selection. The identified genomic regions that are responsible for 
      litter size can in turn be used for further selection.
FAU - Yao, Yaxin
AU  - Yao Y
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of 
      Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of 
      Agricultural Sciences, Beijing 100193, China.
FAU - Pan, Zhangyuan
AU  - Pan Z
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of 
      Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of 
      Agricultural Sciences, Beijing 100193, China.
FAU - Di, Ran
AU  - Di R
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of 
      Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of 
      Agricultural Sciences, Beijing 100193, China.
FAU - Liu, Qiuyue
AU  - Liu Q
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of 
      Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of 
      Agricultural Sciences, Beijing 100193, China.
FAU - Hu, Wenping
AU  - Hu W
AUID- ORCID: 0000-0002-8962-7541
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of 
      Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of 
      Agricultural Sciences, Beijing 100193, China.
FAU - Guo, Xiaofei
AU  - Guo X
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of 
      Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of 
      Agricultural Sciences, Beijing 100193, China.
AD  - Tianjin Institute of Animal Sciences, Tianjin 300381, China.
FAU - He, Xiaoyun
AU  - He X
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of 
      Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of 
      Agricultural Sciences, Beijing 100193, China.
FAU - Gan, Shangquan
AU  - Gan S
AD  - State Key Laboratory of Sheep Genetic Improvement and Healthy Production, 
      Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.
FAU - Wang, Xiangyu
AU  - Wang X
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of 
      Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of 
      Agricultural Sciences, Beijing 100193, China.
FAU - Chu, Mingxing
AU  - Chu M
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of 
      Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of 
      Agricultural Sciences, Beijing 100193, China.
LA  - eng
GR  - 2016ZX08009-003-006/qiuyue liu/
PT  - Journal Article
DEP - 20210112
PL  - Switzerland
TA  - Animals (Basel)
JT  - Animals : an open access journal from MDPI
JID - 101635614
PMC - PMC7827510
OTO - NOTNLM
OT  - Bamei mutton sheep
OT  - breeding
OT  - litter size
OT  - selection signal analysis
OT  - whole-genome sequencing
COIS- The authors declare no conflict of interest.
EDAT- 2021/01/16 06:00
MHDA- 2021/01/16 06:01
PMCR- 2021/01/12
CRDT- 2021/01/15 01:01
PHST- 2020/12/07 00:00 [received]
PHST- 2021/01/05 00:00 [revised]
PHST- 2021/01/06 00:00 [accepted]
PHST- 2021/01/15 01:01 [entrez]
PHST- 2021/01/16 06:00 [pubmed]
PHST- 2021/01/16 06:01 [medline]
PHST- 2021/01/12 00:00 [pmc-release]
AID - ani11010157 [pii]
AID - animals-11-00157 [pii]
AID - 10.3390/ani11010157 [doi]
PST - epublish
SO  - Animals (Basel). 2021 Jan 12;11(1):157. doi: 10.3390/ani11010157.