PMID- 33446857
OWN - NLM
STAT- MEDLINE
DCOM- 20210709
LR  - 20240226
IS  - 2399-3642 (Electronic)
IS  - 2399-3642 (Linking)
VI  - 4
IP  - 1
DP  - 2021 Jan 14
TI  - Whole brain dynamics during optogenetic self-stimulation of the medial prefrontal 
      cortex in mice.
PG  - 66
LID - 10.1038/s42003-020-01612-x [doi]
LID - 66
AB  - Intracranial self-stimulation, in which an animal performs an operant response to 
      receive regional brain electrical stimulation, is a widely used procedure to 
      study motivated behavior. While local neuronal activity has long been measured 
      immediately before or after the operant, imaging the whole brain in real-time 
      remains a challenge. Herein we report a method that permits functional MRI (fMRI) 
      of brain dynamics while mice are cued to perform an operant task: licking a spout 
      to receive optogenetic stimulation to the medial prefrontal cortex (MPFC) during 
      a cue ON, but not cue OFF. Licking during cue ON results in activation of a 
      widely distributed network consistent with underlying MPFC projections, while 
      licking during cue OFF (without optogenetic stimulation) leads to negative fMRI 
      signal in brain regions involved in acute extinction. Noninvasive whole brain 
      readout combined with circuit-specific neuromodulation opens an avenue for 
      investigating adaptive behavior in both healthy and disease models.
FAU - Cover, Christopher G
AU  - Cover CG
AD  - Neuroimaging Research Branch, National Institute on Drug Abuse, Intramural 
      Research Program, NIH, Baltimore, MD, 21224, USA.
FAU - Kesner, Andrew J
AU  - Kesner AJ
AD  - Behavioral Neuroscience Research Branch, National Institute on Drug Abuse, 
      Intramural Research Program, NIH, Baltimore, MD, 21224, USA.
FAU - Ukani, Shehzad
AU  - Ukani S
AD  - Neuroimaging Research Branch, National Institute on Drug Abuse, Intramural 
      Research Program, NIH, Baltimore, MD, 21224, USA.
FAU - Stein, Elliot A
AU  - Stein EA
AUID- ORCID: 0000-0003-0593-0269
AD  - Neuroimaging Research Branch, National Institute on Drug Abuse, Intramural 
      Research Program, NIH, Baltimore, MD, 21224, USA.
FAU - Ikemoto, Satoshi
AU  - Ikemoto S
AUID- ORCID: 0000-0002-0732-7386
AD  - Behavioral Neuroscience Research Branch, National Institute on Drug Abuse, 
      Intramural Research Program, NIH, Baltimore, MD, 21224, USA.
FAU - Yang, Yihong
AU  - Yang Y
AD  - Neuroimaging Research Branch, National Institute on Drug Abuse, Intramural 
      Research Program, NIH, Baltimore, MD, 21224, USA. yihongyang@mail.nih.gov.
FAU - Lu, Hanbing
AU  - Lu H
AUID- ORCID: 0000-0003-0469-4321
AD  - Neuroimaging Research Branch, National Institute on Drug Abuse, Intramural 
      Research Program, NIH, Baltimore, MD, 21224, USA. luha@mail.nih.gov.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20210114
PL  - England
TA  - Commun Biol
JT  - Communications biology
JID - 101719179
RN  - 57-50-1 (Sucrose)
SB  - IM
MH  - Animals
MH  - Behavior, Animal/physiology
MH  - Brain/*diagnostic imaging/*physiology
MH  - Cues
MH  - Magnetic Resonance Imaging
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Optogenetics/*methods
MH  - Prefrontal Cortex/physiology
MH  - Sucrose
MH  - Mice
PMC - PMC7809041
COIS- H.L., Y.Y. and E.A.S. are the inventors of a patent (US9717946B2) issued to the 
      National Institutes of Health in 2017. The behavioral training method and imaging 
      platform were disclosed in the patent. The remaining authors declare no competing 
      interests.
EDAT- 2021/01/16 06:00
MHDA- 2021/07/10 06:00
PMCR- 2021/01/14
CRDT- 2021/01/15 05:57
PHST- 2020/03/12 00:00 [received]
PHST- 2020/12/01 00:00 [accepted]
PHST- 2021/01/15 05:57 [entrez]
PHST- 2021/01/16 06:00 [pubmed]
PHST- 2021/07/10 06:00 [medline]
PHST- 2021/01/14 00:00 [pmc-release]
AID - 10.1038/s42003-020-01612-x [pii]
AID - 1612 [pii]
AID - 10.1038/s42003-020-01612-x [doi]
PST - epublish
SO  - Commun Biol. 2021 Jan 14;4(1):66. doi: 10.1038/s42003-020-01612-x.