PMID- 33447967
OWN - NLM
STAT- MEDLINE
DCOM- 20210924
LR  - 20210924
IS  - 1543-706X (Electronic)
IS  - 1071-2690 (Print)
IS  - 1071-2690 (Linking)
VI  - 57
IP  - 1
DP  - 2021 Jan
TI  - Dexamethasone enhances CD163 expression in porcine IPKM immortalized macrophages.
PG  - 10-16
LID - 10.1007/s11626-020-00535-5 [doi]
AB  - In our previous study, we established a unique porcine macrophage cell line, 
      immortalized porcine kidney-derived macrophages (IPKM). The purpose of the 
      present study was to further elucidate the characteristics of IPKM. CD163 is a 
      scavenger receptor for the hemoglobin-haptoglobin complex and is used as a 
      phenotypic marker of anti-inflammatory M2 macrophages. The expression of CD163 is 
      enhanced by dexamethasone (DEX), a potent steroidal anti-inflammatory drug, in 
      human and rodent macrophages in vitro. Therefore, we investigated the effects of 
      DEX on CD163 expression in porcine IPKM. Treatment with DEX markedly enhanced 
      CD163 expression in the IPKM. In addition, we found that SB203580, a selective 
      inhibitor of p38 mitogen-activated protein kinase (MAPK), blocked the effects of 
      DEX, suggesting that the p38 MAPK signaling pathway is involved in the regulation 
      of the DEX-induced enhancement of CD163 expression. Since CD163 is considered to 
      be a putative receptor for the porcine reproductive and respiratory syndrome 
      virus (PRRSV), the effects of DEX on the infection of IPKM by PRRSV were 
      evaluated. Although the IPKM were susceptible to infection by the Fostera PRRSV 
      vaccine strain, DEX treatment did not affect the propagation of the virus in the 
      IPKM. This suggests that the DEX-induced enhancement of CD163 expression alone is 
      not sufficient to facilitate the infection of IPKM by PRRSV.
FAU - Takenouchi, Takato
AU  - Takenouchi T
AD  - Division of Animal Sciences, Institute of Agrobiological Sciences, National 
      Agriculture and Food Research Organization, 1-2 Ohwashi, Tsukuba, Ibaraki, 
      305-8634, Japan. ttakenou@affrc.go.jp.
FAU - Morozumi, Takeya
AU  - Morozumi T
AD  - Research & Development Center, NH Foods Ltd., 3-3 Midorigahara, Tsukuba, Ibaraki, 
      300-2646, Japan.
FAU - Wada, Emi
AU  - Wada E
AD  - Research & Development Center, NH Foods Ltd., 3-3 Midorigahara, Tsukuba, Ibaraki, 
      300-2646, Japan.
FAU - Suzuki, Shunichi
AU  - Suzuki S
AD  - Division of Animal Sciences, Institute of Agrobiological Sciences, National 
      Agriculture and Food Research Organization, 1-2 Ohwashi, Tsukuba, Ibaraki, 
      305-8634, Japan.
FAU - Nishiyama, Yasutaka
AU  - Nishiyama Y
AD  - Research & Development Center, NH Foods Ltd., 3-3 Midorigahara, Tsukuba, Ibaraki, 
      300-2646, Japan.
FAU - Sukegawa, Shin
AU  - Sukegawa S
AD  - Research & Development Center, NH Foods Ltd., 3-3 Midorigahara, Tsukuba, Ibaraki, 
      300-2646, Japan.
FAU - Uenishi, Hirohide
AU  - Uenishi H
AD  - Division of Animal Sciences, Institute of Agrobiological Sciences, National 
      Agriculture and Food Research Organization, 1-2 Ohwashi, Tsukuba, Ibaraki, 
      305-8634, Japan.
LA  - eng
PT  - Journal Article
DEP - 20210114
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (Butadienes)
RN  - 0 (CD163 antigen)
RN  - 0 (Imidazoles)
RN  - 0 (Nitriles)
RN  - 0 (Pyridines)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 1)
RN  - 0 (U 0126)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - OU13V1EYWQ (SB 203580)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*metabolism
MH  - Antigens, Differentiation, Myelomonocytic/*metabolism
MH  - Butadienes/pharmacology
MH  - Cell Line, Transformed
MH  - Cell Proliferation/drug effects
MH  - Dexamethasone/*pharmacology
MH  - Imidazoles/pharmacology
MH  - Kidney/*pathology
MH  - Macrophages/drug effects/*metabolism/virology
MH  - Nitriles/pharmacology
MH  - Porcine Reproductive and Respiratory Syndrome/pathology/virology
MH  - Porcine respiratory and reproductive syndrome virus/physiology
MH  - Pyridines/pharmacology
MH  - Receptors, Cell Surface/*metabolism
MH  - Sialic Acid Binding Ig-like Lectin 1/metabolism
MH  - Swine
PMC - PMC7862206
EDAT- 2021/01/16 06:00
MHDA- 2021/09/25 06:00
PMCR- 2021/01/14
CRDT- 2021/01/15 06:03
PHST- 2020/08/07 00:00 [received]
PHST- 2020/11/24 00:00 [accepted]
PHST- 2021/01/16 06:00 [pubmed]
PHST- 2021/09/25 06:00 [medline]
PHST- 2021/01/15 06:03 [entrez]
PHST- 2021/01/14 00:00 [pmc-release]
AID - 10.1007/s11626-020-00535-5 [pii]
AID - 535 [pii]
AID - 10.1007/s11626-020-00535-5 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2021 Jan;57(1):10-16. doi: 
      10.1007/s11626-020-00535-5. Epub 2021 Jan 14.