PMID- 33451062
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210218
IS  - 2309-608X (Electronic)
IS  - 2309-608X (Linking)
VI  - 7
IP  - 1
DP  - 2021 Jan 13
TI  - In Vitro and In Vivo Effect of Peptides Derived from 14-3-3 Paracoccidioides spp. 
      Protein.
LID - 10.3390/jof7010052 [doi]
LID - 52
AB  - BACKGROUND: Paracoccidioidomycosis (PCM) is a chronic disease that causes 
      sequelae and requires prolonged treatment; therefore, new therapeutic approaches 
      are necessary. In view of this, three peptides from Paracoccidioides brasiliensis 
      14-3-3 protein were selected based on its immunogenicity and therapeutic 
      potential. METHODS: The in vitro antifungal activity and cytotoxicity of the 
      14-3-3 peptides were evaluated. The influence of the peptides in immunological 
      and survival aspects was evaluated in vivo, using Galleria mellonella and the 
      expression of antimicrobial peptide genes in Caenorhabditis elegans. RESULTS: 
      None of the peptides were toxic to HaCaT (skin keratinocyte), MRC-5 (lung 
      fibroblast), and A549 (pneumocyte) cell lines, and only P1 exhibited antifungal 
      activity against Paracoccidioides spp. The peptides could induce an immune 
      response in G. mellonella. Moreover, the peptides caused a delay in the death of 
      Paracoccidioides spp. infected larvae. Regarding C. elegans, the three peptides 
      were able to increase the expression of the antimicrobial peptides. These 
      peptides had essential effects on different aspects of Paracoccidioides spp. 
      infection showing potential for a therapeutic vaccine. Future studies using 
      mammalian methods are necessary to validate our findings.
FAU - Scorzoni, Liliana
AU  - Scorzoni L
AUID- ORCID: 0000-0002-0178-6653
AD  - School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), 
      Araraquara, Sao Paulo 14800-903, Brazil.
FAU - Alves de Paula E Silva, Ana Carolina
AU  - Alves de Paula E Silva AC
AUID- ORCID: 0000-0001-5296-1514
AD  - School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), 
      Araraquara, Sao Paulo 14800-903, Brazil.
FAU - de Oliveira, Haroldo Cesar
AU  - de Oliveira HC
AUID- ORCID: 0000-0001-5760-4209
AD  - School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), 
      Araraquara, Sao Paulo 14800-903, Brazil.
FAU - Tavares Dos Santos, Claudia
AU  - Tavares Dos Santos C
AD  - School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), 
      Araraquara, Sao Paulo 14800-903, Brazil.
FAU - de Lacorte Singulani, Junya
AU  - de Lacorte Singulani J
AUID- ORCID: 0000-0002-7775-0657
AD  - School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), 
      Araraquara, Sao Paulo 14800-903, Brazil.
FAU - Akemi Assato, Patricia
AU  - Akemi Assato P
AD  - School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), 
      Araraquara, Sao Paulo 14800-903, Brazil.
FAU - Maria Marcos, Caroline
AU  - Maria Marcos C
AUID- ORCID: 0000-0001-9306-7404
AD  - School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), 
      Araraquara, Sao Paulo 14800-903, Brazil.
FAU - Teodoro Oliveira, Lariane
AU  - Teodoro Oliveira L
AD  - School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), 
      Araraquara, Sao Paulo 14800-903, Brazil.
FAU - Ferreira Fregonezi, Nathalia
AU  - Ferreira Fregonezi N
AD  - School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), 
      Araraquara, Sao Paulo 14800-903, Brazil.
FAU - Rossi, Diego Conrado Pereira
AU  - Rossi DCP
AUID- ORCID: 0000-0003-1385-1501
AD  - Department of Microbiology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo 05508-000, Brazil.
FAU - Buffoni Roque da Silva, Leandro
AU  - Buffoni Roque da Silva L
AD  - Department of Microbiology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo 05508-000, Brazil.
FAU - Pelleschi Taborda, Carlos
AU  - Pelleschi Taborda C
AUID- ORCID: 0000-0001-9928-9809
AD  - Department of Microbiology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo 05508-000, Brazil.
FAU - Fusco-Almeida, Ana Marisa
AU  - Fusco-Almeida AM
AD  - School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), 
      Araraquara, Sao Paulo 14800-903, Brazil.
FAU - Soares Mendes-Giannini, Maria Jose
AU  - Soares Mendes-Giannini MJ
AD  - School of Pharmaceutical Sciences, Sao Paulo State University (UNESP), 
      Araraquara, Sao Paulo 14800-903, Brazil.
LA  - eng
GR  - (LS) 2013/10917-9, (HdO) 2015/14023-8, (CMM) 2016/17048-4, (JLS) 2014/10446-9, 
      (MM-G) 2015/03700-9/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
GR  - -/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)/
GR  - -/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/
PT  - Journal Article
DEP - 20210113
PL  - Switzerland
TA  - J Fungi (Basel)
JT  - Journal of fungi (Basel, Switzerland)
JID - 101671827
PMC - PMC7828505
OTO - NOTNLM
OT  - 14-3-3 protein
OT  - Caenorhabditis elegans
OT  - Galleria mellonella
OT  - Paracoccidioides spp.
OT  - paracoccidioidomycosis
OT  - vaccine
COIS- The authors declare no conflict of interest.
EDAT- 2021/01/17 06:00
MHDA- 2021/01/17 06:01
PMCR- 2021/01/13
CRDT- 2021/01/16 01:02
PHST- 2020/12/14 00:00 [received]
PHST- 2020/12/28 00:00 [revised]
PHST- 2021/01/05 00:00 [accepted]
PHST- 2021/01/16 01:02 [entrez]
PHST- 2021/01/17 06:00 [pubmed]
PHST- 2021/01/17 06:01 [medline]
PHST- 2021/01/13 00:00 [pmc-release]
AID - jof7010052 [pii]
AID - jof-07-00052 [pii]
AID - 10.3390/jof7010052 [doi]
PST - epublish
SO  - J Fungi (Basel). 2021 Jan 13;7(1):52. doi: 10.3390/jof7010052.