PMID- 33455381
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 2373-9878 (Electronic)
IS  - 2373-9878 (Linking)
VI  - 6
IP  - 3
DP  - 2020 Mar 9
TI  - Probing the Influence of gamma-Sterilization on the Oxidation, Crystallization, 
      Sliding Wear Resistance, and Cytocompatibility of Chemically Modified 
      Graphene-Oxide-Reinforced HDPE/UHMWPE Nanocomposites and Wear Debris.
PG  - 1462-1475
LID - 10.1021/acsbiomaterials.9b01327 [doi]
AB  - Osteolysis and aseptic loosening due to wear at the articulating interfaces of 
      prosthetic joints are considered to be the key concerns for implant failure in 
      load-bearing orthopedic applications. In an effort to reduce the wear and 
      processing difficulties of ultrahigh-molecular-weight polyethylene (UHMWPE), our 
      research group recently developed high-density polyethylene (HDPE)/UHMWPE 
      nanocomposites with chemically modified graphene oxide (mGO). Considering the 
      importance of sterilization, this work explores the influence of gamma-ray dosage of 
      25 kGy on the clinically relevant performance-limiting properties of these newly 
      developed hybrid nanocomposites in vitro. Importantly, this work also probes into 
      the cytotoxic effects of the wear debris of different compositions and sizes on 
      MC3T3 murine osteoblasts and human mesenchymal stem cells (hMSCs). In particular, 
      gamma-ray-sterilized 1 wt % mGO-reinforced HDPE/UHMWPE nanocomposites exhibit an 
      improvement in the oxidation index (16%), free energy of immersion (-12.1 mN/m), 
      surface polarity (5.0%), and hardness (42%). Consequently, such enhancements 
      result in better tribological properties, especially coefficient of friction 
      (+13%) and wear resistance, when compared with UHMWPE. A spectrum of analyses 
      using transmission electron microscopy (TEM) and in vitro cytocompatibility 
      assessment demonstrate that phagocytosable (0.5-4.5 mum) sterilized 1 mGO wear 
      particles, when present in culture media at 5 mg/mL concentration, induce neither 
      significant reduction in MC3T3 murine osteoblast and hMSC growth nor cell 
      morphology phenotype, during 24, 48, and 72 h of incubation. Taken together, this 
      study suggests that gamma-ray-sterilized HDPE/UHMWPE/mGO nanocomposites can be 
      utilized as promising articulating surfaces for total joint replacements.
FAU - Sharma, Vidushi
AU  - Sharma V
AD  - Laboratory for Biomaterials, Materials Research Center, Indian Institute of 
      Science, Bangalore 560012, India.
FAU - Bose, Suryasarathi
AU  - Bose S
AD  - Department of Materials Engineering, Indian Institute of Science, Bangalore 
      560012, India.
FAU - Kundu, Biswanath
AU  - Kundu B
AD  - Bioceramics & Coating Division, CSIR-Central Glass & Ceramic Research Institute, 
      196 Raja S. C. Mullick Road, Kolkata 700032, India.
FAU - Bodhak, Subhadip
AU  - Bodhak S
AD  - Bioceramics & Coating Division, CSIR-Central Glass & Ceramic Research Institute, 
      196 Raja S. C. Mullick Road, Kolkata 700032, India.
FAU - Mitun, Das
AU  - Mitun D
AD  - Bioceramics & Coating Division, CSIR-Central Glass & Ceramic Research Institute, 
      196 Raja S. C. Mullick Road, Kolkata 700032, India.
AD  - Biomaterials and Corrosion Laboratory, Department of Materials Science and 
      Engineering, Tel-Aviv University, Ramat Aviv, Tel Aviv 6997801, Israel.
FAU - Balla, Vamsi Krishna
AU  - Balla VK
AD  - Bioceramics & Coating Division, CSIR-Central Glass & Ceramic Research Institute, 
      196 Raja S. C. Mullick Road, Kolkata 700032, India.
AD  - Materials Innovation Guild, Department of Mechanical Engineering, University of 
      Louisville, Louisville, Kentucky 40208, United States.
FAU - Basu, Bikramjit
AU  - Basu B
AUID- ORCID: 0000-0002-9154-5553
AD  - Laboratory for Biomaterials, Materials Research Center, Indian Institute of 
      Science, Bangalore 560012, India.
AD  - Centre for Biosystems Science and Engineering, Indian Institute of Science, 
      Bangalore 560012, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200228
PL  - United States
TA  - ACS Biomater Sci Eng
JT  - ACS biomaterials science & engineering
JID - 101654670
RN  - 0 (Oxides)
RN  - 0 (Polyethylenes)
RN  - 0 (graphene oxide)
RN  - 0 (ultra-high molecular weight polyethylene)
RN  - 7782-42-5 (Graphite)
RN  - 9002-88-4 (Polyethylene)
SB  - IM
MH  - Animals
MH  - Crystallization
MH  - *Graphite
MH  - Humans
MH  - Mice
MH  - *Nanocomposites
MH  - Oxides
MH  - Polyethylene
MH  - Polyethylenes
MH  - Sterilization
OTO - NOTNLM
OT  - HDPE/UHMWPE nanocomposite
OT  - cytotoxicity, gamma-ray sterilization
OT  - graphene oxide
OT  - in vitro
OT  - wear
EDAT- 2021/01/19 06:00
MHDA- 2021/05/15 06:00
CRDT- 2021/01/18 05:20
PHST- 2021/01/18 05:20 [entrez]
PHST- 2021/01/19 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
AID - 10.1021/acsbiomaterials.9b01327 [doi]
PST - ppublish
SO  - ACS Biomater Sci Eng. 2020 Mar 9;6(3):1462-1475. doi: 
      10.1021/acsbiomaterials.9b01327. Epub 2020 Feb 28.