PMID- 3345560 OWN - NLM STAT- MEDLINE DCOM- 19880414 LR - 20190705 IS - 0092-8674 (Print) IS - 0092-8674 (Linking) VI - 52 IP - 1 DP - 1988 Jan 15 TI - The Drosophila single-minded gene encodes a nuclear protein with sequence similarity to the per gene product. PG - 143-51 AB - Mutations in the single-minded (sim) gene of Drosophila result in the loss of the precursor cells giving rise to the midline cells of the embryonic central nervous system. We have examined the structure of the sim product by sequencing a sim cDNA clone, and have also determined the subcellular localization of the protein and its developmental expression by staining embryos with an antiserum against a sim fusion protein. The results indicate that sim is a nuclear protein specifically expressed along the midline of the neuroepithelium, the same subset of cells that are missing in the mutant. No similarity is observed between sim and any known nuclear protein, but, surprisingly, it is similar to the Drosophila period (per) locus gene product, which controls the periodicity of biological rhythms. FAU - Crews, S T AU - Crews ST AD - Department of Biological Sciences, Stanford University, California 94305. FAU - Thomas, J B AU - Thomas JB FAU - Goodman, C S AU - Goodman CS LA - eng SI - GENBANK/M19020 PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - Cell JT - Cell JID - 0413066 RN - 0 (Nuclear Proteins) RN - 9007-49-2 (DNA) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Cell Nucleus/analysis MH - Cloning, Molecular MH - DNA/genetics MH - Drosophila/embryology/*genetics MH - *Genes MH - Immunohistochemistry MH - Molecular Sequence Data MH - Mutation MH - Nervous System/embryology MH - Neurons/analysis/ultrastructure MH - Nuclear Proteins/analysis/*genetics MH - Periodicity MH - Repetitive Sequences, Nucleic Acid MH - Sequence Homology, Nucleic Acid EDAT- 1988/01/15 00:00 MHDA- 1988/01/15 00:01 CRDT- 1988/01/15 00:00 PHST- 1988/01/15 00:00 [pubmed] PHST- 1988/01/15 00:01 [medline] PHST- 1988/01/15 00:00 [entrez] AID - 0092-8674(88)90538-7 [pii] AID - 10.1016/0092-8674(88)90538-7 [doi] PST - ppublish SO - Cell. 1988 Jan 15;52(1):143-51. doi: 10.1016/0092-8674(88)90538-7.