PMID- 33456233
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220419
IS  - 0973-029X (Print)
IS  - 1998-393X (Electronic)
IS  - 0973-029X (Linking)
VI  - 24
IP  - 2
DP  - 2020 May-Aug
TI  - Comparison of cluster analysis of Porphyromonas gingivalis by arbitrarily 
      primed-polymerase chain reaction between healthy and chronic periodontitis 
      subjects.
PG  - 251-257
LID - 10.4103/jomfp.JOMFP_3_20 [doi]
AB  - INTRODUCTION: Periodontitis is a chronic destructive inflammatory disease of the 
      oral cavity. The main causative agent is presence of biofilm formed due to 
      different micro-organisms. Among different micro- organisms "red complex" 
      bacteria is known to be the main causative agent in progression of periodontitis. 
      Porphyromonas gingivalis out of the red the complex organism plays a major role 
      in progression of periodontitis. P. gingivalisis present in both in healthy and 
      diseased individuals. The difference in the strains will determine the virulence 
      factor of the organism and also progression of disease. Only few studies have 
      been done showing variation in strains present between healthy and diseased. 
      AIMS: To check the difference in heterogeneity of P. gingivalis in chronic 
      periodontitis and healthy individuals through Arbitrarily Primed-PCR (AP-PCR). 
      MATERIALS AND METHODS: A total of 400 subjects (200 each of chronic 
      periodontitisandhealthy individuals) were included. Sub-gingival plaque was 
      collected in the Reduced transport fluid (RTF) medium and processed at the 
      institutional central research laboratory. Presence of P. gingivalis was, 
      confirmed by culture andphenotypical analysis. Further confirmed cases were 
      processed for PCR after DNA extraction using 16S rRNA. Positive cases of P. 
      gingivalis were subjected for AP-PCR for clonal analysis using the specific 272 
      primer. RESULTS: In 152(76%) and 98(49%) were confirmed for P. gingivalis in 
      chronic periodontitis and healthy individual respectively by PCR. AP-PCR analysis 
      showed 6 clusters with similarity index in CP and 3 clusters with similarity 
      index in Healthy individuals. CONCLUSION: The present study showed difference in 
      clusters between chronic periodontitis and healthy individual'sthussuggestive 
      variantin genetic heterogeneity of P. gingivalis strain between healthy and 
      chronic periodontitis. AP- PCR appears to be a promising tool for clonal analysis 
      of P. gingivalis.
CI  - Copyright: (c) 2020 Journal of Oral and Maxillofacial Pathology.
FAU - Ingalagi, Preeti
AU  - Ingalagi P
AD  - Department of Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and 
      Research Centre, Belgaum, Karnataka, India.
FAU - Kotrashetti, Vijayalakshmi
AU  - Kotrashetti V
AD  - Department of Oral Pathology and Microbiology, Maratha Mandal's NGH Institute of 
      Dental Sciences and Research Centre, Belgaum, Karnataka, India.
FAU - Bhat, Kishore
AU  - Bhat K
AD  - Department of Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and 
      Research Centre, Belgaum, Karnataka, India.
FAU - Kugaji, Manohar
AU  - Kugaji M
AD  - Department of Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and 
      Research Centre, Belgaum, Karnataka, India.
LA  - eng
PT  - Journal Article
DEP - 20200909
PL  - India
TA  - J Oral Maxillofac Pathol
JT  - Journal of oral and maxillofacial pathology : JOMFP
JID - 101227995
PMC - PMC7802863
OTO - NOTNLM
OT  - Arbitrarily primed-polymerase chain reaction
OT  - Porphyromonas gingivalis
OT  - chronic periodontitis
OT  - cluster
OT  - healthy
OT  - subgingival plaque
COIS- There are no conflicts of interest.
EDAT- 2021/01/19 06:00
MHDA- 2021/01/19 06:01
PMCR- 2020/05/01
CRDT- 2021/01/18 05:28
PHST- 2020/01/11 00:00 [received]
PHST- 2020/05/06 00:00 [revised]
PHST- 2020/05/26 00:00 [accepted]
PHST- 2021/01/18 05:28 [entrez]
PHST- 2021/01/19 06:00 [pubmed]
PHST- 2021/01/19 06:01 [medline]
PHST- 2020/05/01 00:00 [pmc-release]
AID - JOMFP-24-251 [pii]
AID - 10.4103/jomfp.JOMFP_3_20 [doi]
PST - ppublish
SO  - J Oral Maxillofac Pathol. 2020 May-Aug;24(2):251-257. doi: 
      10.4103/jomfp.JOMFP_3_20. Epub 2020 Sep 9.