PMID- 33456328
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210119
IS  - 1426-3912 (Print)
IS  - 1644-4124 (Electronic)
IS  - 1426-3912 (Linking)
VI  - 45
IP  - 2
DP  - 2020
TI  - Non-classical human leukocyte antigen class I in Tunisian children with autism.
PG  - 176-183
LID - 10.5114/ceji.2020.97906 [doi]
AB  - Autism spectrum disorders (ASD) are one of the most common childhood morbidities 
      characterized by deficits in communication and social skills. Increasing evidence 
      has suggested associations between immune genes located in the human leukocyte 
      antigen (HLA) complex and etiology of autism. In this study, we investigated 
      whether the non-classical class I HLA-G, -E, and -F polymorphisms are associated 
      with genetic predisposition to autism in Tunisia. We aimed to find a correlation 
      between HLA-G genotypes and soluble HLA-G (sHLA-G) levels. We have analyzed the 
      HLA-G, -E, and -F genotypes of 15 autistic children and their parents. DNA typing 
      of HLA class I genes was performed using PCR-SSP and PCR-RFLP methods. Also, we 
      evaluated the serum levels of HLA-G (1 and 5) by a validated ELISA technique in 
      autistic probands and their parents. No association was found between any 
      polymorphism and autism in the study subjects. Additionally, we found no 
      correlation between sHLA-G1 and sHLA-G5 and autism. Also, no significant 
      difference in sHLA-G testing in parents and offspring was found. However, parents 
      carrying [GG] genotype presented a higher sHLA-G levels than those carrying 
      ([CC]+[GC]) genotypes (p = 0.037). From this preliminary study, we conclude that 
      the investigated polymorphisms of HLA-G, -E, and -F genes did not lead to autism 
      susceptibility in Tunisian children. However, the CGTIGA haplotype was found to 
      be associated with the disease.
CI  - Copyright (c) 2020 Termedia.
FAU - Kharrat, Najla
AU  - Kharrat N
AD  - Laboratory of Molecular and Cellular Screening Process, Centre of Biotechnology 
      of Sfax, Sfax University, Tunisia.
FAU - Abdelhedi, Rania
AU  - Abdelhedi R
AD  - Laboratory of Molecular and Cellular Screening Process, Centre of Biotechnology 
      of Sfax, Sfax University, Tunisia.
FAU - Gtif, Imen
AU  - Gtif I
AD  - Laboratory of Molecular and Cellular Screening Process, Centre of Biotechnology 
      of Sfax, Sfax University, Tunisia.
FAU - Ayadi, Imen
AU  - Ayadi I
AD  - Laboratory of Molecular and Cellular Screening Process, Centre of Biotechnology 
      of Sfax, Sfax University, Tunisia.
FAU - Rizzo, Roberta
AU  - Rizzo R
AD  - Laboratory of Molecular and Cellular Screening Process, Centre of Biotechnology 
      of Sfax, Sfax University, Tunisia.
FAU - Bortolotti, Daria
AU  - Bortolotti D
AD  - Laboratory of Molecular and Cellular Screening Process, Centre of Biotechnology 
      of Sfax, Sfax University, Tunisia.
FAU - Abdelmoula, Nouha Bouayed
AU  - Abdelmoula NB
AD  - Research Unit: UR17ES36 Genomics of Signalopathies in the Service of Medicine, 
      Sfax University, Medical University of Sfax, Tunisia.
FAU - Ghribi, Farhat
AU  - Ghribi F
AD  - Laboratory of Molecular and Cellular Screening Process, Centre of Biotechnology 
      of Sfax, Sfax University, Tunisia.
AD  - Department of Child and Adolescent Psychiatry, Hedi Chaker University Hospital, 
      Sfax, Tunisia.
FAU - Rebai, Ahmed
AU  - Rebai A
AD  - Laboratory of Molecular and Cellular Screening Process, Centre of Biotechnology 
      of Sfax, Sfax University, Tunisia.
FAU - Zidi, Ines
AU  - Zidi I
AD  - Laboratory Microorganismes and Active Biomolecules, Sciences Faculty of Tunis, 
      University of Tunis El Manar, Tunis, Tunisia.
LA  - eng
PT  - Journal Article
DEP - 20200727
PL  - Poland
TA  - Cent Eur J Immunol
JT  - Central-European journal of immunology
JID - 9702239
PMC - PMC7792448
OTO - NOTNLM
OT  - HLA-E
OT  - HLA-F
OT  - HLA-G
OT  - autism
OT  - polymorphism
COIS- The authors declare no conflict of interest.
EDAT- 2021/01/19 06:00
MHDA- 2021/01/19 06:01
PMCR- 2020/01/01
CRDT- 2021/01/18 05:28
PHST- 2018/03/05 00:00 [received]
PHST- 2018/10/16 00:00 [accepted]
PHST- 2021/01/18 05:28 [entrez]
PHST- 2021/01/19 06:00 [pubmed]
PHST- 2021/01/19 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 41512 [pii]
AID - 10.5114/ceji.2020.97906 [doi]
PST - ppublish
SO  - Cent Eur J Immunol. 2020;45(2):176-183. doi: 10.5114/ceji.2020.97906. Epub 2020 
      Jul 27.