PMID- 33456719
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210422
IS  - 1976-8354 (Print)
IS  - 2151-2485 (Electronic)
IS  - 1976-8354 (Linking)
VI  - 24
IP  - 6
DP  - 2020 Dec 24
TI  - Suppressive effects of valproic acid on caudal fin regeneration in adult 
      zebrafish.
PG  - 349-358
LID - 10.1080/19768354.2020.1860126 [doi]
AB  - Zebrafish can regenerate fins following injury through an epimorphic process that 
      includes the formation of new tissues and reconstruction of the original 
      morphology. In this study, the effects of valproic acid (VPA), a widely used 
      anti-epileptic drug, on fin regeneration were studied after the caudal fin 
      amputation of adult zebrafish. In the control group, zebrafish formed new tissues 
      and reconstructed the original rays 14 days after amputation (dpa). Meanwhile, 
      VPA treatments between 20 and 200 microM following amputation suppressed fin 
      regeneration in a dose-dependent manner and altered morphological 
      characteristics, such as bifurcation and segmentation, in the rays. Compared to 
      the control, VPA also delayed blastema formation and decreased cell proliferation 
      in the mesenchymal area of the regenerated fin. The mRNA expression of lef1, a 
      downstream signaling gene in the Wnt pathway, was transiently increased in the 
      regenerated fin of the control at 2 dpa; the same increase was not observed in 
      the VPA-treated zebrafish. Sodium butyrate (SB), an histone deacetylase activity 
      (HDAC) inhibitor, suppressed the fin regeneration without affecting the 
      morphological characteristics of the regenerated ray. Furthermore, the transient 
      increase of lef1 mRNA was not suppressed in the SB-treated zebrafish. These 
      results suggested that VPA's suppressive effects on fin regeneration are partly 
      mediated through decreased cell proliferation and lef1 mRNA expression.
CI  - (c) 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & 
      Francis Group.
FAU - Lee, Yunkyoung
AU  - Lee Y
AUID- ORCID: 0000-0002-3568-6204
AD  - Department of Biological Sciences, Inha University, Incheon, Korea.
FAU - Kim, Dohee
AU  - Kim D
AD  - Department of Biological Sciences, Inha University, Incheon, Korea.
FAU - Lee, Chang-Joong
AU  - Lee CJ
AD  - Department of Biological Sciences, Inha University, Incheon, Korea.
LA  - eng
PT  - Journal Article
DEP - 20201224
PL  - England
TA  - Anim Cells Syst (Seoul)
JT  - Animal cells and systems
JID - 101478641
PMC - PMC7782361
OTO - NOTNLM
OT  - BrdU
OT  - caudal fin
OT  - regeneration
OT  - valproic acid
OT  - zebrafish
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2021/01/19 06:00
MHDA- 2021/01/19 06:01
PMCR- 2020/12/24
CRDT- 2021/01/18 05:30
PHST- 2021/01/18 05:30 [entrez]
PHST- 2021/01/19 06:00 [pubmed]
PHST- 2021/01/19 06:01 [medline]
PHST- 2020/12/24 00:00 [pmc-release]
AID - 1860126 [pii]
AID - 10.1080/19768354.2020.1860126 [doi]
PST - epublish
SO  - Anim Cells Syst (Seoul). 2020 Dec 24;24(6):349-358. doi: 
      10.1080/19768354.2020.1860126.