PMID- 33461115 OWN - NLM STAT- MEDLINE DCOM- 20210407 LR - 20221111 IS - 1873-7072 (Electronic) IS - 0308-8146 (Print) IS - 0308-8146 (Linking) VI - 347 DP - 2021 Jun 15 TI - A visual bio-barcode immunoassay for sensitive detection of triazophos based on biochip silver staining signal amplification. PG - 129024 LID - S0308-8146(21)00025-X [pii] LID - 10.1016/j.foodchem.2021.129024 [doi] AB - Herein, a novel visual method for detecting triazophos based on competitive bio-barcode immunoassay was described. The competitive immunoassay was established by gold nanoparticles (AuNPs), magnetic microparticle (MMPs) and triazophos, combined with biochip hybridization system to detect the residual of triazophos in water and apple. Because AuNPs carried many bio-barcodes, which hybridized with labeled DNA on the biochip, catalyzed signal amplification using silver staining was detected by grayscale values as well as the naked eye. Notably, the grayscale values decreases with increasing the concentrations of triazophos, and the color change weakened gradually. The detection range was in between 0.05 and 10 ng/mL and the minimum detection limit was set at 0.04 ng/mL. Percent recovery calculated from spiked water and apple samples ranged between 55.4 and 107.8% with relative standard deviations (RSDs) of 12.4-24.9%. It has therefore been shown that this protocol provides a new insight for rapid detection of small molecule pesticides in various matrices. CI - Copyright (c) 2021 Elsevier Ltd. All rights reserved. FAU - Wang, Yuanshang AU - Wang Y AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. FAU - Du, Pengfei AU - Du P AD - Institute of Agro-Food Science and Technology Shandong Academy of Agricultural Sciences, Key Laboratory of Agro-Products Processing Technology of Shandong Province, Key Laboratory of Novel Food Resources Processing Ministry of Agriculture, Jinan 250100, PR China. Electronic address: dupengfei2011@163.com. FAU - Abd El-Aty, A M AU - Abd El-Aty AM AD - Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt; Department of Medical Pharmacology, Medical Faculty, Ataturk University, 25240 Erzurum, Turkey. Electronic address: abdelaty44@hotmail.com. FAU - Chen, Ge AU - Chen G AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. FAU - Jia, Huiyan AU - Jia H AD - Ningbo Academy of Agricultural Sciences, Ningbo 315040, PR China. FAU - Cui, Xueyan AU - Cui X AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. FAU - Oz, Emel AU - Oz E AD - Department of Food Engineering, Faculty of Agriculture, Ataturk University, 25240 Erzurum, Turkey. FAU - Zhang, Yudan AU - Zhang Y AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. FAU - Zhang, Xiuyuan AU - Zhang X AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. FAU - Qin, Guoxin AU - Qin G AD - Agro-products Quality Safety and Testing Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, PR China. FAU - Yan, Feiyan AU - Yan F AD - Agro-products Quality Safety and Testing Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, PR China. FAU - Wang, Jing AU - Wang J AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. FAU - Jin, Maojun AU - Jin M AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China; Department of Entomology & Nematology and the UC Davis Comprehensive Cancer Center, University of California, Davis, CA 95616, USA. Electronic address: jinmaojun@caas.cn. FAU - Hammock, Bruce D AU - Hammock BD AD - Department of Entomology & Nematology and the UC Davis Comprehensive Cancer Center, University of California, Davis, CA 95616, USA. LA - eng GR - P42 ES004699/ES/NIEHS NIH HHS/United States PT - Journal Article DEP - 20210109 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 RN - 0 (Organothiophosphates) RN - 0 (Triazoles) RN - 059QF0KO0R (Water) RN - 6099J8L0EE (triazophos) RN - 7440-57-5 (Gold) SB - IM MH - Gold MH - Immunoassay/*methods MH - Malus/*chemistry MH - Metal Nanoparticles/chemistry MH - Organothiophosphates/*analysis MH - Silver Staining MH - Triazoles/*analysis MH - Water/*chemistry PMC - PMC9648568 MID - NIHMS1836497 OTO - NOTNLM OT - Bio-barcode OT - Biochip silver staining OT - Gold nanoparticles OT - Immunoassay OT - Triazophos EDAT- 2021/01/19 06:00 MHDA- 2021/04/10 06:00 PMCR- 2022/11/10 CRDT- 2021/01/18 20:15 PHST- 2020/10/04 00:00 [received] PHST- 2021/01/01 00:00 [revised] PHST- 2021/01/02 00:00 [accepted] PHST- 2021/01/19 06:00 [pubmed] PHST- 2021/04/10 06:00 [medline] PHST- 2021/01/18 20:15 [entrez] PHST- 2022/11/10 00:00 [pmc-release] AID - S0308-8146(21)00025-X [pii] AID - 10.1016/j.foodchem.2021.129024 [doi] PST - ppublish SO - Food Chem. 2021 Jun 15;347:129024. doi: 10.1016/j.foodchem.2021.129024. Epub 2021 Jan 9.